GDL22_ARATH
ID GDL22_ARATH Reviewed; 408 AA.
AC Q3ECP6; Q8GXJ3; Q8LFA1; Q9ZVL5;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 95.
DE RecName: Full=GDSL esterase/lipase At1g54790;
DE EC=3.1.1.-;
DE AltName: Full=Extracellular lipase At1g54790;
DE Flags: Precursor;
GN OrderedLocusNames=At1g54790; ORFNames=T22H22.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-406 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [7]
RP REVIEW.
RX PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT "GDSL family of serine esterases/lipases.";
RL Prog. Lipid Res. 43:534-552(2004).
RN [8]
RP GENE FAMILY.
RX PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA Ling H.;
RT "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL Pak. J. Biol. Sci. 11:763-767(2008).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3ECP6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3ECP6-2; Sequence=VSP_036693;
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM61525.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BX842537; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC005388; AAC64890.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33147.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33148.1; -; Genomic_DNA.
DR EMBL; AK118209; BAC42831.1; -; mRNA.
DR EMBL; BT005698; AAO64118.1; -; mRNA.
DR EMBL; AY084964; AAM61525.1; ALT_INIT; mRNA.
DR EMBL; BX842537; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; A96590; A96590.
DR RefSeq; NP_001185228.1; NM_001198299.1.
DR RefSeq; NP_564668.1; NM_104354.4. [Q3ECP6-2]
DR RefSeq; NP_974029.1; NM_202300.2. [Q3ECP6-1]
DR AlphaFoldDB; Q3ECP6; -.
DR STRING; 3702.AT1G54790.2; -.
DR PaxDb; Q3ECP6; -.
DR PRIDE; Q3ECP6; -.
DR ProteomicsDB; 247087; -. [Q3ECP6-1]
DR EnsemblPlants; AT1G54790.1; AT1G54790.1; AT1G54790. [Q3ECP6-2]
DR EnsemblPlants; AT1G54790.2; AT1G54790.2; AT1G54790. [Q3ECP6-1]
DR GeneID; 841920; -.
DR Gramene; AT1G54790.1; AT1G54790.1; AT1G54790. [Q3ECP6-2]
DR Gramene; AT1G54790.2; AT1G54790.2; AT1G54790. [Q3ECP6-1]
DR KEGG; ath:AT1G54790; -.
DR Araport; AT1G54790; -.
DR TAIR; locus:2199496; AT1G54790.
DR eggNOG; ENOG502QRTJ; Eukaryota.
DR InParanoid; Q3ECP6; -.
DR OrthoDB; 704138at2759; -.
DR PhylomeDB; Q3ECP6; -.
DR BioCyc; ARA:AT1G54790-MON; -.
DR PRO; PR:Q3ECP6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q3ECP6; baseline and differential.
DR Genevisible; Q3ECP6; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR CDD; cd01837; SGNH_plant_lipase_like; 1.
DR Gene3D; 3.40.50.1110; -; 1.
DR InterPro; IPR001087; GDSL.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR InterPro; IPR035669; SGNH_plant_lipase-like.
DR Pfam; PF00657; Lipase_GDSL; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Hydrolase; Lipid degradation;
KW Lipid metabolism; Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..408
FT /note="GDSL esterase/lipase At1g54790"
FT /id="PRO_0000367364"
FT ACT_SITE 38
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 370
FT /evidence="ECO:0000250"
FT ACT_SITE 373
FT /evidence="ECO:0000250"
FT CARBOHYD 273
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 289
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 361
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 295..320
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074,
FT ECO:0000303|PubMed:14593172, ECO:0000303|Ref.5"
FT /id="VSP_036693"
FT CONFLICT 2
FT /note="N -> K (in Ref. 5; AAM61525)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 408 AA; 45381 MW; F24204F497576CE2 CRC64;
MNITKMKLFY VILFFISSLQ ISNSIDFNYP SAFNFGDSNS DTGDLVAGLG IRLDLPNGQN
SFKTSSQRFC DGRLVIDFLM DEMDLPFLNP YLDSLGLPNF KKGCNFAAAG STILPANPTS
VSPFSFDLQI SQFIRFKSRA IELLSKTGRK YEKYLPPIDY YSKGLYMIDI GQNDIAGAFY
SKTLDQVLAS IPSILETFEA GLKRLYEEGG RNIWIHNTGP LGCLAQNIAK FGTDSTKLDE
FGCVSSHNQA AKLFNLQLHA MSNKFQAQYP DANVTYVDIF SIKSNLIANY SRFGKHFTKP
LIDLNHLENV GYNKILNVLG FEKPLMACCG VGGAPLNYDS RITCGQTKVL DGISVTAKAC
NDSSEYINWD GIHYTEAANE FVSSQILTGK YSDPPFSDQM PFFLTLKF
