GEFK_DICDI
ID GEFK_DICDI Reviewed; 1557 AA.
AC Q54FF3; Q8IS13; Q8T6G6;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Ras guanine nucleotide exchange factor K;
DE AltName: Full=RasGEF domain-containing protein K;
GN Name=gefK; Synonyms=rasGEFK; ORFNames=DDB_G0290901;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AX4;
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 167-1557.
RC STRAIN=AX2;
RA Mueller R., Eichinger L., Noegel A.A.;
RT "RasgefK, a new member of the Dictyostelium discoideum Rasgef family.";
RL Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Expressed during development; especially from 4
CC hours of development and with a peak of expression at 12 hours of
CC development. {ECO:0000269|PubMed:16086850}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL83292.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AY160100; AAN46880.1; -; Genomic_DNA.
DR EMBL; AAFI02000172; EAL61966.1; -; Genomic_DNA.
DR EMBL; AF474377; AAL83292.1; ALT_FRAME; mRNA.
DR RefSeq; XP_635469.1; XM_630377.1.
DR AlphaFoldDB; Q54FF3; -.
DR SMR; Q54FF3; -.
DR STRING; 44689.DDB0191358; -.
DR PaxDb; Q54FF3; -.
DR PRIDE; Q54FF3; -.
DR EnsemblProtists; EAL61966; EAL61966; DDB_G0290901.
DR GeneID; 8627885; -.
DR KEGG; ddi:DDB_G0290901; -.
DR dictyBase; DDB_G0290901; gefK.
DR eggNOG; KOG3417; Eukaryota.
DR HOGENOM; CLU_246117_0_0_1; -.
DR InParanoid; Q54FF3; -.
DR OMA; EDFCHES; -.
DR Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR Reactome; R-DDI-171007; p38MAPK events.
DR Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR Reactome; R-DDI-354192; Integrin signaling.
DR Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR Reactome; R-DDI-392517; Rap1 signalling.
DR Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR PRO; PR:Q54FF3; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS00720; RASGEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Guanine-nucleotide releasing factor; Reference proteome.
FT CHAIN 1..1557
FT /note="Ras guanine nucleotide exchange factor K"
FT /id="PRO_0000384469"
FT DOMAIN 1058..1177
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 1316..1549
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT REGION 1..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 146..181
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 195..238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 283..347
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 558..619
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 881..937
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1254..1303
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 310..338
FT /evidence="ECO:0000255"
FT COILED 591..629
FT /evidence="ECO:0000255"
FT COMPBIAS 1..15
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..121
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 283..312
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 313..347
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 881..932
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1254..1299
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 602..607
FT /note="Missing (in Ref. 1; AAN46880 and 3; AAL83292)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1557 AA; 173750 MW; 6B7D25CE60CA8D4B CRC64;
MEPTINPPVN LPPPVPSRSN LSLNYSNNNT NNTNNTNNTN DFKKKLQFQL QSSPSSPSSP
SPSIIYKKTP AVNNNNNNNN NNNHINNGNV NGIGPGGGNS YISSPQSSPI HTSSNGISYT
VTTNSPPLLP VDNSSGYNNN LNSTFVETLS SSSPSPIKTT TTPPPPVPSK SKSKSSEKLS
VKLLKSNSKP SLNDLYQQQQ QQSNPNSPTT PPSNCNIESI QPPSSSSSST TPSTSPQLPA
IYSKYSKISL PQLPLPPFLP PSPLVQSTSS PSFSSLILPL PSSPLPPPPL TIPNKVPPLP
MRLPPPPPPQ QLDQMYSNNN QQQQQQQQQQ QNNESNSTTT SEGGLTPESE SKLYEIASQP
PSTPRLTHES KVIPSEIELL IKFYPSPSLS SGSLLTIPST IEKSFIFSPC STVDEILSII
IPNFQFHNGL KCFAKHTFNN EWEATHNDPA AASSSIIDDH ESFALFNQKS PTIPLHKEKT
ILELSLHDRD VLILKTVSLV FTLVKVQIPH FTENSLSATA TVKFNQFTSI RSIIRKLYLK
YHNNVDISRH GIFLINDNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
NNNNNNNNNN LHQQQDELSS SIQLEEEELF STYNINSNSN LVFRTISNQE NDILNSSQKI
LNLKILISST FSRNNTLNLM FDPRDSVSKA IKVTGLRTGL LDSLNKCGFY LTPSEDDDEG
FWMDEELTLE MYNLKNHTFL SFKERCKKYT ILIKMGGASN GNGNGDLTWR KCTFKFDQFT
KVSTLFNILI NNENIKNPKD YHLVVKSTGT SLEKHRYLWS YDIKSPYEIE FKEYPNKLLI
FNPQNGEKNF VYVDFNEPIK DVCARLSQTF SSPIDFGSIS TNNNNNNNNN NNINSNSNGS
SGAGGANSNA LTSSTNSAST TSSSTPTTPE LPPQVNHVRE RSYTFKRLGQ LNNTIDSRKS
LKDQGVLPND ALMLEVIQDD SIATNNNNNN NNNNSNTTTT IKDKKINRNS LPSSTTMVFE
DLVKDGTILS EERQINIWDE PADSNGNIIY SKTVTNNLNA EIDAATLNKL IIRLTNPVFH
DLTFMKTFLM TYSSYTTTTT LLKKLFERFQ VPTHIDERER LSAQLRVANV IKYWVEHHYE
DFCHESTKLM VDFVDTHMMI AFPTLGVQIR NCILKRTCGF KSELVRTRSN GALTSPRTNL
SLSFTNLNNS SVGSIANTSS STSTSSTSSL LSSTNSISIS SSLSLSFNSG INSPSISQNT
PSSPSLIPSS PRPITSSSSV SSSTLLKSPL SQQAKSRIPE TKTKGFANPR NLFDFDDEEI
ARQLTLYDFQ LYTAIKPTEF LNQAWNKPSM ASRKSPTILK IISRFNDISL WVVSLILEPD
RVKTRAKRLK RIISIADELR KLNNYNTCIA VISGINNSAI LRLKYTRGLL SKKYLDILEN
LEKEMSCEGS YKNYRDKLKN SDPPVVPYIG VYLTDLTFIE EGNPNIIRGN LINFAKYYLI
YRVISEIQQY QWTEYQLNVA PIIQTFIRDV SISSTSDDLY HLSLLKEPRN ALKSDIF
