GEF1_SCHPO
ID GEF1_SCHPO Reviewed; 753 AA.
AC Q09763;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Rho guanine nucleotide exchange factor gef1;
GN Name=gef1; ORFNames=SPAC24H6.09;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION, INTERACTION WITH CDC42, AND SUBCELLULAR LOCATION.
RX PubMed=12529446; DOI=10.1091/mbc.e02-07-0400;
RA Coll P.M., Trillo Y., Ametzazurra A., Perez P.;
RT "Gef1p, a new guanine nucleotide exchange factor for Cdc42p, regulates
RT polarity in Schizosaccharomyces pombe.";
RL Mol. Biol. Cell 14:313-323(2003).
RN [3]
RP FUNCTION, AND INTERACTION WITH CDC42.
RX PubMed=12972551; DOI=10.1091/mbc.e02-10-0665;
RA Hirota K., Tanaka K., Ohta K., Yamamoto M.;
RT "Gef1p and Scd1p, the Two GDP-GTP exchange factors for Cdc42p, form a ring
RT structure that shrinks during cytokinesis in Schizosaccharomyces pombe.";
RL Mol. Biol. Cell 14:3617-3627(2003).
CC -!- FUNCTION: Has a role in the control of cell polarity and cytokinesis.
CC Involved in bipolar growth, via modulation of cdc42-shk1-orb6
CC signaling, and septum formation. Stimulates guanine nucleotide exchange
CC of cdc42. {ECO:0000269|PubMed:12529446, ECO:0000269|PubMed:12972551}.
CC -!- SUBUNIT: Interacts with cdc42. {ECO:0000269|PubMed:12529446,
CC ECO:0000269|PubMed:12972551}.
CC -!- INTERACTION:
CC Q09763; Q01112: cdc42; NbExp=7; IntAct=EBI-1556299, EBI-767502;
CC Q09763; Q9UUM7: hob3; NbExp=5; IntAct=EBI-1556299, EBI-1556284;
CC -!- SUBCELLULAR LOCATION: Cell septum {ECO:0000269|PubMed:12529446}.
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DR EMBL; CU329670; CAA90853.1; -; Genomic_DNA.
DR PIR; T38365; S62411.
DR RefSeq; NP_592943.1; NM_001018344.2.
DR AlphaFoldDB; Q09763; -.
DR SMR; Q09763; -.
DR BioGRID; 279089; 48.
DR IntAct; Q09763; 2.
DR MINT; Q09763; -.
DR STRING; 4896.SPAC24H6.09.1; -.
DR iPTMnet; Q09763; -.
DR MaxQB; Q09763; -.
DR PaxDb; Q09763; -.
DR PRIDE; Q09763; -.
DR EnsemblFungi; SPAC24H6.09.1; SPAC24H6.09.1:pep; SPAC24H6.09.
DR GeneID; 2542635; -.
DR KEGG; spo:SPAC24H6.09; -.
DR PomBase; SPAC24H6.09; gef1.
DR VEuPathDB; FungiDB:SPAC24H6.09; -.
DR eggNOG; KOG3519; Eukaryota.
DR HOGENOM; CLU_369673_0_0_1; -.
DR InParanoid; Q09763; -.
DR OMA; KWREKPA; -.
DR PhylomeDB; Q09763; -.
DR Reactome; R-SPO-416482; G alpha (12/13) signalling events.
DR Reactome; R-SPO-9013148; CDC42 GTPase cycle.
DR PRO; PR:Q09763; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0051285; C:cell cortex of cell tip; IDA:PomBase.
DR GO; GO:1902716; C:cell cortex of growing cell tip; IDA:PomBase.
DR GO; GO:0032153; C:cell division site; IDA:PomBase.
DR GO; GO:0051286; C:cell tip; IDA:PomBase.
DR GO; GO:0005737; C:cytoplasm; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0000935; C:division septum; IDA:PomBase.
DR GO; GO:0061645; C:endocytic patch; IDA:PomBase.
DR GO; GO:0031097; C:medial cortex; IDA:PomBase.
DR GO; GO:0110085; C:mitotic actomyosin contractile ring; IDA:PomBase.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:PomBase.
DR GO; GO:0051519; P:activation of bipolar cell growth; IMP:PomBase.
DR GO; GO:0000917; P:division septum assembly; IEA:UniProtKB-KW.
DR GO; GO:0045806; P:negative regulation of endocytosis; IMP:PomBase.
DR GO; GO:2000784; P:positive regulation of establishment of cell polarity regulating cell shape; EXP:PomBase.
DR GO; GO:1903473; P:positive regulation of mitotic actomyosin contractile ring contraction; IMP:PomBase.
DR GO; GO:0032955; P:regulation of division septum assembly; IMP:PomBase.
DR GO; GO:2000100; P:regulation of establishment or maintenance of bipolar cell polarity regulating cell shape; IMP:PomBase.
DR GO; GO:1901901; P:regulation of protein localization to cell division site involved in cytokinesis; IMP:PomBase.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 1.20.900.10; -; 1.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR Pfam; PF00621; RhoGEF; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SUPFAM; SSF48065; SSF48065; 1.
DR PROSITE; PS50010; DH_2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Guanine-nucleotide releasing factor;
KW Reference proteome; Septation.
FT CHAIN 1..753
FT /note="Rho guanine nucleotide exchange factor gef1"
FT /id="PRO_0000080988"
FT DOMAIN 311..507
FT /note="DH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00062"
FT REGION 52..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 175..194
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 200..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 753 AA; 84486 MW; 5740A1AE0D67F207 CRC64;
METLKADLSD MSDSPEYFET LANRDLPRLP GTSKLHRAAC IKRKSINLSL PSNSYSLSYR
QSDDTDGDVS ESQSEYRLSS GRRSRASFAR ALQDPQTPNT PPVSSQHRRF FSEGSFNLPN
SNMSHSLNGD STASNSSTLT PNRIYGDRNR QDYAQSSRYT LPSLPSSPSY NCPTTLRKIH
TNTSSNGTSR RVSGLGSFMA QNSSETSSNR TSAYLPGSST DEQEKRSSVT LASMPSSHSS
TASLLSPLDT TSFSTKLDST ILALETDESL SRTISYATTS LPSTPGKRLS KESLAMSEAS
SINPEYKRIK KRANLIKELV TTEAAYLNDL IAIQQSYGLR VKECSALNPV DAQTVFGDIE
SLLTFTVEFH SRLYQAGEGS WRVNLDTQLI DPLPCNLGLI FLESLSEIGQ IYTGYCNRQD
SVFKIITKWR EKPATASWIM EGDKIVQKYT NAWDLGSLII KPLQRLLKYP LLLQKIIDVT
PESSSERPDL VLSYQLLQEL ISGINQKQKP SHKRGSLSAS HKRDAAWSLL YKATSNKSRP
TTTSTELKTD ARLNFQRQVL QDFRQRFAIL KALHATLETW YVTVHRGFSV FEKVLAELEG
LSALEPEDKP VDTWRKYHLL AHMMTANLPS QIQTSLNSSI LNPITNILRV IQKVIQFIIS
AEFVIPAQKL EAISTLVEKE FHSVVYHFIG IQRSLYENYA QGFLFLIPQD MRDSILEETQ
DYAELVRAFE PMHYDDEVLL EELMKSVSLA ARV
