ID   GEFL_DICDI              Reviewed;        2356 AA.
AC   B0M0P8; Q8SSN3;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Ras guanine nucleotide exchange factor L;
DE   AltName: Full=RasGEF domain-containing protein L;
GN   Name=gefL; Synonyms=rasGEFL; ORFNames=DDB_G0276371;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=AX4;
RX   PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA   Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA   Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT   "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT   roles.";
RL   Genome Biol. 6:R68.1-R68.12(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during development.
CC       {ECO:0000269|PubMed:16086850}.
CC   -!- DISRUPTION PHENOTYPE: Poor in phototaxis assays.
CC       {ECO:0000269|PubMed:16086850}.
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DR   EMBL; AY160101; AAN46881.2; -; Genomic_DNA.
DR   EMBL; AAFI02000014; EAL69266.2; -; Genomic_DNA.
DR   RefSeq; XP_643190.2; XM_638098.2.
DR   AlphaFoldDB; B0M0P8; -.
DR   SMR; B0M0P8; -.
DR   STRING; 44689.DDB0185197; -.
DR   PaxDb; B0M0P8; -.
DR   PRIDE; B0M0P8; -.
DR   EnsemblProtists; EAL69266; EAL69266; DDB_G0276371.
DR   GeneID; 8620463; -.
DR   KEGG; ddi:DDB_G0276371; -.
DR   dictyBase; DDB_G0276371; gefL.
DR   eggNOG; KOG0619; Eukaryota.
DR   eggNOG; KOG3417; Eukaryota.
DR   HOGENOM; CLU_229636_0_0_1; -.
DR   InParanoid; B0M0P8; -.
DR   Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR   Reactome; R-DDI-171007; p38MAPK events.
DR   Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR   Reactome; R-DDI-354192; Integrin signaling.
DR   Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR   Reactome; R-DDI-392517; Rap1 signalling.
DR   Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR   Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR   Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR   PRO; PR:B0M0P8; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0003779; F:actin binding; IEA:InterPro.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR   GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR   GO; GO:0030036; P:actin cytoskeleton organization; IEA:InterPro.
DR   GO; GO:0046956; P:positive phototaxis; IMP:dictyBase.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR   GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR   GO; GO:0030587; P:sorocarp development; HMP:dictyBase.
DR   GO; GO:0043052; P:thermotaxis; IMP:dictyBase.
DR   CDD; cd00155; RasGEF; 1.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   Gene3D; 1.25.10.10; -; 1.
DR   Gene3D; 3.80.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR014768; GBD/FH3_dom.
DR   InterPro; IPR010473; GTPase-bd.
DR   InterPro; IPR001611; Leu-rich_rpt.
DR   InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   PANTHER; PTHR23113; PTHR23113; 1.
DR   Pfam; PF06371; Drf_GBD; 1.
DR   Pfam; PF13855; LRR_8; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   Pfam; PF00618; RasGEF_N; 1.
DR   SMART; SM00369; LRR_TYP; 9.
DR   SMART; SM00147; RasGEF; 1.
DR   SMART; SM00229; RasGEFN; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS51232; GBD_FH3; 1.
DR   PROSITE; PS51450; LRR; 12.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
PE   2: Evidence at transcript level;
KW   Guanine-nucleotide releasing factor; Leucine-rich repeat;
KW   Reference proteome; Repeat.
FT   CHAIN           1..2356
FT                   /note="Ras guanine nucleotide exchange factor L"
FT                   /id="PRO_0000384470"
FT   REPEAT          46..69
FT                   /note="LRR 1"
FT   REPEAT          72..93
FT                   /note="LRR 2"
FT   REPEAT          94..116
FT                   /note="LRR 3"
FT   REPEAT          117..138
FT                   /note="LRR 4"
FT   REPEAT          140..162
FT                   /note="LRR 5"
FT   REPEAT          163..184
FT                   /note="LRR 6"
FT   REPEAT          186..208
FT                   /note="LRR 7"
FT   REPEAT          209..230
FT                   /note="LRR 8"
FT   REPEAT          233..254
FT                   /note="LRR 9"
FT   REPEAT          256..277
FT                   /note="LRR 10"
FT   REPEAT          279..300
FT                   /note="LRR 11"
