GDL4_ARATH
ID GDL4_ARATH Reviewed; 402 AA.
AC P0DKJ6; Q8LD23; Q9LNT7;
DT 14-OCT-2015, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2015, sequence version 1.
DT 03-AUG-2022, entry version 40.
DE RecName: Full=GDSL esterase/lipase At1g20120 {ECO:0000303|PubMed:18819574};
DE EC=3.1.1.- {ECO:0000305};
DE AltName: Full=Extracellular lipase At1g20120;
DE Flags: Precursor;
GN OrderedLocusNames=At1g20120 {ECO:0000312|Araport:AT1G20120};
GN ORFNames=T20H2.10 {ECO:0000312|EMBL:AAF79901.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP REVIEW.
RX PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT "GDSL family of serine esterases/lipases.";
RL Prog. Lipid Res. 43:534-552(2004).
RN [5]
RP GENE FAMILY.
RX PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA Ling H.;
RT "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL Pak. J. Biol. Sci. 11:763-767(2008).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79901.1; Type=Erroneous gene model prediction; Note=The predicted gene At1g20110 has been split into 2 genes: At1g20110 and At1g20120.; Evidence={ECO:0000305};
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DR EMBL; AC022472; AAF79901.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29938.1; -; Genomic_DNA.
DR EMBL; AY086248; AAM64323.1; -; mRNA.
DR PIR; H86334; H86334.
DR RefSeq; NP_564104.1; NM_101866.3.
DR AlphaFoldDB; P0DKJ6; -.
DR SMR; P0DKJ6; -.
DR STRING; 3702.AT1G20120.1; -.
DR PaxDb; P0DKJ6; -.
DR PRIDE; P0DKJ6; -.
DR ProteomicsDB; 224759; -.
DR EnsemblPlants; AT1G20120.1; AT1G20120.1; AT1G20120.
DR GeneID; 838601; -.
DR Gramene; AT1G20120.1; AT1G20120.1; AT1G20120.
DR KEGG; ath:AT1G20120; -.
DR Araport; AT1G20120; -.
DR TAIR; locus:2198666; AT1G20120.
DR eggNOG; ENOG502QW19; Eukaryota.
DR OMA; NFTADIC; -.
DR OrthoDB; 704138at2759; -.
DR PhylomeDB; P0DKJ6; -.
DR PRO; PR:P0DKJ6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P0DKJ6; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016298; F:lipase activity; IEA:InterPro.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR CDD; cd01837; SGNH_plant_lipase_like; 1.
DR Gene3D; 3.40.50.1110; -; 1.
DR InterPro; IPR001087; GDSL.
DR InterPro; IPR008265; Lipase_GDSL_AS.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR InterPro; IPR035669; SGNH_plant_lipase-like.
DR Pfam; PF00657; Lipase_GDSL; 1.
DR PROSITE; PS01098; LIPASE_GDSL_SER; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..402
FT /note="GDSL esterase/lipase At1g20120"
FT /id="PRO_0000367346"
FT REGION 41..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..64
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 85
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 375
FT /evidence="ECO:0000250"
FT ACT_SITE 378
FT /evidence="ECO:0000250"
FT CARBOHYD 73
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 314
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 367
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 8
FT /note="G -> C (in Ref. 3; AAM64323)"
FT /evidence="ECO:0000305"
FT CONFLICT 300
FT /note="K -> R (in Ref. 3; AAM64323)"
FT /evidence="ECO:0000305"
FT CONFLICT 402
FT /note="N -> NSM (in Ref. 3; AAM64323)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 402 AA; 44222 MW; 88EA57C0CE797703 CRC64;
MLQDRVSGSL SSSKISRCVL FLSLFCFFLL TMHASANRLQ RVPNPGPSPA PEPKPCPSPG
PNPAPATTKR THNTTFPAIF AFGDSILDTG NNDYILTLIK ANFLPYGMNF PDKVPTGRFC
NGKIPSDFIA DYIGVKPVVP AYLRPGLTQE DLLTGVSFAS GGSGYDPLTP IVVSAIPMSK
QLTYFQEYIE KVKGFVGKEK AEHIISKGLA IVVAGSDDLA NTYYGEHLEE FLYDIDTYTS
FMASSAASFA MQLYESGAKK IGFIGVSPIG CIPIQRTTRG GLKRKCADEL NFAAQLFNSK
LSTSLNELAK TMKNTTLVYI DIYSSFNDMI QNPKKYGFDE IDRGCCGTGL LELGPLCNKY
TSLLCKNVSS FMFWDSYHPT ERAYKILSQK FVENDMGPFY DN
