ID   ALP21_SCHPO             Reviewed;         511 AA.
AC   Q10303;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   25-MAY-2022, entry version 132.
DE   RecName: Full=Cell polarity protein alp21;
DE   AltName: Full=Altered polarity protein 21;
DE   AltName: Full=Suppressor of tsm one protein 1;
GN   Name=alp21; Synonyms=sto1; ORFNames=SPAC22H10.10;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], DOMAINS LEUCINE-RICH REPEATS, AND
RP   INTERACTION WITH ALP1 AND ALP11.
RX   PubMed=10473641; DOI=10.1091/mbc.10.9.2987;
RA   Radcliffe P.A., Hirata D., Vardy L., Toda T.;
RT   "Functional dissection and hierarchy of tubulin-folding cofactor homologues
RT   in fission yeast.";
RL   Mol. Biol. Cell 10:2987-3001(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=10341216; DOI=10.1242/jcs.112.12.1979;
RA   Grishchuk E.L., McIntosh J.R.;
RT   "Sto1p, a fission yeast protein similar to tubulin folding cofactor E,
RT   plays an essential role in mitotic microtubule assembly.";
RL   J. Cell Sci. 112:1979-1988(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Required for microtubule and spindle assembly. Involved in
CC       the proper folding of alpha-tubulin. {ECO:0000269|PubMed:10341216}.
CC   -!- SUBUNIT: Interacts with alp1 and alp11. {ECO:0000269|PubMed:10473641}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000269|PubMed:10341216}.
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DR   EMBL; AB008749; BAA23374.1; -; Genomic_DNA.
DR   EMBL; CU329670; CAA93613.1; -; Genomic_DNA.
DR   PIR; T43282; T43282.
DR   RefSeq; NP_593747.1; NM_001019178.2.
DR   AlphaFoldDB; Q10303; -.
DR   SMR; Q10303; -.
DR   BioGRID; 278338; 7.
DR   STRING; 4896.SPAC22H10.10.1; -.
DR   MaxQB; Q10303; -.
DR   PaxDb; Q10303; -.
DR   PRIDE; Q10303; -.
DR   EnsemblFungi; SPAC22H10.10.1; SPAC22H10.10.1:pep; SPAC22H10.10.
DR   GeneID; 2541847; -.
DR   KEGG; spo:SPAC22H10.10; -.
DR   PomBase; SPAC22H10.10; alp21.
DR   VEuPathDB; FungiDB:SPAC22H10.10; -.
DR   eggNOG; KOG3207; Eukaryota.
DR   HOGENOM; CLU_584163_0_0_1; -.
DR   InParanoid; Q10303; -.
DR   OMA; VFERTKW; -.
DR   PhylomeDB; Q10303; -.
DR   PRO; PR:Q10303; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IDA:PomBase.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:1990727; C:tubulin folding cofactor complex; NAS:PomBase.
DR   GO; GO:0043014; F:alpha-tubulin binding; IBA:GO_Central.
DR   GO; GO:0051082; F:unfolded protein binding; NAS:PomBase.
DR   GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR   GO; GO:0007023; P:post-chaperonin tubulin folding pathway; IBA:GO_Central.
DR   GO; GO:0007021; P:tubulin complex assembly; IBA:GO_Central.
DR   Gene3D; 3.80.10.10; -; 2.
DR   InterPro; IPR001611; Leu-rich_rpt.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   InterPro; IPR044994; TBCE.
DR   PANTHER; PTHR15140; PTHR15140; 1.
DR   PROSITE; PS51450; LRR; 6.
PE   1: Evidence at protein level;
KW   Chaperone; Cytoplasm; Cytoskeleton; Leucine-rich repeat; Microtubule;
KW   Reference proteome; Repeat.
FT   CHAIN           1..511
FT                   /note="Cell polarity protein alp21"
FT                   /id="PRO_0000064569"
FT   REPEAT          116..137
FT                   /note="LRR 1"
FT   REPEAT          142..163
FT                   /note="LRR 2"
FT   REPEAT          168..190
FT                   /note="LRR 3"
FT   REPEAT          191..217
FT                   /note="LRR 4"
FT   REPEAT          218..241
FT                   /note="LRR 5"
FT   REPEAT          242..264
FT                   /note="LRR 6"
FT   REPEAT          267..288
FT                   /note="LRR 7"
FT   REPEAT          291..312
FT                   /note="LRR 8"
FT   DOMAIN          325..364
FT                   /note="LRRCT"
SQ   SEQUENCE   511 AA;  58591 MW;  B6FD84864AD648B6 CRC64;
     MHISTGMVRA SISNTLVTLT IEGNNDRYQV ILDKNVNAKQ FVKTCWRNKK PLIVKEGKIF
     VDKGFPFDPC RTFLEALYEK YSYSSPLPSS VEFKSGKQVE FCGFEKIQSK QRDLKSLRVI
     ILDNYRIEDI EIEYEYSKIL PEVIDLDLSR NLFHEFFPIL KLCSQLPSLR NLTLDSNLFS
     NFISSNTVLL IPHLTQLSVN GCGLNSKDVQ WITETFPSLE VLYLEANEII LSKATSFKNL
     QFLQTLSLAN NLNLYSADGY AVDVFQGINN LNLSSTSLAD VAELPVHTLH KLTFLDISEN
     NIRDIRSLDH LRTLENLKHL RITLSYFNKP TDIAKLLVIA RIPSLVKLND VNISPNERLD
     AELYYTSCIR KLVIDNEIKD VEELTKIEPF WEEIWKSHEL PSIQFHLEAS INDWTSGILK
     NRITKGIKIS SINSGATMLL KLHYNWKLLD IKALISYHFA IPVHTSTFVF ASSERDVSFS
     PKTVRDDQKR LFELPFTCTF IDVYAKESGN V