ID   GEF3_SCHPO              Reviewed;         525 AA.
AC   O59679;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Rho guanine nucleotide exchange factor gef3;
GN   Name=gef3; ORFNames=SPBC29A3.17;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   FUNCTION.
RX   PubMed=14637153; DOI=10.1016/j.bbrc.2003.10.140;
RA   Iwaki N., Karatsu K., Miyamoto M.;
RT   "Role of guanine nucleotide exchange factors for Rho family GTPases in the
RT   regulation of cell morphology and actin cytoskeleton in fission yeast.";
RL   Biochem. Biophys. Res. Commun. 312:414-420(2003).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- FUNCTION: Has a role in the control of cell polarity and cytokinesis.
CC       Involved in bipolar growth and septum formation.
CC       {ECO:0000269|PubMed:14637153}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}.
CC       Note=Septum.
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DR   EMBL; CU329671; CAA18394.1; -; Genomic_DNA.
DR   PIR; T40088; T40088.
DR   RefSeq; NP_595845.1; NM_001021749.2.
DR   AlphaFoldDB; O59679; -.
DR   SMR; O59679; -.
DR   BioGRID; 276864; 12.
DR   STRING; 4896.SPBC29A3.17.1; -.
DR   iPTMnet; O59679; -.
DR   MaxQB; O59679; -.
DR   PaxDb; O59679; -.
DR   EnsemblFungi; SPBC29A3.17.1; SPBC29A3.17.1:pep; SPBC29A3.17.
DR   GeneID; 2540334; -.
DR   KEGG; spo:SPBC29A3.17; -.
DR   PomBase; SPBC29A3.17; gef3.
DR   VEuPathDB; FungiDB:SPBC29A3.17; -.
DR   eggNOG; KOG3519; Eukaryota.
DR   HOGENOM; CLU_524923_0_0_1; -.
DR   InParanoid; O59679; -.
DR   OMA; SMAKIES; -.
DR   PhylomeDB; O59679; -.
DR   Reactome; R-SPO-416482; G alpha (12/13) signalling events.
DR   Reactome; R-SPO-9013148; CDC42 GTPase cycle.
DR   PRO; PR:O59679; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0032153; C:cell division site; HDA:PomBase.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0036391; C:medial cortex septin ring; IDA:PomBase.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:PomBase.
DR   GO; GO:0000917; P:division septum assembly; IEA:UniProtKB-KW.
DR   GO; GO:2001043; P:positive regulation of septum digestion after cytokinesis; EXP:PomBase.
DR   GO; GO:1905391; P:regulation of protein localization to cell division site involved in cell separation after cytokinesis; IMP:PomBase.
DR   GO; GO:0016192; P:vesicle-mediated transport; IMP:PomBase.
DR   CDD; cd00160; RhoGEF; 1.
DR   Gene3D; 1.20.1270.60; -; 1.
DR   Gene3D; 1.20.900.10; -; 1.
DR   InterPro; IPR027267; AH/BAR_dom_sf.
DR   InterPro; IPR035899; DBL_dom_sf.
DR   InterPro; IPR000219; DH-domain.
DR   Pfam; PF00621; RhoGEF; 1.
DR   SMART; SM00325; RhoGEF; 1.
DR   SUPFAM; SSF103657; SSF103657; 1.
DR   SUPFAM; SSF48065; SSF48065; 1.
DR   PROSITE; PS50010; DH_2; 1.
PE   4: Predicted;
KW   Cell cycle; Cell division; Cytoplasm; Guanine-nucleotide releasing factor;
KW   Reference proteome; Septation.
FT   CHAIN           1..525
FT                   /note="Rho guanine nucleotide exchange factor gef3"
FT                   /id="PRO_0000255583"
FT   DOMAIN          72..268
FT                   /note="DH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00062"
SQ   SEQUENCE   525 AA;  60280 MW;  F2422E130EE338BD CRC64;
     MSNPRMFQSR GKRVVSDSLP IANSTHTLED TLKLRPDWTI ALSQHETETR STRTSCSTTT
     ESTIAMRSKQ KAIISVLEEF RDSEAAYVHD LHVAQRYYAD RLSDRVKKSE WKDVFEIFLV
     LCKQASLFEI EMHKSLNDEI NYILDDQDAC LKPKPSVAQL FLSWLPKLSA VYGRYCVIQE
     NIGKKVEKWM KNSSISEYLQ ECDSMAKIES NSWNLDSFLV KPVQRFLKYP LLLNQLYRSA
     SLGIISDYVL LGEACHKSEI ASQRMNELKR RRDIIITALD SVSNSQEVLL LSTDSIDKKI
     AKLQNSTNIF YVPEHEPILA FVHQLSSSYT NLLNLRSAIC DWLKFSRYHY LKFFTFVEAY
     SVFCKDTKSA DKWALISVAL DNIAKGAVLR LTEQCQTSVL RPISNGILFF RNPLCVTDVW
     IKKATAFSKR RQSQVFEEDL ESFPLLSNCL LEELPLFLEM ARNVTDECIL AFAQIQATFY
     DTIQKVLEPV VAKFNLTDHQ DIPSIESIMD FTSLRSSMES SPKSK