GDF8_CHICK
ID GDF8_CHICK Reviewed; 375 AA.
AC O42220; Q53YW8; Q98SP0;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Growth/differentiation factor 8;
DE Short=GDF-8;
DE AltName: Full=Myostatin;
DE Flags: Precursor;
GN Name=MSTN; Synonyms=GDF8;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=White leghorn; TISSUE=Skeletal muscle;
RX PubMed=9356471; DOI=10.1073/pnas.94.23.12457;
RA McPherron A.C., Lee S.-J.;
RT "Double muscling in cattle due to mutations in the myostatin gene.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:12457-12461(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Skeletal muscle;
RA Ma X., Shi Z., Cao Y.;
RT "Cloning and sequencing analysis of myostatin from chicken and goose.";
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=15566475; DOI=10.1111/j.1365-2052.2004.01194.x;
RA Gu Z., Zhang Y., Shi P., Zhang Y.P., Zhu D., Li H.;
RT "Comparison of avian myostatin genes.";
RL Anim. Genet. 35:470-472(2004).
CC -!- FUNCTION: Acts specifically as a negative regulator of skeletal muscle
CC growth.
CC -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TGF-beta family. {ECO:0000305}.
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DR EMBL; AF019621; AAB86688.1; -; mRNA.
DR EMBL; AY448007; AAR18244.1; -; mRNA.
DR EMBL; AF346599; AAK18000.1; -; Genomic_DNA.
DR RefSeq; NP_001001461.1; NM_001001461.1.
DR AlphaFoldDB; O42220; -.
DR SMR; O42220; -.
DR STRING; 9031.ENSGALP00000003662; -.
DR PaxDb; O42220; -.
DR Ensembl; ENSGALT00000066640; ENSGALP00000045551; ENSGALG00000039458.
DR GeneID; 373964; -.
DR KEGG; gga:373964; -.
DR CTD; 2660; -.
DR VEuPathDB; HostDB:geneid_373964; -.
DR eggNOG; KOG3900; Eukaryota.
DR GeneTree; ENSGT00940000160657; -.
DR HOGENOM; CLU_020515_6_1_1; -.
DR InParanoid; O42220; -.
DR OMA; QICVYIY; -.
DR OrthoDB; 892873at2759; -.
DR PhylomeDB; O42220; -.
DR TreeFam; TF318514; -.
DR PRO; PR:O42220; -.
DR Proteomes; UP000000539; Chromosome 7.
DR Bgee; ENSGALG00000039458; Expressed in skeletal muscle tissue and 5 other tissues.
DR ExpressionAtlas; O42220; baseline.
DR GO; GO:0005615; C:extracellular space; IDA:AgBase.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0008201; F:heparin binding; IEA:Ensembl.
DR GO; GO:0042803; F:protein homodimerization activity; IEA:Ensembl.
DR GO; GO:0071549; P:cellular response to dexamethasone stimulus; IEA:Ensembl.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IEP:AgBase.
DR GO; GO:0046716; P:muscle cell cellular homeostasis; IEA:Ensembl.
DR GO; GO:0033002; P:muscle cell proliferation; IDA:AgBase.
DR GO; GO:0014839; P:myoblast migration involved in skeletal muscle regeneration; IEA:Ensembl.
DR GO; GO:0046627; P:negative regulation of insulin receptor signaling pathway; IEA:Ensembl.
DR GO; GO:0033673; P:negative regulation of kinase activity; IEA:Ensembl.
DR GO; GO:0045662; P:negative regulation of myoblast differentiation; IEA:Ensembl.
DR GO; GO:2000818; P:negative regulation of myoblast proliferation; ISS:AgBase.
DR GO; GO:0051898; P:negative regulation of protein kinase B signaling; IEA:Ensembl.
DR GO; GO:1902725; P:negative regulation of satellite cell differentiation; ISS:AgBase.
DR GO; GO:1902723; P:negative regulation of skeletal muscle satellite cell proliferation; ISS:AgBase.
DR GO; GO:0048632; P:negative regulation of skeletal muscle tissue growth; IEA:Ensembl.
DR GO; GO:0010592; P:positive regulation of lamellipodium assembly; IEA:Ensembl.
DR GO; GO:0010759; P:positive regulation of macrophage chemotaxis; IEA:Ensembl.
DR GO; GO:0010862; P:positive regulation of pathway-restricted SMAD protein phosphorylation; IBA:GO_Central.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:Ensembl.
DR GO; GO:0045595; P:regulation of cell differentiation; NAS:AgBase.
DR GO; GO:0040008; P:regulation of growth; NAS:AgBase.
DR GO; GO:0007519; P:skeletal muscle tissue development; IEP:AgBase.
DR GO; GO:0060395; P:SMAD protein signal transduction; IBA:GO_Central.
DR GO; GO:0007179; P:transforming growth factor beta receptor signaling pathway; IEA:Ensembl.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR015616; GDF_8.
DR InterPro; IPR001839; TGF-b_C.
DR InterPro; IPR001111; TGF-b_propeptide.
DR InterPro; IPR015615; TGF-beta-rel.
DR InterPro; IPR017948; TGFb_CS.
DR PANTHER; PTHR11848; PTHR11848; 1.
DR PANTHER; PTHR11848:SF150; PTHR11848:SF150; 1.
DR Pfam; PF00019; TGF_beta; 1.
DR Pfam; PF00688; TGFb_propeptide; 1.
DR SMART; SM00204; TGFB; 1.
DR SUPFAM; SSF57501; SSF57501; 1.
DR PROSITE; PS00250; TGF_BETA_1; 1.
DR PROSITE; PS51362; TGF_BETA_2; 1.
PE 2: Evidence at transcript level;
KW Cleavage on pair of basic residues; Cytokine; Disulfide bond; Glycoprotein;
KW Growth factor; Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT PROPEP 24..266
FT /evidence="ECO:0000255"
FT /id="PRO_0000033972"
FT CHAIN 267..375
FT /note="Growth/differentiation factor 8"
FT /id="PRO_0000033973"
FT CARBOHYD 71
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 272..282
FT /evidence="ECO:0000250|UniProtKB:O08689"
FT DISULFID 281..340
FT /evidence="ECO:0000250"
FT DISULFID 309..372
FT /evidence="ECO:0000250"
FT DISULFID 313..374
FT /evidence="ECO:0000250"
FT DISULFID 339
FT /note="Interchain"
FT /evidence="ECO:0000250"
FT CONFLICT 335
FT /note="S -> P (in Ref. 3; AAK18000)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 375 AA; 42707 MW; DA732DB9426E4D4F CRC64;
MQKLAVYVYI YLFMQIAVDP VALDGSSQPT ENAEKDGLCN ACTWRQNTKS SRIEAIKIQI
LSKLRLEQAP NISRDVIKQL LPKAPPLQEL IDQYDVQRDD SSDGSLEDDD YHATTETIIT
MPTESDFLVQ MEGKPKCCFF KFSSKIQYNK VVKAQLWIYL RQVQKPTTVF VQILRLIKPM
KDGTRYTGIR SLKLDMNPGT GIWQSIDVKT VLQNWLKQPE SNLGIEIKAF DETGRDLAVT
FPGPGEDGLN PFLEVRVTDT PKRSRRDFGL DCDEHSTESR CCRYPLTVDF EAFGWDWIIA
PKRYKANYCS GECEFVFLQK YPHTHLVHQA NPRGSAGPCC TPTKMSPINM LYFNGKEQII
YGKIPAMVVD RCGCS
