GEFE_DICDI
ID GEFE_DICDI Reviewed; 1037 AA.
AC Q8IS18; Q55CK1;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Ras guanine nucleotide exchange factor E;
DE AltName: Full=RasGEF domain-containing protein E;
GN Name=gefE; Synonyms=rasGEFE; ORFNames=DDB_G0269252;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=AX4;
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION.
RX PubMed=16234315; DOI=10.1093/bioinformatics/bti726;
RA Booth E.O., Van Driessche N., Zhuchenko O., Kuspa A., Shaulsky G.;
RT "Microarray phenotyping in Dictyostelium reveals a regulon of chemotaxis
RT genes.";
RL Bioinformatics 21:4371-4377(2005).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP (By
CC similarity). Seems to play a role in chemotaxis. {ECO:0000250,
CC ECO:0000269|PubMed:16234315}.
CC -!- DEVELOPMENTAL STAGE: Expressed during development; especially from 12
CC hours of development. {ECO:0000269|PubMed:16086850}.
CC -!- DISRUPTION PHENOTYPE: Poor in phototaxis assays.
CC {ECO:0000269|PubMed:16086850}.
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DR EMBL; AY160094; AAN46874.1; -; Genomic_DNA.
DR EMBL; AAFI02000005; EAL71976.1; -; Genomic_DNA.
DR RefSeq; XP_646480.1; XM_641388.1.
DR AlphaFoldDB; Q8IS18; -.
DR SMR; Q8IS18; -.
DR STRING; 44689.DDB0191289; -.
DR PaxDb; Q8IS18; -.
DR PRIDE; Q8IS18; -.
DR EnsemblProtists; EAL71976; EAL71976; DDB_G0269252.
DR GeneID; 8617442; -.
DR KEGG; ddi:DDB_G0269252; -.
DR dictyBase; DDB_G0269252; gefE.
DR eggNOG; KOG3417; Eukaryota.
DR HOGENOM; CLU_293285_0_0_1; -.
DR InParanoid; Q8IS18; -.
DR OMA; CNNRIEY; -.
DR PhylomeDB; Q8IS18; -.
DR Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR Reactome; R-DDI-171007; p38MAPK events.
DR Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR Reactome; R-DDI-354192; Integrin signaling.
DR Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR Reactome; R-DDI-392517; Rap1 signalling.
DR Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR Reactome; R-DDI-9013423; RAC3 GTPase cycle.
DR PRO; PR:Q8IS18; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IMP:dictyBase.
DR GO; GO:0090630; P:activation of GTPase activity; IMP:dictyBase.
DR GO; GO:0046956; P:positive phototaxis; IMP:dictyBase.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR GO; GO:1903013; P:response to differentiation-inducing factor 1; IMP:dictyBase.
DR GO; GO:0043052; P:thermotaxis; IMP:dictyBase.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; PTHR23113; 2.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Guanine-nucleotide releasing factor; Reference proteome.
FT CHAIN 1..1037
FT /note="Ras guanine nucleotide exchange factor E"
FT /id="PRO_0000384463"
FT DOMAIN 496..694
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 726..1003
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT REGION 65..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 114..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 166..387
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 414..437
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 451..472
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 602..628
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 907..935
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1004..1037
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 5..35
FT /evidence="ECO:0000255"
FT COMPBIAS 166..360
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 373..387
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1009..1037
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1037 AA; 114738 MW; 895499833FBFF758 CRC64;
MDHTECNNRI EYLQNKVLEL ESLNENLKGQ LEYFQTKFLE SNLNSEGKDI SSCLTEIYNS
LSLDNNNNNS NSNSNSNNNS SNSNNNSYSN FNSNNNTTNN TKVIKNRNFK INLTNSNSTN
SSPYIFGNNK DNSNNSNNSN NNNSNTELSN DHLSLEDNIA TTNTTTTTTI NTSNSNNSNN
NNNNNNNNNN NNNNNNNNNN KPTSNRPPVA PRSFIRSNSD TFITPPGSPT SSRNSPTNKS
SPQFLSPLSK SPLSQSTQST TVSSPSPSWT TTVPQSKMRS ETIVQSKSPY SPDTNISNKL
INELASNIGA PNSNGSESPS KNSPRSLNSN NNNSSATTSI TTPPTTSTPT PTTSTTTTTT
TERRPEDRRS KTSPFPNVGT TTPPLSNSAH LVRTQSASGA NAVKPQSQLS TSCTVNMIGG
GGTPRSNNSS NGSLYHSMSS NGLIENLSTS SSLSLSSTSP SPNNSQQNLT NPFEIGADDE
LEVLDDPRLV MVKTENGFIV KGGTIEKLVN RLIAKHDPDF TSAFLLTHKS FTTSIELLDL
LIEFYVNNKD SSSINSNSSS SSSSAVASSS FSNINCNISD LSSSTSSTNS LLINSITNSP
IINSNNQNNQ NNNNNNNNNN NNNNNQLEIN GDKKKKAIRL KITNVIKSWV DKHYYDFSED
KQLTLKLDQF ITNHIMFDMD KIGFNLKRLL TNDRVVPVPT FTQGPPTPIP PKMKSNGSEI
NFKDLDPTEI ARQLTLYESD LFRKIGAKEC LGQAWNKDGK EENAPNIVSF IKRFNQVSSW
VATEIVRQEK LKDRVSYIKR FILVAQECRK LNNFNATMEI LSGLQNSSVY RLRKTWERVE
SKPLLKNTLD ELMSLMSSGA NYKNYIQELH NIHPPCIPYL GVYLTHLTFI EDGMKNVLTT
TTATTTNTTT TTTTTTTTTT TNTTTSNNNN QQQLNGANRS FDDVIINFEK CRKISVVIRE
IKQYQQQQYH LHTEEFTLRY LSNLPSIQTQ KSMYKLSLIC EPKEKETSSF DSGYGSVSDR
PSKKEFSVTS LLNSFKS
