ID   GEFN_DICDI              Reviewed;        1138 AA.
AC   Q8IS12; Q553C1; Q869N0;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=Ras guanine nucleotide exchange factor N;
DE   AltName: Full=RasGEF domain-containing protein N;
GN   Name=gefN; Synonyms=rasGEFN; ORFNames=DDB_G0275703;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC   STRAIN=AX4;
RX   PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA   Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA   Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT   "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT   roles.";
RL   Genome Biol. 6:R68.1-R68.12(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [4]
RP   FUNCTION.
RX   PubMed=16234315; DOI=10.1093/bioinformatics/bti726;
RA   Booth E.O., Van Driessche N., Zhuchenko O., Kuspa A., Shaulsky G.;
RT   "Microarray phenotyping in Dictyostelium reveals a regulon of chemotaxis
RT   genes.";
RL   Bioinformatics 21:4371-4377(2005).
CC   -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP (By
CC       similarity). May play a role in chemotaxis. {ECO:0000250,
CC       ECO:0000269|PubMed:16234315}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during development; with a peak at 12
CC       hours. {ECO:0000269|PubMed:16086850}.
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DR   EMBL; AY160103; AAN46883.1; -; Genomic_DNA.
DR   EMBL; AAFI02000013; EAL69602.1; -; Genomic_DNA.
DR   RefSeq; XP_643499.1; XM_638407.1.
DR   AlphaFoldDB; Q8IS12; -.
DR   SMR; Q8IS12; -.
DR   STRING; 44689.DDB0191325; -.
DR   PaxDb; Q8IS12; -.
DR   PRIDE; Q8IS12; -.
DR   EnsemblProtists; EAL69602; EAL69602; DDB_G0275703.
DR   GeneID; 8620080; -.
DR   KEGG; ddi:DDB_G0275703; -.
DR   dictyBase; DDB_G0275703; gefN.
DR   eggNOG; KOG3417; Eukaryota.
DR   HOGENOM; CLU_278158_0_0_1; -.
DR   InParanoid; Q8IS12; -.
DR   OMA; NIALNCW; -.
DR   Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR   Reactome; R-DDI-171007; p38MAPK events.
DR   Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR   Reactome; R-DDI-354192; Integrin signaling.
DR   Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR   Reactome; R-DDI-392517; Rap1 signalling.
DR   Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR   Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR   Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR   PRO; PR:Q8IS12; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR   GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR   GO; GO:1903013; P:response to differentiation-inducing factor 1; HDA:dictyBase.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   Gene3D; 3.80.10.10; -; 1.
DR   InterPro; IPR001611; Leu-rich_rpt.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   PANTHER; PTHR23113; PTHR23113; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   PROSITE; PS51450; LRR; 2.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
PE   2: Evidence at transcript level;
KW   Guanine-nucleotide releasing factor; Leucine-rich repeat;
KW   Reference proteome; Repeat.
FT   CHAIN           1..1138
FT                   /note="Ras guanine nucleotide exchange factor N"
FT                   /id="PRO_0000384472"
FT   REPEAT          1..16
FT                   /note="LRR 1"
FT   REPEAT          18..39
FT                   /note="LRR 2"
FT   REPEAT          43..64
FT                   /note="LRR 3"
FT   DOMAIN          733..855
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT   DOMAIN          891..1118
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT   REGION          126..180
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          239..301
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          319..360
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          389..411
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          473..540
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          601..643
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          660..680
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        142..162
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        484..499
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        500..540
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1138 AA;  125574 MW;  D07BC283C05D10EF CRC64;
     MSYNSLTTID IDITQLLHLK SVNLRSNKLG NSSGVSYFGS MDTLQKLNLK ENYLTELPST
     FYLLKLSSLK LDTFTPLYQQ QQQQQNQLQR PIINSQSTSN ESLIHEINRS FENLSMSEPV
     SPRIAALKNE KKGPPKPLPK TKGLHSSSSN INTSNNITPI LMSPIHDGNK KSPSIISNNN
     NNNNSNSNII TSNGTVIPQL VFKNEPTGGR LRSFTLDADH ISPGRELVST GSSDEILYFN
     SEGGCENSDS DEPIQPLSAG RSRAQTISGK QPSIINNNNN NNSGGGSGNN NNSGGGGGSR
     KAFQNLIKSA KQIGSNIKNK THHINGHSSS SQSNSTTNTP SISSTPYPTS TIKTNVSDTN
     TPVNYNGQQQ ISKVIQRVSG EIDPLYSGID SPRTLERRNS SRDDIPISPP QGCMTPIKRL
     GSVEGDEISA IFSSSTDANM DVSQDYSSST PTVNSMSYQS SFLDTFDNQS TVGSPTFYQL
     PVSSQPPQHP PPPPPIKDNN QPKLNQSQNL INTNSNSVTT TNNSSQTTTT TTTTTTNNNN
     VLNLEKLHKL DFAEDINGDE RCSNPLYSKK KLAGLLFPNK HHQPSALKTA KIRFKSSKRL
     ATTVNSNSSN NLMMSNSPLS SSSMNLLQQS NSPQYSGTPT SGSFQNQSVL SATLMSSPSM
     SDVFSGGNSQ GGGSSLSQSG SITPVAPLSA VQGSPIGNSG SVNNIYLEGD DFIRDLMQSP
     IINQEIEFTS EDGVPKVKNI TLKMLTSLLT HEKGQSNELN GIFFDTYLLF TSIPLVIEHL
     ESRFLNGKNH IVKQKVITFS QKWVEVCWSD FKEEHIELLL KFLNDCSEQI EKLVTGSFAL
     RTSLNLLLNI INMKRDGTYI TEEEETFEQP APIPDFIQLP NCVDINLMDL RPHEIARQLT
     ILDHELLTQI TKQQMLDYAV HQTNSPSIVK VTDRFNYLVL WVSTEIVLSL TIEKRIETIF
     KFVNIALNCW YLKNFNSAIA IIAAMSKPSI EKLKQTWGFI RNTKANAPIQ ELKELILPTN
     VARLRKIMDS VEAPFIPYLG AYFSHSIAIH AGNKSIVNEQ YINMQKYDMM GKIIRSITVA
     QEKKYNLTPV GVLQKFLTDS FVLTEKQLYS EASKIEEKGD SYITETTKFG SIFKKKDK