ID   GDNC_BACSU              Reviewed;         112 AA.
AC   P49856;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Probable guanidinium efflux system subunit GdnC {ECO:0000305};
GN   Name=gdnC {ECO:0000312|EMBL:CAB13166.1}; Synonyms=ykkC;
GN   OrderedLocusNames=BSU13090;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8682784; DOI=10.1128/jb.178.13.3803-3808.1996;
RA   Lacelle M., Kumano M., Kurita K., Yamane K., Zuber P., Nakano M.M.;
RT   "Oxygen-controlled regulation of the flavohemoglobin gene in Bacillus
RT   subtilis.";
RL   J. Bacteriol. 178:3803-3808(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Devine K.M.;
RT   "Sequence of the Bacillus subtilis genome between xlyA and ykoR.";
RL   Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   FUNCTION, AND SUBUNIT.
RX   PubMed=10735877; DOI=10.1128/jb.182.8.2311-2313.2000;
RA   Jack D.L., Storms M.L., Tchieu J.H., Paulsen I.T., Saier M.H. Jr.;
RT   "A broad-specificity multidrug efflux pump requiring a pair of homologous
RT   SMR-type proteins.";
RL   J. Bacteriol. 182:2311-2313(2000).
RN   [5]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=27989440; DOI=10.1016/j.molcel.2016.11.019;
RA   Nelson J.W., Atilho R.M., Sherlock M.E., Stockbridge R.B., Breaker R.R.;
RT   "Metabolism of free guanidine in bacteria is regulated by a widespread
RT   riboswitch class.";
RL   Mol. Cell 65:220-230(2017).
CC   -!- FUNCTION: Probably involved in guanidinium transport (PubMed:27989440).
CC       In vitro, confers resistance to a broad range of toxic compounds such
CC       as cationic dyes, neutral and anionic antimicrobials (PubMed:10735877).
CC       {ECO:0000269|PubMed:10735877, ECO:0000269|PubMed:27989440}.
CC   -!- SUBUNIT: The efflux pump is composed of GdnC and GdnD.
CC       {ECO:0000305|PubMed:10735877}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- INDUCTION: Transcriptionally regulated by guanidine, via a guanidine-
CC       sensing riboswitch. {ECO:0000269|PubMed:27989440}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of both gdnC and gdnD leads to a
CC       decrease in the concentration of guanidine required to inhibit
CC       bacterial growth. {ECO:0000269|PubMed:27989440}.
CC   -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC       superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
CC       YkkC/YkkD subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D78189; BAA11262.1; -; Genomic_DNA.
DR   EMBL; AJ002571; CAA05588.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB13166.1; -; Genomic_DNA.
DR   PIR; A69857; A69857.
DR   RefSeq; NP_389192.1; NC_000964.3.
DR   RefSeq; WP_003232583.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; P49856; -.
DR   SMR; P49856; -.
DR   STRING; 224308.BSU13090; -.
DR   TCDB; 2.A.7.1.5; the drug/metabolite transporter (dmt) superfamily.
DR   PaxDb; P49856; -.
DR   DNASU; 936835; -.
DR   EnsemblBacteria; CAB13166; CAB13166; BSU_13090.
DR   GeneID; 936835; -.
DR   KEGG; bsu:BSU13090; -.
DR   PATRIC; fig|224308.179.peg.1421; -.
DR   eggNOG; COG2076; Bacteria.
DR   InParanoid; P49856; -.
DR   OMA; EVFWVIG; -.
DR   PhylomeDB; P49856; -.
DR   BioCyc; BSUB:BSU13090-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR   GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR   InterPro; IPR000390; Small_drug/metabolite_transptr.
DR   InterPro; IPR045324; Small_multidrug_res.
DR   PANTHER; PTHR30561; PTHR30561; 1.
DR   Pfam; PF00893; Multi_Drug_Res; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Ion transport; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..112
FT                   /note="Probable guanidinium efflux system subunit GdnC"
FT                   /id="PRO_0000108111"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        26..46
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        55..75
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   112 AA;  11921 MW;  6D96732F1A280A59 CRC64;
     MKWGLVVLAA VFEVVWVIGL KHADSALTWS GTAIGIIFSF YLLMKATHSL PVGTVYAVFT
     GLGTAGTVLS EIVLFHEPVG WPKLLLIGVL LIGVIGLKLV TQDETEEKGG EA