GEFF_DICDI
ID GEFF_DICDI Reviewed; 1127 AA.
AC Q54C94; Q8IS17;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 102.
DE RecName: Full=Ras guanine nucleotide exchange factor F;
DE AltName: Full=RasGEF domain-containing protein F;
GN Name=gefF; Synonyms=rasGEFF; ORFNames=DDB_G0293006;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AX4;
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=18590548; DOI=10.1186/1471-2180-8-109;
RA Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R.,
RA Ivens A., Martinez J.L., Escalante R.;
RT "Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common
RT and specific effects from PAO1 and PA14 strains.";
RL BMC Microbiol. 8:109-109(2008).
RN [4]
RP IDENTIFICATION.
RX PubMed=18559084; DOI=10.1186/1471-2164-9-291;
RA Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B.,
RA Skelton J., Ivens A., Bozzaro S.;
RT "Genome-wide transcriptional changes induced by phagocytosis or growth on
RT bacteria in Dictyostelium.";
RL BMC Genomics 9:291-291(2008).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Expressed during development; with a peak of
CC expression at 4 hours of development. {ECO:0000269|PubMed:16086850}.
CC -!- INDUCTION: Down-regulated by Pseudomonas aeruginosa, PAO1 strain and
CC PA14 strain infection and by phagocytic stimuli.
CC {ECO:0000269|PubMed:18590548}.
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DR EMBL; AY160095; AAN46875.1; -; Genomic_DNA.
DR EMBL; AAFI02000199; EAL60855.1; -; Genomic_DNA.
DR RefSeq; XP_629325.1; XM_629323.1.
DR AlphaFoldDB; Q54C94; -.
DR SMR; Q54C94; -.
DR STRING; 44689.DDB0215365; -.
DR PaxDb; Q54C94; -.
DR PRIDE; Q54C94; -.
DR EnsemblProtists; EAL60855; EAL60855; DDB_G0293006.
DR GeneID; 8629049; -.
DR KEGG; ddi:DDB_G0293006; -.
DR dictyBase; DDB_G0293006; gefF.
DR eggNOG; KOG0379; Eukaryota.
DR eggNOG; KOG3417; Eukaryota.
DR HOGENOM; CLU_279577_0_0_1; -.
DR InParanoid; Q54C94; -.
DR OMA; DKCPWDF; -.
DR Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR Reactome; R-DDI-171007; p38MAPK events.
DR Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR Reactome; R-DDI-354192; Integrin signaling.
DR Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR Reactome; R-DDI-392517; Rap1 signalling.
DR Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR PRO; PR:Q54C94; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0044354; C:macropinosome; IDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0044351; P:macropinocytosis; IMP:dictyBase.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR Gene3D; 2.120.10.80; -; 2.
DR InterPro; IPR015915; Kelch-typ_b-propeller.
DR InterPro; IPR006652; Kelch_1.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00612; Kelch; 3.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF117281; SSF117281; 2.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS50896; LISH; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 2: Evidence at transcript level;
KW Guanine-nucleotide releasing factor; Kelch repeat; Reference proteome;
KW Repeat.
FT CHAIN 1..1127
FT /note="Ras guanine nucleotide exchange factor F"
FT /id="PRO_0000384464"
FT REPEAT 212..261
FT /note="Kelch 1"
FT REPEAT 262..311
FT /note="Kelch 2"
FT REPEAT 313..366
FT /note="Kelch 3"
FT REPEAT 367..418
FT /note="Kelch 4"
FT REPEAT 420..469
FT /note="Kelch 5"
FT DOMAIN 557..589
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT DOMAIN 673..804
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 835..1062
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT REGION 1..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 96..154
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1090..1127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 27..49
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 61..82
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 579
FT /note="A -> E (in Ref. 1; AAN46875)"
FT /evidence="ECO:0000305"
FT CONFLICT 646
FT /note="F -> L (in Ref. 1; AAN46875)"
FT /evidence="ECO:0000305"
FT CONFLICT 653
FT /note="N -> T (in Ref. 1; AAN46875)"
FT /evidence="ECO:0000305"
FT CONFLICT 661
FT /note="N -> T (in Ref. 1; AAN46875)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1127 AA; 126465 MW; FDA91DFC452400B4 CRC64;
MTDKLKFKTS LLNKVPPPPP KSNQPSPSTS TPASPNVNST NNSPSVSPAT TSPIPSPGMS
PLLTPENNNN NTNIPHQTSL LNNSISNKSS VSFLNNSGGN TINKSSPLSQ SSSNIKNGGP
IRTSTTLAQF SGSSLPNTEN SSPPPSSSLI SSSSSPTAES LLYSEDSIAS GGTVTVDPNT
KNGNIYNGNG LRNNSADILP HNNHTYCVGD DGFYLFGGTL PDGSYTNDFY TFQFAIKAWT
ILTFGSAPSI RTRHTGVLYN NSMYIFGGYS PSGPKNDIYV FSFDTQTWSE VQTEGTKPSP
RYGHTAVVES GHMIVFGGIS CDQTTKQQTV NNDIFSLNLD TKQWSQVLST CPPSPRTHHT
ATMHKGNMYV FGGQDQQSNQ VEDIVHCYTW ASNSWKSIQF EGSSMTPRSD HSAVLFQDSI
FISGGSSKSQ TSQNLEIYEY DLYQKKCFKI SSSTIVQNRI SHSSVVKGNS ILFWGGCTDN
SFDYFSFGKD EFEEDYQDDY ESNRVQNIPK ELWEASLMKK HPEILELREK TLAFTGSKSF
AKTLATPSFS ENRLALSHQF VLQLIMEYLE RNTYHKVIAA IQKESGVLHQ PTESGESRLV
SLLRLVKPRL RNKNVFDTDL SLFSKEEGND PEVAVVDHLY HDYRHFDEEE DINVWEEGED
NNRNIRKVET DNNKVQIKAA TFNKLIHYLA PKEKAFDPNF LKVFLYTHSS FTTSEKLLKK
LIQRYQVPNS NANEPKYKSE VVEPVRQRVV DVLKYWVDKC PWDFNAGPTS SVLVATLNNF
IDGSLTRDGN SNIKKLRELK KKLQHEDVRP YSEPPPEPKV PKNIFSPQLT LAHIDELEIA
RQMTLVESKL FGAIPPPEFM VRVIGYGEFQ YNMATSPNLM TFVNRATDVS RWIVHTVLNE
SRDKKNKMKM LDKFIKTTEC LRQLNNFQTL HSVLQGLQHP LLLSRPDLFT PRHREIIADH
EMLFSKIDNY KLYREALARS QPACVPWIDI IREDFAQIER DQPSNMNNLI NFTKRQNLYE
ILSKIGHYQF PYNLQIVHQV ATFVNKLPKY SEYDLNLLSE SLQSQVNSPL AGVYQPSLIG
TTGSSSSINL GSARELNNSN RDSNNITGSS SNNNSNSSNS LSPIVKL
