GDT1_DICDI
ID GDT1_DICDI Reviewed; 1661 AA.
AC Q54XS3; Q9GRX5; Q9U987;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Probable inactive serine/threonine-protein kinase gdt1;
DE AltName: Full=Growth-differentiation transition protein 1;
DE Flags: Precursor;
GN Name=gdt1; ORFNames=DDB_G0278723;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 102-1661, FUNCTION, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=AX2;
RX PubMed=10793140; DOI=10.1091/mbc.11.5.1631;
RA Zeng C., Anjard C., Riemann K., Konzok A., Nellen W.;
RT "gdt1, a new signal transduction component for negative regulation of the
RT growth-differentiation transition.";
RL Mol. Biol. Cell 11:1631-1643(2000).
RN [3]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=15236669; DOI=10.1186/1471-213x-4-8;
RA Chibalina M.V., Anjard C., Insall R.H.;
RT "Gdt2 regulates the transition of Dictyostelium cells from growth to
RT differentiation.";
RL BMC Dev. Biol. 4:8-8(2004).
CC -!- FUNCTION: Regulates the transition between growth and differentiation.
CC {ECO:0000269|PubMed:10793140}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed in vegetative cells only (at protein
CC level). {ECO:0000269|PubMed:10793140}.
CC -!- DOMAIN: The protein kinase domain is predicted to be catalytically
CC inactive.
CC -!- DISRUPTION PHENOTYPE: Cells overexpress discoidin and initiate
CC multicellular development prematurely. {ECO:0000269|PubMed:10793140}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the GDT family.
CC {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the protein kinase
CC superfamily. TKL Ser/Thr protein kinase family. {ECO:0000305}.
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DR EMBL; AAFI02000024; EAL67963.2; -; Genomic_DNA.
DR EMBL; AJ000992; CAB51908.1; -; Genomic_DNA.
DR EMBL; AJ279060; CAC09932.1; -; Genomic_DNA.
DR RefSeq; XP_641957.2; XM_636865.2.
DR AlphaFoldDB; Q54XS3; -.
DR SMR; Q54XS3; -.
DR STRING; 44689.DDB0215003; -.
DR PaxDb; Q54XS3; -.
DR PRIDE; Q54XS3; -.
DR EnsemblProtists; EAL67963; EAL67963; DDB_G0278723.
DR GeneID; 8621689; -.
DR KEGG; ddi:DDB_G0278723; -.
DR dictyBase; DDB_G0278723; gdt1.
DR eggNOG; KOG0192; Eukaryota.
DR HOGENOM; CLU_251247_0_0_1; -.
DR InParanoid; Q54XS3; -.
DR PhylomeDB; Q54XS3; -.
DR PRO; PR:Q54XS3; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004672; F:protein kinase activity; IEA:InterPro.
DR GO; GO:0051093; P:negative regulation of developmental process; IMP:dictyBase.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR GO; GO:0050793; P:regulation of developmental process; IBA:GO_Central.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR026237; STKINASEGDT.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR PRINTS; PR02079; STKINASEGDT.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Developmental protein; Membrane; Nucleotide-binding;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..1661
