ID   GDU5_ARATH              Reviewed;         131 AA.
AC   Q3E965;
DT   31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 2.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=Protein GLUTAMINE DUMPER 5;
GN   Name=GDU5; OrderedLocusNames=At5g24920; ORFNames=F6A4.130;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130714; DOI=10.1038/35048507;
RA   Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA   Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA   Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA   Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA   O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA   Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA   Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA   Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA   Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA   Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA   Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA   Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA   Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA   Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA   Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA   McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA   Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA   Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA   Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA   Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA   Bevan M., Fransz P.F.;
RT   "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL   Nature 408:823-826(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY.
RX   PubMed=15208395; DOI=10.1105/tpc.021642;
RA   Pilot G., Stransky H., Bushey D.F., Pratelli R., Ludewig U., Wingate V.P.,
RA   Frommer W.B.;
RT   "Overexpression of GLUTAMINE DUMPER1 leads to hypersecretion of glutamine
RT   from hydathodes of Arabidopsis leaves.";
RL   Plant Cell 16:1827-1840(2004).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=17157837; DOI=10.1016/j.febslet.2006.11.064;
RA   Pratelli R., Pilot G.;
RT   "The plant-specific VIMAG domain of Glutamine Dumper1 is necessary for the
RT   function of the protein in Arabidopsis.";
RL   FEBS Lett. 580:6961-6966(2006).
RN   [5]
RP   ERRATUM OF PUBMED:17157837.
RX   DOI=10.1016/j.febslet.2007.02.027;
RA   Pratelli R., Pilot G.;
RL   FEBS Lett. 581:1248-1249(2007).
RN   [6]
RP   FUNCTION, TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RA   Pratelli R., Frommer W.B., Pilot G.;
RT   "The over-expression of GDU-like genes leads to modification in amino acid
RT   content and transport.";
RL   (In) Proceedings of the 19th international conference on Arabidopsis
RL   research, pp.abstract#10018, Montreal (2008).
RN   [7]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=20018597; DOI=10.1104/pp.109.151746;
RA   Pratelli R., Voll L.M., Horst R.J., Frommer W.B., Pilot G.;
RT   "Stimulation of nonselective amino acid export by glutamine dumper
RT   proteins.";
RL   Plant Physiol. 152:762-773(2010).
CC   -!- FUNCTION: Probable subunit of an amino acid transporter involved in the
CC       regulation of the amino acid metabolism. Stimulates amino acid export
CC       by activating nonselective amino acid facilitators.
CC       {ECO:0000269|PubMed:20018597, ECO:0000269|Ref.6}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|Ref.6}; Single-pass
CC       membrane protein {ECO:0000269|Ref.6}.
CC   -!- TISSUE SPECIFICITY: Expressed in the vascular tissues. Also detected in
CC       guard cells. {ECO:0000269|PubMed:20018597, ECO:0000269|Ref.6}.
CC   -!- DOMAIN: The VIMAG motif is necessary for the function of the protein.
CC       {ECO:0000250}.
CC   -!- MISCELLANEOUS: Overexpression of GLUTAMINE DUMPER 5 leads to free amino
CC       acid levels accumulation and plant size decrease (Ref.6,
CC       PubMed:20018597).
CC   -!- SIMILARITY: Belongs to the GLUTAMINE DUMPER 1 (TC 9.B.60) family.
CC       {ECO:0000305}.
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DR   EMBL; AF069716; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CP002688; AED93378.1; -; Genomic_DNA.
DR   RefSeq; NP_197874.2; NM_122401.3.
DR   AlphaFoldDB; Q3E965; -.
DR   SMR; Q3E965; -.
DR   BioGRID; 17837; 11.
DR   IntAct; Q3E965; 11.
DR   MINT; Q3E965; -.
DR   STRING; 3702.AT5G24920.1; -.
DR   PaxDb; Q3E965; -.
DR   PRIDE; Q3E965; -.
DR   ProteomicsDB; 247127; -.
DR   EnsemblPlants; AT5G24920.1; AT5G24920.1; AT5G24920.
DR   GeneID; 832562; -.
DR   Gramene; AT5G24920.1; AT5G24920.1; AT5G24920.
DR   KEGG; ath:AT5G24920; -.
DR   Araport; AT5G24920; -.
DR   TAIR; locus:2149448; AT5G24920.
DR   eggNOG; ENOG502S4AK; Eukaryota.
DR   HOGENOM; CLU_112624_2_3_1; -.
DR   InParanoid; Q3E965; -.
DR   OMA; KICLDCV; -.
DR   OrthoDB; 1520115at2759; -.
DR   PhylomeDB; Q3E965; -.
DR   PRO; PR:Q3E965; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q3E965; baseline and differential.
DR   GO; GO:0016021; C:integral component of membrane; ISS:TAIR.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   GO; GO:0080143; P:regulation of amino acid export; IMP:TAIR.
DR   InterPro; IPR040359; GDU.
DR   PANTHER; PTHR33228; PTHR33228; 1.
PE   2: Evidence at transcript level;
KW   Amino-acid transport; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..131
FT                   /note="Protein GLUTAMINE DUMPER 5"
FT                   /id="PRO_0000419943"
FT   TOPO_DOM        1..34
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        35..55
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        56..131
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   MOTIF           88..92
FT                   /note="VIMAG"
SQ   SEQUENCE   131 AA;  14684 MW;  F492ED829E802DE1 CRC64;
     MRQFPSIRGN INEKMMTTMV ESQTRSPWRT PVPYLFGGLA AMLGLIAFAL LLLACSYWRL
     SRQTEDEEKQ TESGEKVVAK AFEEKILVIM AGQNNPTFLA TPVAAKICLD CVNMEKKEGQ
     NGESKVTEEN H