ID   GEFG_DICDI              Reviewed;        1556 AA.
AC   Q8ST25; Q55AB0;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 113.
DE   RecName: Full=Ras guanine nucleotide exchange factor G;
DE   AltName: Full=RasGEF domain-containing protein G;
GN   Name=gefG; Synonyms=rasGEFG; ORFNames=DDB_G0272032;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC   STRAIN=AX4;
RX   PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA   Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA   Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT   "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT   roles.";
RL   Genome Biol. 6:R68.1-R68.12(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Faintly expressed between 12 and 14 hours of
CC       development. {ECO:0000269|PubMed:16086850}.
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DR   EMBL; AY160096; AAN46876.1; -; Genomic_DNA.
DR   EMBL; AAFI02000007; EAL71450.1; -; Genomic_DNA.
DR   RefSeq; XP_645356.1; XM_640264.1.
DR   AlphaFoldDB; Q8ST25; -.
DR   SMR; Q8ST25; -.
DR   STRING; 44689.DDB0185195; -.
DR   PaxDb; Q8ST25; -.
DR   EnsemblProtists; EAL71450; EAL71450; DDB_G0272032.
DR   GeneID; 8618245; -.
DR   KEGG; ddi:DDB_G0272032; -.
DR   dictyBase; DDB_G0272032; gefG.
DR   eggNOG; KOG3417; Eukaryota.
DR   HOGENOM; CLU_246163_0_0_1; -.
DR   InParanoid; Q8ST25; -.
DR   OMA; HLAPNIR; -.
DR   Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR   Reactome; R-DDI-171007; p38MAPK events.
DR   Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR   Reactome; R-DDI-354192; Integrin signaling.
DR   Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR   Reactome; R-DDI-392517; Rap1 signalling.
DR   Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR   Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR   Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR   PRO; PR:Q8ST25; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR   GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR   CDD; cd00155; RasGEF; 1.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   InterPro; IPR036047; F-box-like_dom_sf.
DR   InterPro; IPR001810; F-box_dom.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   PANTHER; PTHR23113; PTHR23113; 2.
DR   Pfam; PF12937; F-box-like; 2.
DR   Pfam; PF00617; RasGEF; 1.
DR   Pfam; PF00618; RasGEF_N; 1.
DR   SMART; SM00256; FBOX; 2.
DR   SMART; SM00147; RasGEF; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   SUPFAM; SSF81383; SSF81383; 2.
DR   PROSITE; PS50181; FBOX; 1.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
PE   2: Evidence at transcript level;
KW   Guanine-nucleotide releasing factor; Reference proteome; Repeat.
FT   CHAIN           1..1556
FT                   /note="Ras guanine nucleotide exchange factor G"
FT                   /id="PRO_0000384465"
FT   DOMAIN          307..353
FT                   /note="F-box 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT   DOMAIN          679..726
FT                   /note="F-box 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT   DOMAIN          1116..1244
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT   DOMAIN          1317..1548
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT   REGION          19..63
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          93..141
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          156..239
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          254..296
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          385..416
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          762..1101
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1254..1279
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        19..62
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        190..221
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        762..933
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        973..1101
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1556 AA;  170504 MW;  188F80979F1310E8 CRC64;
     MDKINNENDK INISIKAPIG NIEDNNNNSN NNSNNNSVSN SNNNSSISIN SGSNTPTNSK
     NRGIKIFDLD DPFTSKRLSG FFSPESLAEL SKLQLNDTDN NDNLTTTTTT TTTTTTTTTT
     TNTITSKNNS PNTSPLLSST TISSITSGAP IKKRFERIPS GLSHVTNHYD DDDDDESSSS
     EEDFDSTELV CRPNKTSSTT ATTTTTTTSV SPLTSSFVMR RPSSPSPPPS CSSPTLSPSL
     SATRRLSGNL VKVATDSDNQ SLEPCNNKTI VDNNDNDNDN NNNHNNNNNN NNNNNSTEDI
     SIKPICNKTF LDLDEKIYLK IFGYLFAEDL CSINRVSKHL CNIINNQQLW KDLFSIYIKI
     YSEPSDIYIW DEDLYSIYNN NNNNNNNNNN NNNNNNNNNN SNISNNNNNI NNSNGNNNPY
     FTGGVSNSSI GAIGGSIGSS AGSNIALKLR SNTSIPIIER GRSNTIAMPT PTFINEINML
     NSNNSVNNNN NISNNNTINS NNAGVIINPS GTVNNNINNI NSSNSINSSS SSGSNINLSN
     NNSSTIHGSY ISSNLILNSV LRKFTPTTSV PSTNEVTDFI ESCHHFPSKL LICKLVQRYL
     VPREFQRHLS DIDWVKMVER PIQLRVGKLL KKVIDQKKVN FDYGTMLILR SFIRGYIQST
     SDMAKGLIVG LQKKQVLKVF DISRVSDILL IKIFRNLDSI KDLSVCQRVS KRWNKAIAIS
     SLWEQLYLNY RKKLHHEGDI NIWDDPSLHT VEYLYEFFDQ IQPSPQQQQQ QQQQQQQQQQ
     QQQQPQLQTN ESNNNNEDIK QNDNNNNNSN NNGLNNLQKL IEENLEGSNL SNGGNSGSSF
     SPFNPLSGSN GGSSFGPFGS GGGGGSNSNI GGSSNQLNSS SGGGGSGFLA MASGGSSVSS
     VTSTPSTIST NCTPTGGSTT NSPNFQSPMV SSSTVLPPPI PFSPNLSSPR SMSISLSSPP
     PLPNKPPELK LPDSSLSPNS SFNNNLSSTS ISIPSTPTTT PSSSLLTFIP PNTTSTTTTT
     STTNITSTTM PISAATTTTT TTTSTTSTII QPIQPIPPVN NNGSQSDLSK ISDLNANPNT
     TTTTTTNTIE SSSSSSSITN NNENIIEPIK SPQPSMINVQ KLMNLNQLVD KLTSITVATE
     ISEVNACLAT YRTFISTSQL VEKLFQRYHI PRASNIKSIL DWKQRIETPI QVKVCKVFKK
     LIDDHFDDFN GTIIELFKVF LLHIIDKQSP LTNHLIRSFS RKLSGENGST GSVIGITGGK
     SSRKGGGSGS GSGSGGGSGS SIIGGAVGGT IKASQSKIGR MMSVRKAQQF NDIISLPAEE
     IAKQLTLIEF EIFGKIQSSE FLNQSWAKEK TFHLAPNIRA SIDRFNTVTK WVCTIILKEE
     KIRTRTKIMS KLLKVAKNLR SYSNFHTLMA ILSGLNEIHI YRLKFTQQEL KPKIQKISSE
     LQTLMSVEGN HEAYRTELSN VDPKQSCIPY LGVYLKDLTF IQDDTNKKGD GINIKQSLNL
     YNILKTIQNF QKNPYSFEEF PKIKETLLNL PVWTEDNLYR VSMQREPRNC KRSDLI