FT /note="Probable inactive serine/threonine-protein kinase
FT gdt1"
FT /id="PRO_0000323579"
FT TOPO_DOM 21..1007
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1008..1028
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1029..1661
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 1372..1661
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 802..827
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1089..1175
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1547..1584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 1378..1386
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 1399
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT CONFLICT 121
FT /note="I -> V (in Ref. 2; CAB51908)"
FT /evidence="ECO:0000305"
FT CONFLICT 720
FT /note="F -> S (in Ref. 2; CAB51908)"
FT /evidence="ECO:0000305"
FT CONFLICT 1243..1251
FT /note="ALSDFKDFS -> RHYQILKIFP (in Ref. 2; CAB51908)"
FT /evidence="ECO:0000305"
FT CONFLICT 1591
FT /note="I -> N (in Ref. 2; CAB51908)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1661 AA; 186642 MW; B6A5F41CD9018B83 CRC64;
MKKILLSIFF VVFLLIGSEC ELIDTPPGYY NLKKIDKFQQ FSKIQIERNN KTIYSSSFHD
LFYPDICEGT IQNIPGSLYF GRSSFPGAQE SSNLMITRGT KMKYRVGNGG IPIYKINVLC
IEGTLEIPEG ISFFNVGALF ILPGGVLNSK SSIRFTDLDP YNSKMDPFNF FPGMMVLGGS
LSLIGEKKRI FQATRIDDYQ LQIEDFKKIG SLTNNIYLGS KVTIYSQQIS EGQTCSFSFG
ASNDKINLTS SSSSSLRGTN CLPISKNDKN IIVHFNCKYL LGGSSESFID STSTVGSSIY
ITGDSQVQIE NFTLDSIGKT TNKLYNDTKL IFSNDKPNQV IDIIKGENQR FRNSLYIEFS
NSVVIKGCAI IDRVKESRAP LIFVSSNVSL SESLIVSKSG SNLIAQYGTE FIKSKLNHYF
LIPPLPQPFG LNSPINFPSP FSMDYGFEGN GIYSLSPNVQ SINDTFISQL IALNFNFIGN
RSIITGFDND CYSPCTNTSI LFSNLIQYPV DFKINNSTYF YKLNNNNNDN NNNTNNFNLL
NINNENNNND DNNNNNNSQN HYLLNINNNG NSPGRFFTIK DLVASDTVSA YLPNSALVFN
NLNATENFSF NGTVSRLDFI NSSFNTNLTS TQKFFDETTT TVTNFQNTYI YGSPETTEPP
EKVFGSSITP IYYFSKSVLD SLKVETVYPN EPHKMFFNSS VSLSVKLNNM GLKTPIICIF
SSNGMESKEV QYNPISTKCS FLYVSEKLGN HNIRVTIKNE YSKDSSYYFI IDFPIITVYG
QSLFNAGWQM IDPTSTTIIP TPTPTSTSTS TSTSTPTTTN NNQLVNDNNN KINELDGLKF
QGGCIKTLGC QLSSNAKYVG GLPNVTASST LNSLFSWGIT SDVQYDPVII DLFINKSIAT
LQVQLFFTFY KPIDQYSSPL SVSIQKNPVL VMEPFAGDQP FSKNLTFVYN NTQSLDFLNI
SFTSRGDIYL TSLAIFSVSD SLPQIIDPIT PTLLPIESVK ASKPAILAIV LSIVLGSLAL
SIITILIVKH RKRLSQFLSK SNKDIEYAQN NEIEIKVLPK ITSHSSYPSI SILDTISSDS
IFNNQIPKNN NRYKFKNQSL NNNNYFNNNN NNNNSNNNNS NNIIYSNCNS NYSNSNSNNN
NNNNNSNSNN NSNSNSNINI NSNSNSNSNS NSNGNNNYQI YSNKLESFKI DEISNDTIPI
INSTFPDEFQ TLEFQKLAFE ILKREKRLDF SFRTTNDILT CCALSDFKDF SNFPLRFNQS
IITFGLINGK AKLGETYYDT LSITNDSTIR FTAFLILPMD NHSATFTSDH SSFDLGPGET
FSIKFSITLH CTTRFFENFS IQINSNNIKE MYTLLKIKVE SESSTRLDFN DIHFQELIEK
YSWEILYRGT VGDKNALLKL IKLKTKNCEE AYRELNIISR LKHQNILPLI GCVISKDYLC
LAFEYPPLGS LDYIISKKKL KMSITQKIRI LIDVAKGCKF LQQSSIIQKT LRARNIFLYD
TNENAEVCAK VLDLTSSKTI KGLACNNYIE RVDTPINLTR EISIIRDPKQ NNDFNNSNNS
NNNNNNNNNN NNNNNNNSNN SNNSSSLKYN IHSFAVLSYE LLIDEILVGD TRKFGQEKPS
IGLDKIDPNI KNFIHKCWNP IDGFTFNEIL KTLKDFIESL N
