GEFM_DICDI
ID GEFM_DICDI Reviewed; 929 AA.
AC Q8SSQ3; Q555T3;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Ras guanine nucleotide exchange factor M;
DE AltName: Full=RasGEF domain-containing protein M;
GN Name=gefM; Synonyms=rasGEFM; ORFNames=DDB_G0274607;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AX4;
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=18590548; DOI=10.1186/1471-2180-8-109;
RA Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R.,
RA Ivens A., Martinez J.L., Escalante R.;
RT "Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common
RT and specific effects from PAO1 and PA14 strains.";
RL BMC Microbiol. 8:109-109(2008).
RN [5]
RP INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=18559084; DOI=10.1186/1471-2164-9-291;
RA Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B.,
RA Skelton J., Ivens A., Bozzaro S.;
RT "Genome-wide transcriptional changes induced by phagocytosis or growth on
RT bacteria in Dictyostelium.";
RL BMC Genomics 9:291-291(2008).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Expressed during development with a peak between
CC 12 and 14 hours of development. {ECO:0000269|PubMed:16086850}.
CC -!- INDUCTION: Up-regulated by Pseudomonas aeruginosa, PAO1 strain and PA14
CC strain infection and down-regulated by phagocytic stimuli.
CC {ECO:0000269|PubMed:18559084, ECO:0000269|PubMed:18590548}.
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DR EMBL; AY160102; AAN46882.1; -; Genomic_DNA.
DR EMBL; AAFI02000012; EAL70196.1; -; Genomic_DNA.
DR RefSeq; XP_643977.1; XM_638885.1.
DR AlphaFoldDB; Q8SSQ3; -.
DR SMR; Q8SSQ3; -.
DR STRING; 44689.DDB0191326; -.
DR PaxDb; Q8SSQ3; -.
DR EnsemblProtists; EAL70196; EAL70196; DDB_G0274607.
DR GeneID; 8619403; -.
DR KEGG; ddi:DDB_G0274607; -.
DR dictyBase; DDB_G0274607; gefM.
DR eggNOG; KOG3417; Eukaryota.
DR HOGENOM; CLU_314849_0_0_1; -.
DR InParanoid; Q8SSQ3; -.
DR OMA; LQDINFI; -.
DR Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR Reactome; R-DDI-171007; p38MAPK events.
DR Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR Reactome; R-DDI-354192; Integrin signaling.
DR Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR Reactome; R-DDI-392517; Rap1 signalling.
DR Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR PRO; PR:Q8SSQ3; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0043327; P:chemotaxis to cAMP; IMP:dictyBase.
DR GO; GO:0050850; P:positive regulation of calcium-mediated signaling; IMP:dictyBase.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR GO; GO:0106070; P:regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway; IMP:dictyBase.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 2: Evidence at transcript level;
KW Guanine-nucleotide releasing factor; Reference proteome.
FT CHAIN 1..929
FT /note="Ras guanine nucleotide exchange factor M"
FT /id="PRO_0000384471"
FT DOMAIN 390..528
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 676..911
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT REGION 1..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 169..316
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 540..583
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..38
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..85
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 169..286
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 297..316
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 929 AA; 101906 MW; 98D14BAFD7829AA7 CRC64;
MWQKPSLTKS MMNEVSSNSP KSPTLTSSPS TQSFANRVLP GRNLDGGGGG SNRLIFGGSG
GGSGGGSLPS SPVSSPVNPF NLNGGGGGKS SRRRGVTVAA GSEQVETRRK ILLGNSRPFR
RRARSMEILN SWDFIPYQKK PSDDEQGQVD QYIQGENDED LTSYESYITN NNTTTTTNTN
NSNNNNSNND GSGSGSGAGG SFIGTTTSAK TTSTTSTSAA TTTTTTTTSS SSSSPSSSSP
SSTSPTIASN NDNNNKIEPP QPSKIANSVP PLTLSQSQTQ QQQQQKVKPP PPSPRFSTNS
SGSQSPPVPP RSSKSLGLSN DFSISLSSSM SSNSLLISGS INTLPTSSSS SSSVGGSGAS
AASASISSSS SLSSSSLSVS KLIYENNEEN KFVVVSGPKD QLVEYLCNKN KLDFSFVNSF
ILSFRYFIEP EELFNHLMVK YETKVQTTSS IKVSKQFEEI QDRIRQNILI IISVWVDCNY
SDFEDNLSLH KRLFKLVQDT PHAVFLRDSI DRQKVSKIPI LDLYEKLKDE LLLSLSPRGS
LSGSGGISNN NNGSDLKNSN NGNNSSNNNN SSSNSSSSSS SSDIKNIKNS FFSLTTMMDW
LMNHLSIGYA TADSILKKLF KLKVIGSLGS GGGFTNISSS HTNSNSKSSK EELYFFTSTK
SIINKEIYSR SFMDYSPQDI AKQLTLIEFK LFQSVKMKEL YHKSWTISKS KFENSPNIMS
LITMSNKIAN WVATEVVTTP HPKKRVEVLK RFISVAEHCK KINNFNTLME VISGLSNSAV
SRLKETWKSL PTRYVNSFNS LQNFLKTDEN WKSYRQTLKT KETPCLPYLG LFLQDINFIE
DGNSNLSSEN DWVNFKKMNL LTSVFTEIQY FQRHPYFSFS THQNIQTYFE KDIVILPEKE
LYAFSKFIES PSNPLFRLSK SKNQSIFNI
