GDL16_ARATH
ID GDL16_ARATH Reviewed; 394 AA.
AC Q9C857; Q56XR2; Q68EB0; Q9C6U9;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=GDSL esterase/lipase At1g31550;
DE EC=3.1.1.-;
DE AltName: Full=Extracellular lipase At1g31550;
DE Flags: Precursor;
GN OrderedLocusNames=At1g31550; ORFNames=F27M3.22, T8E3.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP REVIEW.
RX PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT "GDSL family of serine esterases/lipases.";
RL Prog. Lipid Res. 43:534-552(2004).
RN [6]
RP GENE FAMILY.
RX PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA Ling H.;
RT "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL Pak. J. Biol. Sci. 11:763-767(2008).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9C857-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9C857-2; Sequence=VSP_036691;
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG60153.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC027135; AAG51269.1; -; Genomic_DNA.
DR EMBL; AC074360; AAG60153.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31371.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31372.1; -; Genomic_DNA.
DR EMBL; BT015320; AAT99799.1; -; mRNA.
DR EMBL; BT015719; AAU45217.1; -; mRNA.
DR EMBL; AK221611; BAD95190.1; -; mRNA.
DR RefSeq; NP_001031122.1; NM_001036045.3. [Q9C857-1]
DR RefSeq; NP_174440.2; NM_102894.4. [Q9C857-2]
DR AlphaFoldDB; Q9C857; -.
DR SMR; Q9C857; -.
DR STRING; 3702.AT1G31550.2; -.
DR PaxDb; Q9C857; -.
DR PRIDE; Q9C857; -.
DR ProteomicsDB; 221966; -. [Q9C857-1]
DR EnsemblPlants; AT1G31550.1; AT1G31550.1; AT1G31550. [Q9C857-2]
DR EnsemblPlants; AT1G31550.2; AT1G31550.2; AT1G31550. [Q9C857-1]
DR GeneID; 840045; -.
DR Gramene; AT1G31550.1; AT1G31550.1; AT1G31550. [Q9C857-2]
DR Gramene; AT1G31550.2; AT1G31550.2; AT1G31550. [Q9C857-1]
DR KEGG; ath:AT1G31550; -.
DR Araport; AT1G31550; -.
DR TAIR; locus:2028661; AT1G31550.
DR eggNOG; ENOG502QSMM; Eukaryota.
DR HOGENOM; CLU_015101_2_1_1; -.
DR InParanoid; Q9C857; -.
DR OMA; NSETHCR; -.
DR OrthoDB; 704138at2759; -.
DR PhylomeDB; Q9C857; -.
DR BioCyc; ARA:AT1G31550-MON; -.
DR PRO; PR:Q9C857; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C857; baseline and differential.
DR Genevisible; Q9C857; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR CDD; cd01837; SGNH_plant_lipase_like; 1.
DR Gene3D; 3.40.50.1110; -; 1.
DR InterPro; IPR001087; GDSL.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR InterPro; IPR035669; SGNH_plant_lipase-like.
DR Pfam; PF00657; Lipase_GDSL; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Hydrolase; Lipid degradation;
KW Lipid metabolism; Reference proteome; Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..394
FT /note="GDSL esterase/lipase At1g31550"
FT /id="PRO_0000367358"
FT ACT_SITE 43
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 345
FT /evidence="ECO:0000250"
FT ACT_SITE 348
FT /evidence="ECO:0000250"
FT CARBOHYD 138
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 290
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 322
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 300..303
FT /note="SKYG -> R (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_036691"
FT CONFLICT 111
FT /note="K -> R (in Ref. 4; BAD95190)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 394 AA; 43376 MW; D90EE1914D4740CD CRC64;
MASLDSHVLM KLGSLFLSTL FVSIVSSESQ CRNFESIISF GDSIADTGNL LGLSDHNNLP
MSAFPPYGET FFHHPTGRFS DGRLIIDFIA EFLGLPYVPP YFGSTNGNFE KGVNFAVASA
TALESSFLEE KGYHCPHNFS LGVQLKIFKQ SLPNLCGLPS DCRDMIGNAL ILMGEIGAND
YNFPFFQLRP LDEVKELVPL VISTISSAIT ELIGMGGRTF LVPGGFPLGC SVAFLTLHQT
SNMEEYDPLT GCLKWLNKFG EYHSEQLQEE LNRLRKLNPH VNIIYADYYN ASLRLGREPS
KYGFINRHLS ACCGVGGPYN FNLSRSCGSV GVEACSDPSK YVAWDGLHMT EAAHKSMADG
LVKGPYAIPP FDWSCLSSMI KKKEVVGNTI FFDE
