ID   GDL51_ARATH             Reviewed;         363 AA.
AC   Q9LJP2;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=GDSL esterase/lipase At3g14220;
DE            EC=3.1.1.-;
DE   AltName: Full=Extracellular lipase At3g14220;
DE   Flags: Precursor;
GN   OrderedLocusNames=At3g14220; ORFNames=MLE3.1;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT   features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT   clones.";
RL   DNA Res. 7:217-221(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   REVIEW.
RX   PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA   Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT   "GDSL family of serine esterases/lipases.";
RL   Prog. Lipid Res. 43:534-552(2004).
RN   [5]
RP   GENE FAMILY.
RX   PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA   Ling H.;
RT   "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL   Pak. J. Biol. Sci. 11:763-767(2008).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; AP000416; BAB01435.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE75488.1; -; Genomic_DNA.
DR   EMBL; BT004323; AAO42319.1; -; mRNA.
DR   EMBL; BT005625; AAO64045.1; -; mRNA.
DR   RefSeq; NP_188038.2; NM_112279.4.
DR   AlphaFoldDB; Q9LJP2; -.
DR   SMR; Q9LJP2; -.
DR   BioGRID; 5974; 1.
DR   IntAct; Q9LJP2; 1.
DR   STRING; 3702.AT3G14220.1; -.
DR   PaxDb; Q9LJP2; -.
DR   PRIDE; Q9LJP2; -.
DR   ProteomicsDB; 224760; -.
DR   EnsemblPlants; AT3G14220.1; AT3G14220.1; AT3G14220.
DR   GeneID; 820640; -.
DR   Gramene; AT3G14220.1; AT3G14220.1; AT3G14220.
DR   KEGG; ath:AT3G14220; -.
DR   Araport; AT3G14220; -.
DR   TAIR; locus:2090780; AT3G14220.
DR   eggNOG; ENOG502S4IX; Eukaryota.
DR   HOGENOM; CLU_015101_7_0_1; -.
DR   InParanoid; Q9LJP2; -.
DR   OMA; HPNANTV; -.
DR   OrthoDB; 704138at2759; -.
DR   PhylomeDB; Q9LJP2; -.
DR   PRO; PR:Q9LJP2; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LJP2; baseline and differential.
DR   Genevisible; Q9LJP2; AT.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR   GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01837; SGNH_plant_lipase_like; 1.
DR   Gene3D; 3.40.50.1110; -; 1.
DR   InterPro; IPR001087; GDSL.
DR   InterPro; IPR036514; SGNH_hydro_sf.
DR   InterPro; IPR035669; SGNH_plant_lipase-like.
DR   Pfam; PF00657; Lipase_GDSL; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Lipid degradation; Lipid metabolism; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..363
FT                   /note="GDSL esterase/lipase At3g14220"
FT                   /id="PRO_0000367392"
FT   ACT_SITE        39
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        318
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        321
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   363 AA;  40249 MW;  7CA4D1D1D0A65728 CRC64;
     MAKNRNLVFF LGVLASFTLS SFPVTVSGEP PILFTFGDSS YDVGNTKFFS SEFDPATTWP
     YGDSIDDPSG RWSDGHIVPD FVGRLIGHRE PIPPVLDPKA DLSRGASFAI AGAVVLGSQS
     TTASMNFGQQ ISKFLELHKQ WTDKERAEAI YMVNIGAEDY LNFAKAHPNA NTVEQLTQVA
     HVLQRIPREL TSLYRAGGAR KFAVQNLGPL GCLPIVRQEF KTGENCMEMV NFMVKTHNER
     LSRLLVAITV PLLYRGFRYS LFDFNGEILR RINEPSLHGY TDTTTSCCGT GSRNAYGCGY
     SNVHAKLCSY QKSFLFFDGR HNTEKTDEEV ANLFYSGDKH VVSPMNIKDL VGKAATDLLA
     QEI