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ALP1_YEAST
ID   ALP1_YEAST              Reviewed;         573 AA.
AC   P38971; D6W0S4;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   03-AUG-2022, entry version 161.
DE   RecName: Full=Basic amino-acid permease;
GN   Name=ALP1; OrderedLocusNames=YNL270C; ORFNames=N0660;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 28383 / FL100 / VTT C-80102;
RX   PubMed=7941748; DOI=10.1002/yea.320100509;
RA   Sychrova H., Chevallier M.R.;
RT   "APL1, a yeast gene encoding a putative permease for basic amino acids.";
RL   Yeast 10:653-657(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   PubMed=8740425;
RX   DOI=10.1002/(sici)1097-0061(199604)12:5<505::aid-yea932>3.0.co;2-f;
RA   Sen-Gupta M., Lyck R., Fleig U., Niedenthal R.K., Hegemann J.H.;
RT   "The sequence of a 24,152 bp segment from the left arm of chromosome XIV
RT   from Saccharomyces cerevisiae between the BNI1 and the POL2 genes.";
RL   Yeast 12:505-514(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA   Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA   Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA   Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA   Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA   Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA   Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA   Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA   Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA   Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA   Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA   Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA   Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT   evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   FUNCTION.
RX   PubMed=10654085; DOI=10.1007/s002940050506;
RA   Regenberg B., During-Olsen L., Kielland-Brandt M.C., Holmberg S.;
RT   "Substrate specificity and gene expression of the amino-acid permeases in
RT   Saccharomyces cerevisiae.";
RL   Curr. Genet. 36:317-328(1999).
RN   [6]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC   -!- FUNCTION: High-affinity permease for basic amino acids.
CC       {ECO:0000269|PubMed:10654085}.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC       superfamily. YAT (TC 2.A.3.10) family. {ECO:0000305}.
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DR   EMBL; X74069; CAA52199.1; -; Genomic_DNA.
DR   EMBL; X92494; CAA63228.1; -; Genomic_DNA.
DR   EMBL; Z71546; CAA96177.1; -; Genomic_DNA.
DR   EMBL; BK006947; DAA10290.1; -; Genomic_DNA.
DR   PIR; S60912; S60912.
DR   RefSeq; NP_014129.1; NM_001183108.1.
DR   AlphaFoldDB; P38971; -.
DR   SMR; P38971; -.
DR   BioGRID; 35570; 67.
DR   IntAct; P38971; 1.
DR   STRING; 4932.YNL270C; -.
DR   TCDB; 2.A.3.10.11; the amino acid-polyamine-organocation (apc) family.
DR   PaxDb; P38971; -.
DR   PRIDE; P38971; -.
DR   TopDownProteomics; P38971; -.
DR   EnsemblFungi; YNL270C_mRNA; YNL270C; YNL270C.
DR   GeneID; 855451; -.
DR   KEGG; sce:YNL270C; -.
DR   SGD; S000005214; ALP1.
DR   VEuPathDB; FungiDB:YNL270C; -.
DR   eggNOG; KOG1286; Eukaryota.
DR   GeneTree; ENSGT00940000176760; -.
DR   HOGENOM; CLU_007946_12_1_1; -.
DR   InParanoid; P38971; -.
DR   OMA; RTVIFFI; -.
DR   BioCyc; YEAST:G3O-33264-MON; -.
DR   PRO; PR:P38971; -.
DR   Proteomes; UP000002311; Chromosome XIV.
DR   RNAct; P38971; protein.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR   GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR   GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0015174; F:basic amino acid transmembrane transporter activity; IDA:SGD.
DR   GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR   GO; GO:0015802; P:basic amino acid transport; IDA:SGD.
DR   InterPro; IPR004841; AA-permease/SLC12A_dom.
DR   InterPro; IPR004762; Amino_acid_permease_fungi.
DR   InterPro; IPR004840; Amoino_acid_permease_CS.
DR   Pfam; PF00324; AA_permease; 1.
DR   TIGRFAMs; TIGR00913; 2A0310; 1.
DR   PROSITE; PS00218; AMINO_ACID_PERMEASE_1; 1.
PE   1: Evidence at protein level;
KW   Amino-acid transport; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..573
FT                   /note="Basic amino-acid permease"
FT                   /id="PRO_0000054145"
FT   TOPO_DOM        1..73
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        74..93
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        94..95
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        96..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        117..153
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        154..174
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        175..179
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        180..200
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        201..209
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        210..230
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        231..265
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        266..286
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        287..306
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        307..327
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        328..359
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        360..380
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        381..403
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        404..424
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        425..431
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        432..452
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        453..477
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        478..498
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        499..510
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        511..531
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        532..573
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          40..66
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        42..66
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        51
FT                   /note="D -> DD (in Ref. 1; CAA52199)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        126
FT                   /note="V -> A (in Ref. 1; CAA52199)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        260
FT                   /note="D -> N (in Ref. 1; CAA52199)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        517
FT                   /note="I -> V (in Ref. 1; CAA52199)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        548
FT                   /note="R -> H (in Ref. 1; CAA52199)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   573 AA;  64014 MW;  359DFE1466C348A1 CRC64;
     MDETVNIQMS KEGQYEINSS SIIKEEEFVD EQYSGENVTK AITTERKVED DAAKETESSP
     QERREVKRKL KQRHIGMIAL GGTIGTGLII GIGPPLAHAG PVGALISYLF MGTVIYSVTQ
     SLGEMVTFIP VTSSFSVFAQ RFLSPALGAT NGYMYWLSWC FTFALELSVL GKVIQYWTEA
     VPLAAWIVIF WCLLTSMNMF PVKYYGEFEF CIASIKVIAL LGFIIFSFCV VCGAGQSDGP
     IGFRYWRNPG AWGPGIISSD KNEGRFLGWV SSLINAAFTY QGTELVGITA GEAANPRKAL
     PRAIKKVVVR ILVFYILSLF FIGLLVPYND PKLDSDGIFV SSSPFMISIE NSGTKVLPDI
     FNAVVLITIL SAGNSNVYIG SRVLYSLSKN SLAPRFLSNV TRGGVPYFSV LSTSVFGFLA
     FLEVSAGSGK AFNWLLNITG VAGFFAWLLI SFSHIRFMQA IRKRGISRDD LPYKAQMMPF
     LAYYASFFIA LIVLIQGFTA FAPTFQPIDF VAAYISIFLF LAIWLSFQVW FKCRLLWKLQ
     DIDIDSDRRQ IEELVWIEPE CKTRWQRVWD VLS
 
 
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