ID   GEFJ_DICDI              Reviewed;         812 AA.
AC   Q8IS14; Q54YV8;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Ras guanine nucleotide exchange factor J;
DE   AltName: Full=RasGEF domain-containing protein J;
GN   Name=gefJ; Synonyms=rasGEFJ; ORFNames=DDB_0215008;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC   STRAIN=AX4;
RX   PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA   Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA   Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT   "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT   roles.";
RL   Genome Biol. 6:R68.1-R68.12(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during development.
CC       {ECO:0000269|PubMed:16086850}.
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DR   EMBL; AY160099; AAN46879.1; -; Genomic_DNA.
DR   EMBL; AAFI02000023; EAL68129.1; -; Genomic_DNA.
DR   RefSeq; XP_642094.1; XM_637002.1.
DR   AlphaFoldDB; Q8IS14; -.
DR   SMR; Q8IS14; -.
DR   STRING; 44689.DDB0215008; -.
DR   PaxDb; Q8IS14; -.
DR   EnsemblProtists; EAL68129; EAL68129; DDB_G0277913.
DR   GeneID; 8621305; -.
DR   KEGG; ddi:DDB_G0277913; -.
DR   dictyBase; DDB_G0277913; gefJ.
DR   eggNOG; KOG3417; Eukaryota.
DR   HOGENOM; CLU_343697_0_0_1; -.
DR   InParanoid; Q8IS14; -.
DR   OMA; HYSSHTK; -.
DR   PhylomeDB; Q8IS14; -.
DR   Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR   Reactome; R-DDI-171007; p38MAPK events.
DR   Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR   Reactome; R-DDI-354192; Integrin signaling.
DR   Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR   Reactome; R-DDI-392517; Rap1 signalling.
DR   Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR   Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR   Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR   PRO; PR:Q8IS14; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR   GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR   CDD; cd00155; RasGEF; 1.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   InterPro; IPR006594; LisH.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   PANTHER; PTHR23113; PTHR23113; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   Pfam; PF00618; RasGEF_N; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SMART; SM00229; RasGEFN; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   PROSITE; PS50896; LISH; 1.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
PE   2: Evidence at transcript level;
KW   Guanine-nucleotide releasing factor; Reference proteome.
FT   CHAIN           1..812
FT                   /note="Ras guanine nucleotide exchange factor J"
FT                   /id="PRO_0000384468"
FT   DOMAIN          214..246
FT                   /note="LisH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT   DOMAIN          409..535
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT   DOMAIN          573..804
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT   REGION          1..147
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          320..382
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..108
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        120..147
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        320..344
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        345..382
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   812 AA;  91804 MW;  5CAABA4E6FBB7EDE CRC64;
     MSNPVSINNS GSISNSNLNN ESLSPSRLSS SPNSKSMELN GKRTLKSSVV KTLLNRSSSG
     NNLNNTVTTR RAAGSFTSNY QNIYTPNNNS YNSSNNNNNN NNDDDTVSVD GDNPPDSPKS
     TKDHSNSVGS NSGGGGGGSS GGLNMSMNNI KQQQQDNLIK QQQKDRSLWT VRSLKEHAEV
     LDIMERLKPV NRAISFGQIY AREETQFDFD PLIGRDTMLQ LILQHLQFEG LMDSRKLLEE
     EARVQYPEYA INESRLVTLL RTALKDSERV FQLTLDDKKR DSQQQLEEHL AFLGLFKDET
     ATDIMGEDVN IYDEPENSNI IYVDDKEKEK EKEKEKEKEK DKFGPNSTNS LSGSGSSPNI
     PSGMNNNSSS IGNNSIGNSN SYSNGELSPL SASTLLMMSP QNKNSIETNK PQVKAASLNK
     LVILLTPDNN HDLEYTKTFL LTYQSFTTPE MLLEKLVQRY HVPQKPGQSL DDWKKSAVPI
     QLRVANVIRT WIKDYFSDFN DKLIQNVKSL YENMKQSGNM SHAKILSESL NAKIKGMVGM
     DDQKRAPTFT SPAPEPKVPK NIWSQTLDIF SVDEEEIARQ LTLMDFEIFS AIKSTELLNQ
     SWNKPKLRHR SPNVLTLINR FNEISQWTAT SILSYPKVKD RARIMAKFIK IAEYCMRHLN
     NFNTSMAILS GLNASSVHRL KFTKEELPKH TQQVYTELQF HLSSAQAYKE YRALLAKANP
     PCLPYLGVYL TDLTFFEEGN PDFIQGFINF GKRKLIYGSI SNVQSFQNAK YNLQPVYQIT
     KLLKGFKLLE ENDLYSRSMS FEPRNKERSE IL