ID   GDL33_ARATH             Reviewed;         322 AA.
AC   Q9SIF3; F4IU86;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 2.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=GDSL esterase/lipase At2g04020;
DE            EC=3.1.1.-;
DE   AltName: Full=Extracellular lipase At2g04020;
DE   Flags: Precursor;
GN   OrderedLocusNames=At2g04020; ORFNames=F3C11.13;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   REVIEW.
RX   PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA   Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT   "GDSL family of serine esterases/lipases.";
RL   Prog. Lipid Res. 43:534-552(2004).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA   Ling H.;
RT   "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL   Pak. J. Biol. Sci. 11:763-767(2008).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD32921.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=AEC05776.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC007167; AAD32921.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; AEC05776.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; ANM62225.1; -; Genomic_DNA.
DR   PIR; G84453; G84453.
DR   RefSeq; NP_001324400.1; NM_001335212.1.
DR   RefSeq; NP_178485.1; NM_126437.2.
DR   AlphaFoldDB; Q9SIF3; -.
DR   PaxDb; Q9SIF3; -.
DR   PRIDE; Q9SIF3; -.
DR   EnsemblPlants; AT2G04020.2; AT2G04020.2; AT2G04020.
DR   GeneID; 814928; -.
DR   Gramene; AT2G04020.2; AT2G04020.2; AT2G04020.
DR   KEGG; ath:AT2G04020; -.
DR   Araport; AT2G04020; -.
DR   TAIR; locus:2049641; AT2G04020.
DR   HOGENOM; CLU_015101_0_1_1; -.
DR   InParanoid; Q9SIF3; -.
DR   OrthoDB; 704138at2759; -.
DR   PhylomeDB; Q9SIF3; -.
DR   PRO; PR:Q9SIF3; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q9SIF3; baseline and differential.
DR   Genevisible; Q9SIF3; AT.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1110; -; 1.
DR   InterPro; IPR001087; GDSL.
DR   InterPro; IPR036514; SGNH_hydro_sf.
DR   Pfam; PF00657; Lipase_GDSL; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..322
FT                   /note="GDSL esterase/lipase At2g04020"
FT                   /id="PRO_0000367374"
FT   ACT_SITE        50
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        296
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        299
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        260
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   322 AA;  35962 MW;  7BAF9D43EB467769 CRC64;
     MGLPSSLESY LLLILLSFLN VSTIYSSKPS KEEAVLFGGN FPAFYVIGDS LVDPGNNNHL
     PTMIRANYPP YGSDFEGGKA TGRFSNGKTI ADYIAIYYKL PLVPAYLGLS DDRKDTISTG
     MNYASAGCGI RRLTGKIAGK CLSLSKQVDL FEETIEKHLK TNFKTPYELR EHLAHSLFMT
     VIGVNDYAFF YTRLTDANDF ADELLHKFLK KIEKLHKLGA RKFFINNIKP LGCYPNIVAK
     TFMLGLRGPS TNRYSSNLLN TTGPCCPLDY DGSLTSSCKR RSKTCKAPDS THIFFDPRHP
     TQLANFMYSI ACFDERTICH VV