ID   GDL78_ARATH             Reviewed;         362 AA.
AC   Q9FFC6; F4KBC2;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 3.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=GDSL esterase/lipase At5g22810;
DE            EC=3.1.1.-;
DE   AltName: Full=Extracellular lipase At5g22810;
DE   Flags: Precursor;
GN   OrderedLocusNames=At5g22810; ORFNames=MRN17.4;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA   Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA   Miyajima N., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT   features of the 1.6 Mb regions covered by twenty physically assigned P1
RT   clones.";
RL   DNA Res. 4:215-230(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   REVIEW.
RX   PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA   Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT   "GDSL family of serine esterases/lipases.";
RL   Prog. Lipid Res. 43:534-552(2004).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA   Ling H.;
RT   "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL   Pak. J. Biol. Sci. 11:763-767(2008).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB10602.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB005243; BAB10602.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP002688; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; Q9FFC6; -.
DR   SMR; Q9FFC6; -.
DR   STRING; 3702.AT5G22810.1; -.
DR   PaxDb; Q9FFC6; -.
DR   PRIDE; Q9FFC6; -.
DR   ProteomicsDB; 247109; -.
DR   Araport; AT5G22810; -.
DR   eggNOG; KOG0017; Eukaryota.
DR   HOGENOM; CLU_015101_0_1_1; -.
DR   InParanoid; Q9FFC6; -.
DR   OrthoDB; 704138at2759; -.
DR   BioCyc; ARA:AT5G22810-MON; -.
DR   PRO; PR:Q9FFC6; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FFC6; baseline and differential.
DR   Genevisible; Q9FFC6; AT.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01837; SGNH_plant_lipase_like; 1.
DR   Gene3D; 3.40.50.1110; -; 1.
DR   InterPro; IPR001087; GDSL.
DR   InterPro; IPR036514; SGNH_hydro_sf.
DR   InterPro; IPR035669; SGNH_plant_lipase-like.
DR   Pfam; PF00657; Lipase_GDSL; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..362
FT                   /note="GDSL esterase/lipase At5g22810"
FT                   /id="PRO_0000367418"
FT   ACT_SITE        44
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        337
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        340
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        159
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        162
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        264
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        329
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   362 AA;  39540 MW;  C1ED0BDD430792BA CRC64;
     MGFSGIWLNL YVVFGSLMVF ERMVVMVVMK AQPLVPAIFI FGDSVVDVGN NNDIYTIVKA
     NFPPYGRDFT THTPTGRFCN GKLATDFTAE NLGFKSYPQA YLSKKAKGKN LLIGANFASA
     ASGYYDGTAK LYSAISLPQQ LEHYKDYISR IQEIATSNNN SNASAIISNG IYIVSAGSSD
     FIQNYYINPL LYRDQSPDEF SDLLILSYSS FIQNLYSLGA RRIGVTTLPP LGCLPAAITV
     VGPHEGGCSE KLNNDAISFN NKLNTTSQDL KRNLIGLNLV VFDIYQPLYD LATRPSEFGF
     AEARRACCGT GLLETSILCN PKSVGTCNNA TEYVFWDGFH PTEAANKILA DNLLVSGISL
     IS