ID   GDL54_ARATH             Reviewed;         371 AA.
AC   Q9LII9; F4IXS5;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=GDSL esterase/lipase At3g27950;
DE            EC=3.1.1.-;
DE   AltName: Full=Extracellular lipase At3g27950;
DE   Flags: Precursor;
GN   OrderedLocusNames=At3g27950; ORFNames=K24A2.4;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT   features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT   clones.";
RL   DNA Res. 7:217-221(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   REVIEW.
RX   PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA   Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT   "GDSL family of serine esterases/lipases.";
RL   Prog. Lipid Res. 43:534-552(2004).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA   Ling H.;
RT   "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL   Pak. J. Biol. Sci. 11:763-767(2008).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP001302; BAB01482.1; -; Genomic_DNA.
DR   EMBL; CP002686; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; Q9LII9; -.
DR   SMR; Q9LII9; -.
DR   STRING; 3702.AT3G27950.1; -.
DR   PaxDb; Q9LII9; -.
DR   PRIDE; Q9LII9; -.
DR   ProteomicsDB; 224762; -.
DR   Araport; AT3G27950; -.
DR   eggNOG; ENOG502QQSM; Eukaryota.
DR   HOGENOM; CLU_015101_2_0_1; -.
DR   InParanoid; Q9LII9; -.
DR   OrthoDB; 704138at2759; -.
DR   PhylomeDB; Q9LII9; -.
DR   BioCyc; ARA:AT3G27950-MON; -.
DR   PRO; PR:Q9LII9; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LII9; baseline and differential.
DR   Genevisible; Q9LII9; AT.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01837; SGNH_plant_lipase_like; 1.
DR   Gene3D; 3.40.50.1110; -; 1.
DR   InterPro; IPR001087; GDSL.
DR   InterPro; IPR036514; SGNH_hydro_sf.
DR   InterPro; IPR035669; SGNH_plant_lipase-like.
DR   Pfam; PF00657; Lipase_GDSL; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..371
FT                   /note="GDSL esterase/lipase At3g27950"
FT                   /id="PRO_0000367395"
FT   ACT_SITE        39
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        334
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        337
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        82
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        143
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        178
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        194
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        315
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   371 AA;  40778 MW;  0F67C0F4AEDFEADE CRC64;
     MAISKITLAI IVLLLGFTEK LSALSSSCNF PAVFNFGDSN SDTGAISAAI GEVPPPNGVA
     FFGRSAGRHS DGRLIIDFIT ENLTLPYLTP YLDSVGANYR HGANFATGGS CIRPTLACFS
     PFHLGTQVSQ FIHFKTRTLS LYNQTNGKFN RLSHTNYFSK ALYTLDIGQN DLAIGFQNMT
     EEQLKATIPL IIENFTIALK LLYKEGARFF SIHNTGPTGC LPYLLKAFPA IPRDPYGCLK
     PLNNVAIEFN KQLKNKITQL KKELPSSFFT YVDVYSAKYN LITKAKALGF IDPFDYCCVG
     AIGRGMGCGK TIFLNGTELY SSSCQNRKNF ISWDGIHYTE TANMLVANRI LDGSISDPPL
     PTQKACKLTK K