ALPA_ECOLI
ID ALPA_ECOLI Reviewed; 70 AA.
AC P33997; Q2MAE7;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 25-MAY-2022, entry version 133.
DE RecName: Full=DNA-binding transcriptional activator AlpA {ECO:0000305};
DE AltName: Full=Prophage CP4-57 regulatory protein AlpA;
GN Name=alpA {ECO:0000303|PubMed:7511582}; Synonyms=alp;
GN OrderedLocusNames=b2624, JW2604;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=K12;
RX PubMed=7511582; DOI=10.1128/jb.176.7.2061-2067.1994;
RA Trempy J.E., Kirby J.E., Gottesman S.;
RT "Alp suppression of Lon: dependence on the slpA gene.";
RL J. Bacteriol. 176:2061-2067(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION.
RC STRAIN=K12;
RX PubMed=7511583; DOI=10.1128/jb.176.7.2068-2081.1994;
RA Kirby J.E., Trempy J.E., Gottesman S.;
RT "Excision of a P4-like cryptic prophage leads to Alp protease expression in
RT Escherichia coli.";
RL J. Bacteriol. 176:2068-2081(1994).
CC -!- FUNCTION: Positive regulator of the expression of the slpA gene
CC (PubMed:7511582). When overexpressed, leads to suppression of the
CC capsule overproduction and UV sensitivity phenotypes of cells mutant
CC for the Lon ATP-dependent protease (PubMed:7511582). Part of the
CC cryptic P4-like prophage CP4-57 (PubMed:7511583). Overexpression of
CC AlpA leads to excision of the CP4-57 prophage by IntA. This inactivates
CC ssrA (the gene upstream of the prophage) that encodes tmRNA which is
CC required to rescue stalled ribosomes in a process known as trans-
CC translation (PubMed:7511583). {ECO:0000269|PubMed:7511582,
CC ECO:0000269|PubMed:7511583}.
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DR EMBL; U03737; AAA18418.1; -; Unassigned_DNA.
DR EMBL; U36840; AAA79793.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75672.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76759.1; -; Genomic_DNA.
DR PIR; B65041; B65041.
DR RefSeq; NP_417113.1; NC_000913.3.
DR RefSeq; WP_001065343.1; NZ_LN832404.1.
DR AlphaFoldDB; P33997; -.
DR BioGRID; 4260621; 8.
DR IntAct; P33997; 3.
DR STRING; 511145.b2624; -.
DR PaxDb; P33997; -.
DR PRIDE; P33997; -.
DR EnsemblBacteria; AAC75672; AAC75672; b2624.
DR EnsemblBacteria; BAE76759; BAE76759; BAE76759.
DR GeneID; 946758; -.
DR KEGG; ecj:JW2604; -.
DR KEGG; eco:b2624; -.
DR PATRIC; fig|1411691.4.peg.4115; -.
DR EchoBASE; EB2035; -.
DR eggNOG; COG3311; Bacteria.
DR HOGENOM; CLU_140176_15_0_6; -.
DR InParanoid; P33997; -.
DR OMA; EIAEWIN; -.
DR PhylomeDB; P33997; -.
DR BioCyc; EcoCyc:PD02850; -.
DR PRO; PR:P33997; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:EcoCyc.
DR GO; GO:0044010; P:single-species biofilm formation; IEP:EcoCyc.
DR InterPro; IPR010260; AlpA.
DR Pfam; PF05930; Phage_AlpA; 1.
PE 4: Predicted;
KW Activator; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..70
FT /note="DNA-binding transcriptional activator AlpA"
FT /id="PRO_0000064571"
FT DNA_BIND 12..31
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
SQ SEQUENCE 70 AA; 8133 MW; 85E3E99885A1EF4C CRC64;
MNNRSAVRIL RLPAVIQKTG MARATIYDWL NPKSPRYDAT FPKKRMLGVK SVGWIEAEID
EWLSQRCKLI
