ID   GDIR1_DICDI             Reviewed;         197 AA.
AC   Q95UQ1; Q54F58;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Putative rho GDP-dissociation inhibitor 1;
GN   Name=rdiA; Synonyms=RhoGDI1; ORFNames=DDB_G0291077;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], INTERACTION WITH RAC1A; RAC1B; RAC1C;
RP   RACB; RACC AND RACE, AND FUNCTION.
RX   PubMed=12198156; DOI=10.1093/emboj/cdf449;
RA   Rivero F., Illenberger D., Somesh B.P., Dislich H., Adam N., Meyer A.-K.;
RT   "Defects in cytokinesis, actin reorganization and the contractile vacuole
RT   in cells deficient in RhoGDI.";
RL   EMBO J. 21:4539-4549(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   FUNCTION, AND INTERACTION WITH RAC1A; RAC1B; RAC1C; RACB; RACC AND RACE.
RX   PubMed=12163018; DOI=10.1016/s0006-291x(02)00861-6;
RA   Imai K., Kijima T., Noda Y., Sutoh K., Yoda K., Adachi H.;
RT   "A Rho GDP-dissociation inhibitor is involved in cytokinesis of
RT   Dictyostelium.";
RL   Biochem. Biophys. Res. Commun. 296:305-312(2002).
RN   [4]
RP   INDUCTION, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=19010323; DOI=10.1016/j.yexcr.2008.10.023;
RA   Sultana H., Neelakanta G., Eichinger L., Rivero F., Noegel A.A.;
RT   "Microarray phenotyping places cyclase associated protein CAP at the
RT   crossroad of signaling pathways reorganizing the actin cytoskeleton in
RT   Dictyostelium.";
RL   Exp. Cell Res. 315:127-140(2009).
CC   -!- FUNCTION: Regulates the GDP/GTP exchange reaction of the Rho proteins
CC       by inhibiting the dissociation of GDP from them, and the subsequent
CC       binding of GTP to them (By similarity). Regulates the Rac-dependent
CC       signaling pathways controlling cytokinesis, actin reorganization and
CC       the contractile vacuole. Required for efficient accumulation of cap at
CC       the cell cortex. {ECO:0000250, ECO:0000269|PubMed:12163018,
CC       ECO:0000269|PubMed:12198156, ECO:0000269|PubMed:19010323}.
CC   -!- SUBUNIT: Interacts with rac1A, rac1B, rac1C, racB, raCC and RacE.
CC       {ECO:0000269|PubMed:12163018, ECO:0000269|PubMed:12198156}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19010323}.
CC   -!- INDUCTION: Down-regulated in the absence of cap.
CC       {ECO:0000269|PubMed:19010323}.
CC   -!- SIMILARITY: Belongs to the Rho GDI family. {ECO:0000305}.
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DR   EMBL; AY044085; AAK95683.1; -; Genomic_DNA.
DR   EMBL; AAFI02000175; EAL61886.1; -; Genomic_DNA.
DR   RefSeq; XP_635396.1; XM_630304.1.
DR   AlphaFoldDB; Q95UQ1; -.
DR   SMR; Q95UQ1; -.
DR   STRING; 44689.DDB0216235; -.
DR   PaxDb; Q95UQ1; -.
DR   EnsemblProtists; EAL61886; EAL61886; DDB_G0291077.
DR   GeneID; 8627980; -.
DR   KEGG; ddi:DDB_G0291077; -.
DR   dictyBase; DDB_G0291077; rdiA.
DR   eggNOG; KOG3205; Eukaryota.
DR   HOGENOM; CLU_076228_1_2_1; -.
DR   InParanoid; Q95UQ1; -.
DR   OMA; HLKFEWS; -.
DR   PhylomeDB; Q95UQ1; -.
DR   Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR   Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR   Reactome; R-DDI-9013404; RAC2 GTPase cycle.
DR   Reactome; R-DDI-9013407; RHOH GTPase cycle.
DR   Reactome; R-DDI-9013423; RAC3 GTPase cycle.
DR   PRO; PR:Q95UQ1; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR   GO; GO:0005094; F:Rho GDP-dissociation inhibitor activity; IBA:GO_Central.
DR   GO; GO:0031267; F:small GTPase binding; TAS:dictyBase.
DR   GO; GO:0030036; P:actin cytoskeleton organization; IMP:dictyBase.
DR   GO; GO:0000902; P:cell morphogenesis; TAS:dictyBase.
DR   GO; GO:0000281; P:mitotic cytokinesis; IMP:dictyBase.
DR   GO; GO:0006907; P:pinocytosis; IMP:dictyBase.
DR   GO; GO:0035023; P:regulation of Rho protein signal transduction; IMP:dictyBase.
DR   GO; GO:0009617; P:response to bacterium; HEP:dictyBase.
DR   GO; GO:0007266; P:Rho protein signal transduction; IBA:GO_Central.
DR   Gene3D; 2.70.50.30; -; 1.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR000406; Rho_GDI.
DR   InterPro; IPR024792; RhoGDI_dom_sf.
DR   PANTHER; PTHR10980; PTHR10980; 1.
DR   Pfam; PF02115; Rho_GDI; 1.
DR   PRINTS; PR00492; RHOGDI.
DR   SUPFAM; SSF81296; SSF81296; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Cytoplasm; GTPase activation;
KW   Reference proteome.
FT   CHAIN           1..197
FT                   /note="Putative rho GDP-dissociation inhibitor 1"
FT                   /id="PRO_0000365602"
SQ   SEQUENCE   197 AA;  22340 MW;  88CFA6DB948241CE CRC64;
     MSDNHEEESN VPAYVPGKHV SVDQLKQQDA EDEALKRYKE SLLGTGVHAP KDDPRKLVIK
     EMKIQIEGRP DTIYPLDTKE LIKEMKTKPF VLKESCHYKI TLTFNIQHDI VSGLKQTNTV
     YRKGIKVSTE KHMLGSFAPQ ALAHSVTNPR HGWEEAPSGM LARGSYTAKV VFTDDDNEEH
     LSVEYAFSIK SDWKSDD