GDND_BACSU
ID GDND_BACSU Reviewed; 105 AA.
AC P49857; O34690;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Probable guanidinium efflux system subunit GdnD {ECO:0000305};
GN Name=gdnD {ECO:0000312|EMBL:CAB13167.1}; Synonyms=ykkD;
GN OrderedLocusNames=BSU13100;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8682784; DOI=10.1128/jb.178.13.3803-3808.1996;
RA Lacelle M., Kumano M., Kurita K., Yamane K., Zuber P., Nakano M.M.;
RT "Oxygen-controlled regulation of the flavohemoglobin gene in Bacillus
RT subtilis.";
RL J. Bacteriol. 178:3803-3808(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Devine K.M.;
RT "Sequence of the Bacillus subtilis genome between xlyA and ykoR.";
RL Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP FUNCTION, AND SUBUNIT.
RX PubMed=10735877; DOI=10.1128/jb.182.8.2311-2313.2000;
RA Jack D.L., Storms M.L., Tchieu J.H., Paulsen I.T., Saier M.H. Jr.;
RT "A broad-specificity multidrug efflux pump requiring a pair of homologous
RT SMR-type proteins.";
RL J. Bacteriol. 182:2311-2313(2000).
RN [5]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=27989440; DOI=10.1016/j.molcel.2016.11.019;
RA Nelson J.W., Atilho R.M., Sherlock M.E., Stockbridge R.B., Breaker R.R.;
RT "Metabolism of free guanidine in bacteria is regulated by a widespread
RT riboswitch class.";
RL Mol. Cell 65:220-230(2017).
CC -!- FUNCTION: Probably involved in guanidinium transport (PubMed:27989440).
CC In vitro, confers resistance to a broad range of toxic compounds such
CC as cationic dyes, neutral and anionic antimicrobials (PubMed:10735877).
CC {ECO:0000269|PubMed:10735877, ECO:0000269|PubMed:27989440}.
CC -!- SUBUNIT: The efflux pump is composed of GdnC and GdnD.
CC {ECO:0000305|PubMed:10735877}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Transcriptionally regulated by guanidine, via a guanidine-
CC sensing riboswitch. {ECO:0000269|PubMed:27989440}.
CC -!- DISRUPTION PHENOTYPE: Deletion of both gdnC and gdnD leads to a
CC decrease in the concentration of guanidine required to inhibit
CC bacterial growth. {ECO:0000269|PubMed:27989440}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
CC YkkC/YkkD subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA11263.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; D78189; BAA11263.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AJ002571; CAA05589.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13167.1; -; Genomic_DNA.
DR PIR; B69857; B69857.
DR RefSeq; NP_389193.1; NC_000964.3.
DR RefSeq; WP_003245695.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; P49857; -.
DR SMR; P49857; -.
DR STRING; 224308.BSU13100; -.
DR TCDB; 2.A.7.1.5; the drug/metabolite transporter (dmt) superfamily.
DR PaxDb; P49857; -.
DR DNASU; 938109; -.
DR EnsemblBacteria; CAB13167; CAB13167; BSU_13100.
DR GeneID; 938109; -.
DR KEGG; bsu:BSU13100; -.
DR PATRIC; fig|224308.43.peg.1381; -.
DR eggNOG; COG2076; Bacteria.
DR InParanoid; P49857; -.
DR OMA; FEIAWAI; -.
DR PhylomeDB; P49857; -.
DR BioCyc; BSUB:BSU13100-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR InterPro; IPR045324; Small_multidrug_res.
DR PANTHER; PTHR30561; PTHR30561; 1.
DR Pfam; PF00893; Multi_Drug_Res; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..105
FT /note="Probable guanidinium efflux system subunit GdnD"
FT /id="PRO_0000108113"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 105 AA; 11325 MW; 796C69A20142748C CRC64;
MLHWISLLCA GCLEMAGVAL MNQYAKEKSV KWVLLIIVGF AASFSLLSYA METTPMGTAY
AVWTGIGTAG GALIGILFYK EQKDAKRIFF IALILCSAVG LKILS