FT   REPEAT          302..323
FT                   /note="LRR 12"
FT   REPEAT          325..346
FT                   /note="LRR 13"
FT   DOMAIN          782..1354
FT                   /note="GBD/FH3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00579"
FT   DOMAIN          1956..2077
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT   DOMAIN          2112..2351
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT   REGION          1..28
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          391..425
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          498..575
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          599..630
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          644..672
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          686..771
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          865..900
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          963..994
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1008..1027
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1218..1244
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1755..1791
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1809..1831
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        865..899
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        971..994
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   2356 AA;  262822 MW;  1524A3FBC355B128 CRC64;
     MKSMSSGGIN KLFEGSLSKD GKTSRIEHQK IKQSGCPLLI KLMKDNGHIT NVIIKSCRLK
     VLPGEISSLG GHLKKLHIED NKIQEIPNLE QLEQLEELIL PNNDIAKFQV SISKLTTLRL
     LDLSGNQLGT IPVRLFSLVS MRELYLDENQ FSNFPSHLCE LQKLTTLGFS NNLLKSIPTQ
     IGQMIGLKKL ILSGNQMESI PMEISNLKSL THLDCSSNIL SSIPNELGNK LSQLSFLFLQ
     HNKLRSIPDE IGQCQSLVSL RLNNNSITLL PQSIGELENL QELYLQENRL NTLPSELGNC
     CSLKKLYLEF NKLIALPDRF KRLHCLNVLS LHDNLLDDLP NFLSLEFSQH LIRFTIHNNP
     FLRENGIDVI DSLQFLNSHL LSIQNFINNH DQQNDNSNNN NNNNNTTTTT TTTTTTTTTS
     TTNNNNTLFT DSYLSSASSS SFLNKSPSGL SLVGSFNGTI KKSISNSSNM NGSGSNGVGH
     GSGLTPKLNF KSLITSRDFS SDSDSDTDST SSFSTYSSST INTKRASISP RTSLTLSNSA
     SNDSVSAVAN GNGNNNNNNN NNNNNNNNSN NNNSGLVNSI SSFSLSSGQI NYYNISRARR
     GTHGNDQPHQ TISPSTSTEK LQQTVSTPRQ QSFFISVLNR RKSSLNNSQN GNNSNNNSNN
     NNNNSNNNIS SLSSSNFANS LYNPLMSSSP TSSPSSSYSN LHSSFNGTST PPFSSPSQSS
     PLSNSINGIH CNYSNNNNNN NNSNNNNNNN NNNNINNNNN NSNNNNNINT NGNNNSFNGI
     IMGSPIYKSF QSLYETLLDD LDYSKKRREE LLNLSVEEKW IFLQRIKPDC TNSLVSNGVY
     QLQHQISSND LKTLISSPPK LNSSQQLLSS SSSSSSLSSS STSATHSLQN KSLPSISVCP
     KSPRTHMKVL SASSLFTLED ITKLRQQLVS KPVSWVINFA ESTGIVSILS MITTYSQNLI
     NNVNKDQPQS QPQPQPQPQP RPQPQQPQPQ QPQPQQLLPL LSQLQPQQQQ QQQQQTQQPQ
     PQLQAQTKSD DHFIINECLG CIKLLVELEM QSLLLNDTAE IIFPLILSSQ VSTKRMTFEV
     LELICKIFSL GSTIVLESIK NYSKKINEPL TKVFSHITSP LDSEYPADLK VSCMSLINHL
     IGSNHLSLEA RIETRSKFLS NDILLIIKNL RIDGEGIETS SWKQLELEMK QFESMMVQDD
     NQTSNAEANE LVSNELSQSI DNNNNNNNNN NNNSSTNLVN NNNNNNINSN CNSIINGGNS
     SDSLINNSVG TGVGSIMGVG SFMGVNEIEY DSPRKEFDEL KSSNNNNSNS NSVNNFINNI
     TEPIMPLTPT EGKLVVIVSM QQTRQQAQLQ QQLQQQLEEE DDDEVPLSSS VATTASTTAN
     TTLIEPNHLP IQTREVIVSV QRKSQAGDVI RSLLASNSNL KELGEWGLFV NDSNSNNNNN
     NNNNNNNKLS TNVIGRFLKD DEYIFDQMTT SSTSGSLSSD DGATPRLMTR SIPRFKEYRL
     KMIPWKVRVS LEKVKEMGLV LNANNTANGQ FNESKFIAEA PMDPTMTCTG LVYHLIKKYL
     PNVNDRYCLE ADDFGLYLDT NAGGLMWGGG TGGGYWLEPL EKLHDYEIFK DPKSTVALKL
     RPKGVKLKFA DGTYEIFRLD LMSPTEKIFQ EISEKVIDSN NNINLSYYGI FVDRQQGGAN
     GNGISDSPIL VSQSSSTSLI EESKKQRKPE WLEKGLPLYH YKINSRCCLR FALRPSNITL
     LIDISLFKSF QQQQQQQQQQ QQQSTSSSSS SSSSSSVSSN NNSTNNSNNL IGSLNNSINT
     ISELDESIDD NGLSSSSSDK EDQISTPSTS NLLSKFQQVD ESISNLKFQL PQPIQSKQII
     VQLPLHLPLG EALETDENWI ETGIEREKCS FHFDSFDGIL MNQTQPLNTQ NFTEKNRICV
     LMADELEINS EQTNHNKTNI WDEPFDSSTI ICDPDNPGQI RAATLNKLLE QATNNIEKDR
     ELMNILLMTY MSFATSDILL DKLVERYNVP EHEKEQKSVV QLHTIVFLKN WLEQQAPQAA
     SGGGLEEKFL VRLSEFVDKQ ISLDGYTKIV PQLKKWIDSA LKEKRAYAMP EAARPNQLTT
     KLPSLTSSLL DDELFVAQQL TLREYETFKR IQAVEFLGQA WGKPKLQYKA PNLLKMIERF
     NKISTGVSTA ILSQPKIKTR VKLICRFIKI AQHCRDLNNY HLLTAFMAGI RNSNVVRLRL
     SWAKVPKKHL HILEDLEKIM SMEGSFKEFR TKMAETIPPC IPYLGVYLKD LTFIEEGNPD
     SINSLINWGK KKLIYNIISI IQRCQQVSYD FGNQNQSQVA QKSDLVLNYF DSLPIALDEL
     LYKISLNLEP RSNFNN