GEFD_DICDI
ID GEFD_DICDI Reviewed; 668 AA.
AC Q8IS19; Q54H48;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Ras guanine nucleotide exchange factor D;
DE AltName: Full=RhoGAP domain-containing protein D;
GN Name=gefD; Synonyms=RasGEFD; ORFNames=DDB_G0289667;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AX4;
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Expressed during development; especially between 8
CC and 12 hours of development. {ECO:0000269|PubMed:16086850}.
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DR EMBL; AY160093; AAN46873.1; -; Genomic_DNA.
DR EMBL; AAFI02000148; EAL62545.1; -; Genomic_DNA.
DR RefSeq; XP_636070.1; XM_630978.1.
DR AlphaFoldDB; Q8IS19; -.
DR SMR; Q8IS19; -.
DR STRING; 44689.DDB0201637; -.
DR PaxDb; Q8IS19; -.
DR EnsemblProtists; EAL62545; EAL62545; DDB_G0289667.
DR GeneID; 8627280; -.
DR KEGG; ddi:DDB_G0289667; -.
DR dictyBase; DDB_G0289667; gefD.
DR eggNOG; KOG3417; Eukaryota.
DR HOGENOM; CLU_411313_0_0_1; -.
DR InParanoid; Q8IS19; -.
DR OMA; INTEKFR; -.
DR PhylomeDB; Q8IS19; -.
DR Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR Reactome; R-DDI-171007; p38MAPK events.
DR Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR Reactome; R-DDI-354192; Integrin signaling.
DR Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR Reactome; R-DDI-392517; Rap1 signalling.
DR Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR PRO; PR:Q8IS19; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005096; F:GTPase activator activity; IEA:UniProtKB-KW.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR GO; GO:1903013; P:response to differentiation-inducing factor 1; HDA:dictyBase.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.555.10; -; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR InterPro; IPR000198; RhoGAP_dom.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR Pfam; PF00620; RhoGAP; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SMART; SM00324; RhoGAP; 1.
DR SUPFAM; SSF48350; SSF48350; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
DR PROSITE; PS50238; RHOGAP; 1.
PE 2: Evidence at transcript level;
KW GTPase activation; Guanine-nucleotide releasing factor; Reference proteome.
FT CHAIN 1..668
FT /note="Ras guanine nucleotide exchange factor D"
FT /id="PRO_0000384462"
FT DOMAIN 27..224
FT /note="Rho-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00172"
FT DOMAIN 237..362
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 433..663
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
SQ SEQUENCE 668 AA; 77224 MW; 5858EFBC6C38D28B CRC64;
MGNSKQIKRS LTGSILLKKQ ISQIFFKKLE SIFGIALAED TSDRLMDYLN KSIDYLLDHS
VIEGLFSIEI EESKLKSTIE SIESTLITDF SLTTDPCYVA STFILYFSKL PNLLLSKISS
QLIHSVEISH EEYRINVIRS LLYSLPLQNR NILKMVLHFL KKFSLACNNN NNNNSENNNT
IVEDIEVQSA YNRFTKAFMS TTPGNFEISC KAIRLMVMDI DEFDKLPQDI QYMVKDGESI
VKAATFDRLV EKLFDLSYGF KDPDYNYTVF HTYDYYTTSV ELLGKIVHYY RIACTLTTKL
QMEVSITILS VAMFWMKIHH NHLMTDLQFL QKLKIFLDSV PQVPPTQVTY FTYFQTFFKP
VIEPLKPLYE RGNSFLGPNL TQSSGTSKKK NQLIEKMMIN NNINNNNNIN NNVNSNSNNN
FNNNIDIDIY NIGSNIIAQQ ITIIDNEMLM AIPPSQFLHK SFSKESKSPQ FHDMVSKFNE
WARWTSSEIL SKEKLVERVV TLSFFIDLAK SCVELGNYNA ANAIVGGLNH SSISRLKLTW
ERLSTKVTQD YDRLLSLFDL SMNYKNYRDE IKSTKAKIIP YLGLFPKDLI AIEEGNDNFT
NNNLINTEKF RLLYQTIKKI QSYQQPLFTF KTSEPIKQYL KNISNGLSEE KDFHSISHKL
EPRQQQTQ
