GEFI_DICDI
ID GEFI_DICDI Reviewed; 824 AA.
AC Q8IS15; Q54YQ2;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=Ras guanine nucleotide exchange factor I;
DE AltName: Full=RasGEF domain-containing protein I;
GN Name=gefI; Synonyms=rasGEFI; ORFNames=DDB_G0277915;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AX4;
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=18590548; DOI=10.1186/1471-2180-8-109;
RA Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R.,
RA Ivens A., Martinez J.L., Escalante R.;
RT "Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common
RT and specific effects from PAO1 and PA14 strains.";
RL BMC Microbiol. 8:109-109(2008).
RN [4]
RP IDENTIFICATION.
RX PubMed=18559084; DOI=10.1186/1471-2164-9-291;
RA Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B.,
RA Skelton J., Ivens A., Bozzaro S.;
RT "Genome-wide transcriptional changes induced by phagocytosis or growth on
RT bacteria in Dictyostelium.";
RL BMC Genomics 9:291-291(2008).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Expressed during development; with a peak of
CC expression at 12 hours. {ECO:0000269|PubMed:16086850}.
CC -!- INDUCTION: Up-regulated by Pseudomonas aeruginosa, PAO1 strain and PA14
CC strain infection and down-regulated by phagocytic stimuli.
CC {ECO:0000269|PubMed:18590548}.
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DR EMBL; AY160098; AAN46878.1; -; Genomic_DNA.
DR EMBL; AAFI02000023; EAL68130.1; -; Genomic_DNA.
DR RefSeq; XP_642150.1; XM_637058.1.
DR AlphaFoldDB; Q8IS15; -.
DR SMR; Q8IS15; -.
DR STRING; 44689.DDB0215006; -.
DR PaxDb; Q8IS15; -.
DR PRIDE; Q8IS15; -.
DR EnsemblProtists; EAL68130; EAL68130; DDB_G0277915.
DR GeneID; 8621358; -.
DR KEGG; ddi:DDB_G0277915; -.
DR dictyBase; DDB_G0277915; gefI.
DR eggNOG; KOG3417; Eukaryota.
DR HOGENOM; CLU_343697_0_0_1; -.
DR InParanoid; Q8IS15; -.
DR OMA; PKNIWSH; -.
DR PhylomeDB; Q8IS15; -.
DR Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR Reactome; R-DDI-171007; p38MAPK events.
DR Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR Reactome; R-DDI-354192; Integrin signaling.
DR Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR Reactome; R-DDI-392517; Rap1 signalling.
DR Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR PRO; PR:Q8IS15; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS50896; LISH; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 2: Evidence at transcript level;
KW Guanine-nucleotide releasing factor; Reference proteome.
FT CHAIN 1..824
FT /note="Ras guanine nucleotide exchange factor I"
FT /id="PRO_0000384467"
FT DOMAIN 223..255
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT DOMAIN 426..551
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 585..816
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT REGION 1..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 65..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 330..354
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 398..425
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..167
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 824 AA; 91994 MW; 1D1D83D797BC10A6 CRC64;
MSNPVCISNS TNGSSNSLNG ESVSPNRLGS SPGSPISKAS SFDLNGKKPT KSNVVRLLLN
RTNSGSNLLS KRRTSETGDD DSNSSVGLLN NSTGSIGKMN TPESSPKSSY ILSSSIGSGG
SGGGGGSSGS LQNLDSASNN SSGPRSRSGS LGKNNSSQQN NNNLILDPNF NNIDKSLWTV
RSLKEHSNLV DIMERIKPAS RAISFGQIYA SEETQFEFDP VIGRDNILQL ILQHLQFEGL
MDSRKILEEE AKIQYPEYTF NESRLVTLLR AVIKDSDKVF DLTLNDRDKD SQQKLEEHLA
FLGLFKDESQ TNMVEDVNIY DEPENSNIIY VDEKDNDKPS KDSPTTATTT TTATTIAPST
SINNLSSLSV SISSNNINNN NNNNNNINNL NSTQLISNTQ QQQATPNTPP QGLKSTQSIT
GSTGTLGPQV KAASLNKLVI LLTPENNHDL EYTKTFLLMY QSFTTPEILL QKLIQRYHVP
QKAGQSVAEW RQRSTHIQLR VLNVLKTWIK DYFSDFSEKL ILAIKSLLES MRQTGNMSYA
KVISDALNSG LKKSGRNNTV FTVSAPEPKV PKNIWSHNLD IFSVDEEEIS RQLTLMDFEI
FSNIKSTELL NQCWNKPKLR HRSPNVLELI GRFNEISQWT ATSILSWPKV KDRARIMGKF
IKIAEYCMKH LNNFNTSMAI LSGLNASSVH RLKFTKEELP RHTQQVYTEL QFHLSSAQAY
KEYRALLAKA NPPCLPYLGV YLTDLTFFEE GNPDFIQGFI NFGKRKLIYG SISNVQSFQN
TKYNLQPVYQ IAKLLKGFKL LEENELYTRS MSFEPRNKER SEIL
