GDL50_ARATH
ID GDL50_ARATH Reviewed; 354 AA.
AC Q9SF94; Q84WD5; Q9SR58;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=GDSL esterase/lipase At3g09930;
DE EC=3.1.1.-;
DE AltName: Full=Extracellular lipase At3g09930;
DE Flags: Precursor;
GN OrderedLocusNames=At3g09930; ORFNames=F8A24.1, T22K18.26;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP REVIEW.
RX PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT "GDSL family of serine esterases/lipases.";
RL Prog. Lipid Res. 43:534-552(2004).
RN [5]
RP GENE FAMILY.
RX PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA Ling H.;
RT "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL Pak. J. Biol. Sci. 11:763-767(2008).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF04430.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC010927; AAF04430.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC015985; AAF23243.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74837.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM64288.1; -; Genomic_DNA.
DR EMBL; BT003945; AAO41990.1; -; mRNA.
DR RefSeq; NP_001326328.1; NM_001337851.1.
DR RefSeq; NP_187604.1; NM_111828.3.
DR AlphaFoldDB; Q9SF94; -.
DR SMR; Q9SF94; -.
DR STRING; 3702.AT3G09930.1; -.
DR PaxDb; Q9SF94; -.
DR PRIDE; Q9SF94; -.
DR ProteomicsDB; 221978; -.
DR EnsemblPlants; AT3G09930.1; AT3G09930.1; AT3G09930.
DR EnsemblPlants; AT3G09930.2; AT3G09930.2; AT3G09930.
DR GeneID; 820154; -.
DR Gramene; AT3G09930.1; AT3G09930.1; AT3G09930.
DR Gramene; AT3G09930.2; AT3G09930.2; AT3G09930.
DR KEGG; ath:AT3G09930; -.
DR Araport; AT3G09930; -.
DR TAIR; locus:2085054; AT3G09930.
DR eggNOG; ENOG502QU3Y; Eukaryota.
DR HOGENOM; CLU_015101_0_1_1; -.
DR InParanoid; Q9SF94; -.
DR OMA; NAMRIHK; -.
DR OrthoDB; 704138at2759; -.
DR PhylomeDB; Q9SF94; -.
DR PRO; PR:Q9SF94; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SF94; baseline and differential.
DR Genevisible; Q9SF94; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR CDD; cd01837; SGNH_plant_lipase_like; 1.
DR Gene3D; 3.40.50.1110; -; 1.
DR InterPro; IPR001087; GDSL.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR InterPro; IPR035669; SGNH_plant_lipase-like.
DR Pfam; PF00657; Lipase_GDSL; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..354
FT /note="GDSL esterase/lipase At3g09930"
FT /id="PRO_0000367391"
FT ACT_SITE 46
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 329
FT /evidence="ECO:0000250"
FT ACT_SITE 332
FT /evidence="ECO:0000250"
FT CARBOHYD 133
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 233
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 237
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 256
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 300
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 27
FT /note="P -> H (in Ref. 3; AAO41990)"
FT /evidence="ECO:0000305"
FT CONFLICT 260
FT /note="P -> Q (in Ref. 3; AAO41990)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 354 AA; 39829 MW; AF146F9571389A37 CRC64;
MELPKLLISL FLFSFSSFFL GAESDYPQHS NLKRLRPNRL FVFGDSYADT GNIRKSLSDS
WKIPYGITFP QKPSGRFSDG RVATDFLARY LGIKSPIPYT WKDYAGKERL LYGMNYAYGG
TGVFKTKDNP LPNMTTQIDY FQRVLAAGNI YSPSDLPSSL ALVSVAGNDY ATFLALKRPL
TELPAFMKQV VDQIAVNAMR IHKLGVNKIV IPSMQPLGCL PSITVFNSFQ RCNATDNAST
NLHNYLLHKA IARLNNETKP STFVVLDHYN AFLTVFKNKG PEPGVSRFGN PLKPCCVGVN
SSYDCSNVDE KGEKKYIICE DPKAAFFWDI FHPSEEGWRS VYSVLHKHLK AIWI
