ID   GDL89_ARATH             Reviewed;         338 AA.
AC   Q9FMK6;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=GDSL esterase/lipase At5g63170;
DE            EC=3.1.1.-;
DE   AltName: Full=Extracellular lipase At5g63170;
DE   Flags: Precursor;
GN   OrderedLocusNames=At5g63170; ORFNames=MDC12.14;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA   Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT   features of the regions of 1,191,918 bp covered by seventeen physically
RT   assigned P1 clones.";
RL   DNA Res. 4:401-414(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   REVIEW.
RX   PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA   Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT   "GDSL family of serine esterases/lipases.";
RL   Prog. Lipid Res. 43:534-552(2004).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA   Ling H.;
RT   "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL   Pak. J. Biol. Sci. 11:763-767(2008).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; AB008265; BAB10559.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97714.1; -; Genomic_DNA.
DR   RefSeq; NP_201122.1; NM_125712.1.
DR   AlphaFoldDB; Q9FMK6; -.
DR   SMR; Q9FMK6; -.
DR   PaxDb; Q9FMK6; -.
DR   PRIDE; Q9FMK6; -.
DR   ProteomicsDB; 247114; -.
DR   EnsemblPlants; AT5G63170.1; AT5G63170.1; AT5G63170.
DR   GeneID; 836438; -.
DR   Gramene; AT5G63170.1; AT5G63170.1; AT5G63170.
DR   KEGG; ath:AT5G63170; -.
DR   Araport; AT5G63170; -.
DR   TAIR; locus:2161977; AT5G63170.
DR   HOGENOM; CLU_015101_0_1_1; -.
DR   InParanoid; Q9FMK6; -.
DR   OMA; PAYRSPF; -.
DR   OrthoDB; 704138at2759; -.
DR   PhylomeDB; Q9FMK6; -.
DR   BioCyc; ARA:AT5G63170-MON; -.
DR   PRO; PR:Q9FMK6; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FMK6; baseline.
DR   GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR   GO; GO:0016298; F:lipase activity; IEA:InterPro.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01837; SGNH_plant_lipase_like; 1.
DR   Gene3D; 3.40.50.1110; -; 1.
DR   InterPro; IPR001087; GDSL.
DR   InterPro; IPR008265; Lipase_GDSL_AS.
DR   InterPro; IPR036514; SGNH_hydro_sf.
DR   InterPro; IPR035669; SGNH_plant_lipase-like.
DR   Pfam; PF00657; Lipase_GDSL; 1.
DR   PROSITE; PS01098; LIPASE_GDSL_SER; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Lipid degradation; Lipid metabolism; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..338
FT                   /note="GDSL esterase/lipase At5g63170"
FT                   /id="PRO_0000367429"
FT   ACT_SITE        35
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        313
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        316
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   338 AA;  36913 MW;  BE7BF71CF3830B5F CRC64;
     MNSLVIQTTI VLVSVISVSI VHAGNIPAVI AFGDSILDTG NNNYLMTLTK VNFYPYGRDF
     VTRRATGRFG NGRIPTDLIA EGLGIKNIVP AYRSPFLEPN DILTGVSFAS GGSGLDPMTA
     RIQGVIWVPD QLNDFKAYIA KLNSITGDEE KTRSIISNAV FVISAGNNDI AITYFTNPIR
     NTRYTIFSYT DLMVSWTQSF IKELYNLGAR KFAIMGTLPL GCLPGASNAL GGLCLEPANA
     VARLFNRKLA DEVNNLNSML PGSRSIYVDM YNPLLELVKN PLRSGFISPT RPCCCAPAAP
     IPCLDASRYV FWDIAHPSEK AYQTIIPPII QQIQQSFA