GDL20_ARATH
ID GDL20_ARATH Reviewed; 372 AA.
AC Q9C5N8; Q3ECQ5; Q9SYF8;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=GDSL esterase/lipase At1g54020;
DE EC=3.1.1.-;
DE AltName: Full=Extracellular lipase At1g54020;
DE Flags: Precursor;
GN OrderedLocusNames=At1g54020; ORFNames=F15I1.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP REVIEW.
RX PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT "GDSL family of serine esterases/lipases.";
RL Prog. Lipid Res. 43:534-552(2004).
RN [5]
RP GENE FAMILY.
RX PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA Ling H.;
RT "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL Pak. J. Biol. Sci. 11:763-767(2008).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9C5N8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9C5N8-2; Sequence=VSP_036692;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to a competing acceptor splice
CC site. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD25774.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006577; AAD25774.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33035.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33036.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33037.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58998.1; -; Genomic_DNA.
DR EMBL; AF348585; AAK15556.1; -; mRNA.
DR EMBL; AY054258; AAL06917.1; -; mRNA.
DR EMBL; AY132004; AAM91037.1; -; mRNA.
DR PIR; G96580; G96580.
DR RefSeq; NP_001077719.1; NM_001084250.1. [Q9C5N8-2]
DR RefSeq; NP_001319222.1; NM_001333625.1. [Q9C5N8-2]
DR RefSeq; NP_175804.1; NM_104279.4. [Q9C5N8-1]
DR RefSeq; NP_849805.1; NM_179474.1. [Q9C5N8-2]
DR AlphaFoldDB; Q9C5N8; -.
DR SMR; Q9C5N8; -.
DR BioGRID; 27065; 1.
DR STRING; 3702.AT1G54020.2; -.
DR PaxDb; Q9C5N8; -.
DR PRIDE; Q9C5N8; -.
DR ProteomicsDB; 221968; -. [Q9C5N8-1]
DR EnsemblPlants; AT1G54020.1; AT1G54020.1; AT1G54020. [Q9C5N8-2]
DR EnsemblPlants; AT1G54020.2; AT1G54020.2; AT1G54020. [Q9C5N8-1]
DR EnsemblPlants; AT1G54020.3; AT1G54020.3; AT1G54020. [Q9C5N8-2]
DR EnsemblPlants; AT1G54020.5; AT1G54020.5; AT1G54020. [Q9C5N8-2]
DR GeneID; 841840; -.
DR Gramene; AT1G54020.1; AT1G54020.1; AT1G54020. [Q9C5N8-2]
DR Gramene; AT1G54020.2; AT1G54020.2; AT1G54020. [Q9C5N8-1]
DR Gramene; AT1G54020.3; AT1G54020.3; AT1G54020. [Q9C5N8-2]
DR Gramene; AT1G54020.5; AT1G54020.5; AT1G54020. [Q9C5N8-2]
DR KEGG; ath:AT1G54020; -.
DR Araport; AT1G54020; -.
DR TAIR; locus:2014365; AT1G54020.
DR eggNOG; ENOG502S4IX; Eukaryota.
DR InParanoid; Q9C5N8; -.
DR OMA; FWEDPME; -.
DR OrthoDB; 704138at2759; -.
DR PhylomeDB; Q9C5N8; -.
DR PRO; PR:Q9C5N8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C5N8; baseline and differential.
DR Genevisible; Q9C5N8; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005773; C:vacuole; HDA:TAIR.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR CDD; cd01837; SGNH_plant_lipase_like; 1.
DR Gene3D; 3.40.50.1110; -; 1.
DR InterPro; IPR001087; GDSL.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR InterPro; IPR035669; SGNH_plant_lipase-like.
DR Pfam; PF00657; Lipase_GDSL; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Hydrolase; Lipid degradation;
KW Lipid metabolism; Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..372
FT /note="GDSL esterase/lipase At1g54020"
FT /id="PRO_0000367362"
FT ACT_SITE 40
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 314
FT /evidence="ECO:0000250"
FT ACT_SITE 317
FT /evidence="ECO:0000250"
FT CARBOHYD 161
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 280
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..86
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_036692"
SQ SEQUENCE 372 AA; 41801 MW; 8B822683092FF727 CRC64;
MECSSVSVLG ILLVFPLLHN LVTISGQNLP AVGLFTFGDS NFDAGNKKFL TSAPLPQNFW
PYGKSRDDPK GKFSDGKIVP DFIAKFMGIP HDLPPALKPG TDVSRGASFA VGSASILGSP
KDSLALNQQV RKFNQMISNW KVDYIQKSVF MISIGMEDYY NFTKNNPNAE VSAQQAFVTS
VTNRFKSDIN LLYSSGASKF VVHLLAPLGC LPIARQEFKT GNNCYEKLND LAKQHNAKIG
PILNEMAETK PDFQFTVFDF YNVILRRTQR NMNYRFSVTN ISCCGVGTHY AYGCGLPNVH
SKLCEYQRSY LYFDARHNTE KAQEAFAHLI FGADPNVIQP MNVRELMVYP VNEPMREFWE
DPMDEKLSLV QY
