GEFC_DICDI
ID GEFC_DICDI Reviewed; 1457 AA.
AC Q8IS20; Q54RZ8;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=Ras guanine nucleotide exchange factor C;
DE AltName: Full=RasGEF domain-containing protein C;
GN Name=gefC; Synonyms=racGEF, rasGEF; ORFNames=DDB_G0282381;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AX4;
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION.
RX PubMed=17380187; DOI=10.1038/sj.embor.7400936;
RA Kae H., Kortholt A., Rehmann H., Insall R.H., Van Haastert P.J.,
RA Spiegelman G.B., Weeks G.;
RT "Cyclic AMP signalling in Dictyostelium: G-proteins activate separate Ras
RT pathways using specific RasGEFs.";
RL EMBO Rep. 8:477-482(2007).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP.
CC {ECO:0000269|PubMed:17380187}.
CC -!- DEVELOPMENTAL STAGE: Expressed during development; especially between 8
CC and 14 hours of development. {ECO:0000269|PubMed:16086850}.
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DR EMBL; AY160092; AAN46872.1; -; Genomic_DNA.
DR EMBL; AAFI02000047; EAL66050.1; -; Genomic_DNA.
DR RefSeq; XP_640241.1; XM_635149.1.
DR AlphaFoldDB; Q8IS20; -.
DR SMR; Q8IS20; -.
DR STRING; 44689.DDB0215004; -.
DR PaxDb; Q8IS20; -.
DR EnsemblProtists; EAL66050; EAL66050; DDB_G0282381.
DR GeneID; 8623780; -.
DR KEGG; ddi:DDB_G0282381; -.
DR dictyBase; DDB_G0282381; gefC.
DR eggNOG; KOG1426; Eukaryota.
DR eggNOG; KOG3417; Eukaryota.
DR HOGENOM; CLU_250987_0_0_1; -.
DR InParanoid; Q8IS20; -.
DR Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR Reactome; R-DDI-171007; p38MAPK events.
DR Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR Reactome; R-DDI-354192; Integrin signaling.
DR Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR Reactome; R-DDI-392517; Rap1 signalling.
DR Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR PRO; PR:Q8IS20; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR Gene3D; 1.20.900.10; -; 1.
DR Gene3D; 2.130.10.30; -; 3.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR009091; RCC1/BLIP-II.
DR InterPro; IPR000408; Reg_chr_condens.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR Pfam; PF00415; RCC1; 3.
DR Pfam; PF00621; RhoGEF; 1.
DR PRINTS; PR00633; RCCNDNSATION.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SMART; SM00325; RhoGEF; 1.
DR SUPFAM; SSF48065; SSF48065; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR SUPFAM; SSF50985; SSF50985; 2.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS00720; RASGEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
DR PROSITE; PS00626; RCC1_2; 2.
DR PROSITE; PS50012; RCC1_3; 4.
PE 2: Evidence at transcript level;
KW Coiled coil; Guanine-nucleotide releasing factor; Reference proteome;
KW Repeat.
FT CHAIN 1..1457
FT /note="Ras guanine nucleotide exchange factor C"
FT /id="PRO_0000384461"
FT REPEAT 1..55
FT /note="RCC1 1"
FT REPEAT 351..401
FT /note="RCC1 2"
FT REPEAT 432..483
FT /note="RCC1 3"
FT REPEAT 485..549
FT /note="RCC1 4"
FT REPEAT 590..647
FT /note="RCC1 5"
FT DOMAIN 650..971
FT /note="DH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00062"
FT DOMAIN 989..1109
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 1232..1454
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT REGION 75..196
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 209..264
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 282..313
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 376..404
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 703..762
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1127..1210
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1138..1211
FT /evidence="ECO:0000255"
FT COMPBIAS 75..121
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 128..160
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 161..187
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 223..240
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 241..264
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 282..300
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 722..739
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 740..762
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1457 AA; 163235 MW; 23F79A14AD357AB0 CRC64;
MSVFTFGHGS NGALGLGKIT DDTCPTPQKV NYFTEIDKRV KKVACGSYHT VFVTDDEHLY
ICGIKKDPKH GTTLFYTSPP PTTTTTTTTT SSTSATTTTT NGMVNEKNNN NKNNNGEKIV
NSKPESVIIK DGASNTTNDS TSSSSTSTSS LSSSLPPTNI ETPRDKKKPP IKLDKGIPHH
RSTRELIQRP ESPLLNKLLT SKAEMEFKHY ESNEKSKDEM KDNENENEED EDDDDDDSTI
RQNEDKESSL IDDNESETSL RINKKDAENI TNNIRLIKEL DEQQQQPQQP QQNLKGIMNQ
PPLPTNDDLD EDPNIHFTPI RISTHFLENS PLNTIKQVAL GNYHIVLLTE GGNVWTWGSN
SNGQLGYLVD TQQQQQQQLQ QQQSMSSLQP SASSSSPSSS SLQTTCTPKM VEIKCVKYIA
TGVKNTAALT EWGELYMWGI NEHGELGLGD TIDRRTPTRV IRLKNEVVST IACSSTHSAC
YTESGKMYVW GQSDETGKIQ AIPSPLPIHS YLDREIKSPN GGGVGGFFGG GGKIKQLACG
QRCIAVLTQM GEVFMWEIPG IPIPLRNALE NHSVRNIVMG NFHLVCLTDS GEVVTIGRNK
TGQLGRTDAE NEPGIVKQLS FDPDGLNSDE FVVSIAAGEF HSVALVENTP KTKLALQLVR
MQRNYLRQLN ILNNIYYKSM MSMASPYDPV VLSMMNNGSA STLPPSLKGL SGGLPDNANN
TIKNGKDKDN HHNGDSNGHH SNGHYHGNGN NGNNSITTSN SISSPSLLGS GGTLKSRSAS
IATIRGLFGI NHMLHHSQSQ SFQEENSTVT EDEIKEIFSD IEALSKLTEK FLSYLDQRMD
NWDPVSKVLG DIFLDEALMA AYRIYIPYSD SYNTSCMTLF NIRRRNERLS NIIKDCEKKS
KSFGVKLDQE FVKEIDLKSL LLSPLQNIPR IYILLRELGS DDSGNINPRD IDLINFAATK
FQVLLERMNQ NFQFVNAVEI LHCSSNEYGN PQIMGGSLPQ LVDKLTHHNI SDPNFRDVFL
LTFRAFTTPC NLFDLLVETF QRQKHLKNGR VVNVITSWVV HHFYDFEKDK LSDFTQDPNH
NPQLLSEKLE QFIYSEDHQS VSQIKLQYFS HRKRLESSIN LIDNQKLTQN EITTPPPLQI
QNNNQNNNLE NNNNNNNNNN TTTTTTTPND NINDINNNNN NNNNNNNNNN NNNNNNNNNN
NNNNNNNNLT NSTIKLVVQP PIPINFNLLD SQPIEVAQTL TIMDHHYFAM IDKREFLGQR
WAKNKSPNIQ ISTDHFNRTS QVVVTEILKS KNSKQRSATL GYFISVAYCC FELNNLSGTA
SIIYGLNSAS IQRLKKSWSK LPKESMIAFE YLDKIVTPMK NYISLRHLMT TIQPPCVPFL
GTYLKDLTFI EEGNPSIIGG LINFYKQRKI AEVIFQIQQH QQVVYSAIRS NPTIKAFLMS
SHTFDDKQAQ KISSEAE
