GDT1_YEAST
ID GDT1_YEAST Reviewed; 280 AA.
AC P38301; D6VQI2; E9PAD4; Q6Q545;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=GCR1-dependent translation factor 1;
GN Name=GDT1; OrderedLocusNames=YBR187W; ORFNames=YBR1310;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7871891; DOI=10.1002/yea.320101116;
RA Demolis N., Jacquet M., Mallet L.;
RT "A 12.5 kb fragment of the yeast chromosome II contains two adjacent genes
RT encoding ribosomal proteins and six putative new genes, one of which
RT encodes a putative transcriptional factor.";
RL Yeast 10:1511-1525(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RA Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E.,
RA Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., Zuo D.,
RA Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., Gonzaga L.,
RA Vasconcelos A.T., Simpson A.J.G., Kolodner R., Harlow E., LaBaer J.;
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [8]
RP INDUCTION.
RX PubMed=17124610; DOI=10.1007/s00438-006-0182-0;
RA Barbara K.E., Haley T.M., Willis K.A., Santangelo G.M.;
RT "The transcription factor Gcr1 stimulates cell growth by participating in
RT nutrient-responsive gene expression on a global level.";
RL Mol. Genet. Genomics 277:171-188(2007).
RN [9]
RP MUTAGENESIS OF ARG-71.
RX PubMed=23575229; DOI=10.1093/hmg/ddt146;
RA Rosnoblet C., Legrand D., Demaegd D., Hacine-Gherbi H., de Bettignies G.,
RA Bammens R., Borrego C., Duvet S., Morsomme P., Matthijs G., Foulquier F.;
RT "Impact of disease-causing mutations on TMEM165 subcellular localization, a
RT recently identified protein involved in CDG-II.";
RL Hum. Mol. Genet. 22:2914-2928(2013).
RN [10]
RP FUNCTION IN CALCIUM HOMEOSTASIS, AND SUBCELLULAR LOCATION.
RX PubMed=23569283; DOI=10.1073/pnas.1219871110;
RA Demaegd D., Foulquier F., Colinet A.S., Gremillon L., Legrand D.,
RA Mariot P., Peiter E., Van Schaftingen E., Matthijs G., Morsomme P.;
RT "Newly characterized Golgi-localized family of proteins is involved in
RT calcium and pH homeostasis in yeast and human cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:6859-6864(2013).
CC -!- FUNCTION: May be involved in ribosome biogenesis. Has also been
CC proposed to play a role in calcium homeostasis (PubMed:23569283).
CC {ECO:0000269|PubMed:23569283}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane; Multi-pass membrane protein.
CC Golgi apparatus membrane.
CC -!- INDUCTION: Expression is GCR1-dependent. Promoter is also bound by the
CC RAP1 transcription factor. {ECO:0000269|PubMed:17124610}.
CC -!- MISCELLANEOUS: Present with 2170 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the GDT1 family. {ECO:0000305}.
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DR EMBL; U02073; AAB60282.1; -; Genomic_DNA.
DR EMBL; Z36056; CAA85148.1; -; Genomic_DNA.
DR EMBL; Z36057; CAA85150.1; -; Genomic_DNA.
DR EMBL; AY558567; AAS56893.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07302.1; -; Genomic_DNA.
DR PIR; S46059; S46059.
DR RefSeq; NP_009746.1; NM_001178535.1.
DR AlphaFoldDB; P38301; -.
DR BioGRID; 32885; 84.
DR DIP; DIP-3804N; -.
DR IntAct; P38301; 3.
DR MINT; P38301; -.
DR STRING; 4932.YBR187W; -.
DR TCDB; 2.A.106.2.3; the ca(2+):h(+) antiporter-2 (caca2) family.
DR iPTMnet; P38301; -.
DR MaxQB; P38301; -.
DR PaxDb; P38301; -.
DR PRIDE; P38301; -.
DR EnsemblFungi; YBR187W_mRNA; YBR187W; YBR187W.
DR GeneID; 852485; -.
DR KEGG; sce:YBR187W; -.
DR SGD; S000000391; GDT1.
DR VEuPathDB; FungiDB:YBR187W; -.
DR eggNOG; KOG2881; Eukaryota.
DR GeneTree; ENSGT00390000005261; -.
DR HOGENOM; CLU_040186_0_0_1; -.
DR InParanoid; P38301; -.
DR OMA; QGKWHSF; -.
DR BioCyc; YEAST:G3O-29130-MON; -.
DR PRO; PR:P38301; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38301; protein.
DR GO; GO:0005801; C:cis-Golgi network; IDA:SGD.
DR GO; GO:0000324; C:fungal-type vacuole; IDA:SGD.
DR GO; GO:0000329; C:fungal-type vacuole membrane; HDA:SGD.
DR GO; GO:0005794; C:Golgi apparatus; IDA:UniProtKB.
DR GO; GO:0005797; C:Golgi medial cisterna; IDA:SGD.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015085; F:calcium ion transmembrane transporter activity; IDA:SGD.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IDA:SGD.
DR GO; GO:0046873; F:metal ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IDA:SGD.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IMP:UniProtKB.
DR GO; GO:0030026; P:cellular manganese ion homeostasis; IMP:SGD.
DR GO; GO:0032468; P:Golgi calcium ion homeostasis; IMP:SGD.
DR GO; GO:0032472; P:Golgi calcium ion transport; IBA:GO_Central.
DR GO; GO:0071421; P:manganese ion transmembrane transport; IDA:SGD.
DR InterPro; IPR001727; Gdt1.
DR PANTHER; PTHR12608; PTHR12608; 1.
DR Pfam; PF01169; UPF0016; 2.
DR PROSITE; PS01214; UPF0016; 1.
PE 1: Evidence at protein level;
KW Golgi apparatus; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Vacuole.
FT CHAIN 1..280
FT /note="GCR1-dependent translation factor 1"
FT /id="PRO_0000212467"
FT TOPO_DOM 1..3
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 25..45
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..71
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 93..104
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 126..183
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 205..222
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 244..255
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..280
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT MUTAGEN 71
FT /note="R->C,H: Loss of function in calcium homeostasis."
FT /evidence="ECO:0000269|PubMed:23575229"
FT CONFLICT 158
FT /note="K -> E (in Ref. 4; AAS56893)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 280 AA; 30341 MW; 34CDAE95E13169FE CRC64;
MGNMIKKASL IALLPLFTAA AAAATDAETS MESGSSSHLK SFLMSVSMIG LSEIGDKTFL
IAALMAMRHK RVLVFSAAAT SLAIMTILSG VVGHSAVAFL SERYTAFFAG ILFLVFGYKL
TMEGLEMSKD AGVEEEMAEV EEEIAIKDMN QDMDDVEKGG DTAYDKQLKN ASIGKKIVHR
IRELASFMFS PVWVQIFLMV FLGELGDRSQ ISIIAMATDS DYWYVIAGAV IGHAICSGLA
VVGGKLLATR ISIRTITLAS SLLFFIFALM YIYQAFTTQD
