ID   GDL35_ARATH             Reviewed;         344 AA.
AC   O65921;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=GDSL esterase/lipase At2g19010;
DE            EC=3.1.1.-;
DE   AltName: Full=Extracellular lipase At2g19010;
DE   Flags: Precursor;
GN   OrderedLocusNames=At2g19010; ORFNames=F19F24.4, T20K24.2;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA   Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT   "Simultaneous high-throughput recombinational cloning of open reading
RT   frames in closed and open configurations.";
RL   Plant Biotechnol. J. 4:317-324(2006).
RN   [4]
RP   REVIEW.
RX   PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA   Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT   "GDSL family of serine esterases/lipases.";
RL   Prog. Lipid Res. 43:534-552(2004).
RN   [5]
RP   GENE FAMILY.
RX   PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA   Ling H.;
RT   "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL   Pak. J. Biol. Sci. 11:763-767(2008).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; AC002392; AAD12019.1; -; Genomic_DNA.
DR   EMBL; AC003673; AAM14888.1; -; Genomic_DNA.
DR   EMBL; CP002685; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; DQ056535; AAY78687.1; -; mRNA.
DR   PIR; T01629; T01629.
DR   AlphaFoldDB; O65921; -.
DR   SMR; O65921; -.
DR   STRING; 3702.AT2G19010.1; -.
DR   PaxDb; O65921; -.
DR   ProteomicsDB; 247090; -.
DR   EnsemblPlants; AT2G19010.2; AT2G19010.2; AT2G19010.
DR   Gramene; AT2G19010.2; AT2G19010.2; AT2G19010.
DR   Araport; AT2G19010; -.
DR   TAIR; locus:2044425; AT2G19010.
DR   eggNOG; ENOG502SIKN; Eukaryota.
DR   HOGENOM; CLU_015101_0_0_1; -.
DR   InParanoid; O65921; -.
DR   PhylomeDB; O65921; -.
DR   PRO; PR:O65921; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O65921; baseline and differential.
DR   Genevisible; O65921; AT.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01837; SGNH_plant_lipase_like; 1.
DR   Gene3D; 3.40.50.1110; -; 1.
DR   InterPro; IPR001087; GDSL.
DR   InterPro; IPR036514; SGNH_hydro_sf.
DR   InterPro; IPR035669; SGNH_plant_lipase-like.
DR   Pfam; PF00657; Lipase_GDSL; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..344
FT                   /note="GDSL esterase/lipase At2g19010"
FT                   /id="PRO_0000367376"
FT   ACT_SITE        33
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        311
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        314
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        303
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   344 AA;  38162 MW;  0DB442C0BAC1BB78 CRC64;
     MSKACWLVAA IIFTAATVVY GQQAPCFFVF GDSMSDNGNN NNLKSEAKVN FSPYGNDFPK
     GPTGRFSNGR TIPDIIGELS GFKDFIPPFA EASPEQAHTG MNYASGGSGL REETSEHLGD
     RISIRKQLQN HKTSITKANV PAERLQQCLY MINIGSNDYI NNYFMSKPYN TKRRYTPKQY
     AYSLIIIYRS HLKNLHRLGA RKVAVFGLSQ IGCTPKIMKS HSDGKICSRE VNEAVKIFNK
     NLDDLVMDFN KKVRGAKFTY VDLFSGGDPQ AFIFLGFKVG GKSCCTVNPG EELCVPNQPV
     CANRTEYVFW DDLHSTEATN MVVAKGSFDG IISKPYSIAQ LAKE