ID   GDL75_ARATH             Reviewed;         385 AA.
AC   Q9FNP2;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=GDSL esterase/lipase At5g08460;
DE            EC=3.1.1.-;
DE   AltName: Full=Extracellular lipase At5g08460;
DE   Flags: Precursor;
GN   OrderedLocusNames=At5g08460; ORFNames=F8L15.13, MAH20.2;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA   Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT   features of the regions of 1,044,062 bp covered by thirteen physically
RT   assigned P1 clones.";
RL   DNA Res. 4:291-300(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   REVIEW.
RX   PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA   Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT   "GDSL family of serine esterases/lipases.";
RL   Prog. Lipid Res. 43:534-552(2004).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA   Ling H.;
RT   "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL   Pak. J. Biol. Sci. 11:763-767(2008).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; AB006697; BAB09995.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED91306.1; -; Genomic_DNA.
DR   RefSeq; NP_196463.1; NM_120931.2.
DR   AlphaFoldDB; Q9FNP2; -.
DR   SMR; Q9FNP2; -.
DR   iPTMnet; Q9FNP2; -.
DR   PaxDb; Q9FNP2; -.
DR   PRIDE; Q9FNP2; -.
DR   ProteomicsDB; 247107; -.
DR   EnsemblPlants; AT5G08460.1; AT5G08460.1; AT5G08460.
DR   GeneID; 830745; -.
DR   Gramene; AT5G08460.1; AT5G08460.1; AT5G08460.
DR   KEGG; ath:AT5G08460; -.
DR   Araport; AT5G08460; -.
DR   TAIR; locus:2159547; AT5G08460.
DR   eggNOG; ENOG502QSW9; Eukaryota.
DR   HOGENOM; CLU_015101_0_0_1; -.
DR   InParanoid; Q9FNP2; -.
DR   OMA; CYPMNVK; -.
DR   OrthoDB; 704138at2759; -.
DR   PhylomeDB; Q9FNP2; -.
DR   BioCyc; ARA:AT5G08460-MON; -.
DR   PRO; PR:Q9FNP2; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FNP2; baseline and differential.
DR   Genevisible; Q9FNP2; AT.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01837; SGNH_plant_lipase_like; 1.
DR   Gene3D; 3.40.50.1110; -; 1.
DR   InterPro; IPR001087; GDSL.
DR   InterPro; IPR036514; SGNH_hydro_sf.
DR   InterPro; IPR035669; SGNH_plant_lipase-like.
DR   Pfam; PF00657; Lipase_GDSL; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000255"
FT   CHAIN           36..385
FT                   /note="GDSL esterase/lipase At5g08460"
FT                   /id="PRO_0000367415"
FT   ACT_SITE        56
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        350
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        353
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        218
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        285
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        368
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        378
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   385 AA;  42417 MW;  75873A7DACC830AC CRC64;
     MHDSEIFKFK DMMMMSCTVQ TLVLVPWFLV VFVLAGGEDS SETTAMFPAM FVFGDSLVDN
     GNNNHLNSLA RSNYLPYGID FAGNQPTGRF SNGKTIVDFI GELLGLPEIP AFMDTVDGGV
     DILHGVNYAS AAGGILEETG RHLGERFSMG RQVENFEKTL MEISRSMRKE SVKEYMAKSL
     VVVSLGNNDY INNYLKPRLF LSSSIYDPTS FADLLLSNFT THLLELYGKG FRKFVIAGVG
     PLGCIPDQLA AQAALPGECV EAVNEMAELF NNRLVSLVDR LNSDNKTASE AIFVYGNTYG
     AAVDILTNPF NYGFEVTDRG CCGVGRNRGE ITCLPLAVPC AFRDRHVFWD AFHPTQAFNL
     IIALRAFNGS KSDCYPINLS QLSRL