GEFO_DICDI
ID GEFO_DICDI Reviewed; 944 AA.
AC Q8IS11; Q54GB4;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Ras guanine nucleotide exchange factor O;
DE AltName: Full=RasGEF domain-containing protein O;
GN Name=gefO; Synonyms=rasGEFO; ORFNames=DDB_G0290267;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AX4;
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Faintly expressed during development.
CC {ECO:0000269|PubMed:16086850}.
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DR EMBL; AY160104; AAN46884.1; -; Genomic_DNA.
DR EMBL; AAFI02000162; EAL62260.1; -; Genomic_DNA.
DR RefSeq; XP_635769.1; XM_630677.1.
DR AlphaFoldDB; Q8IS11; -.
DR STRING; 44689.DDB0191357; -.
DR PaxDb; Q8IS11; -.
DR PRIDE; Q8IS11; -.
DR EnsemblProtists; EAL62260; EAL62260; DDB_G0290267.
DR GeneID; 8627573; -.
DR KEGG; ddi:DDB_G0290267; -.
DR dictyBase; DDB_G0290267; gefO.
DR eggNOG; KOG3417; Eukaryota.
DR HOGENOM; CLU_311340_0_0_1; -.
DR InParanoid; Q8IS11; -.
DR OMA; NIWDEPK; -.
DR Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR Reactome; R-DDI-171007; p38MAPK events.
DR Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR Reactome; R-DDI-354192; Integrin signaling.
DR Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR Reactome; R-DDI-392517; Rap1 signalling.
DR Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR PRO; PR:Q8IS11; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR027370; Znf-RING_LisH.
DR InterPro; IPR000315; Znf_B-box.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR Pfam; PF00643; zf-B_box; 1.
DR Pfam; PF13445; zf-RING_UBOX; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
DR PROSITE; PS50119; ZF_BBOX; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Guanine-nucleotide releasing factor; Metal-binding;
KW Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..944
FT /note="Ras guanine nucleotide exchange factor O"
FT /id="PRO_0000384473"
FT DOMAIN 402..528
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 727..944
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT ZN_FING 16..54
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT ZN_FING 152..192
FT /note="B box-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT REGION 80..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 530..562
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 587..623
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 644..670
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 200..234
FT /evidence="ECO:0000255"
FT COILED 271..303
FT /evidence="ECO:0000255"
FT BINDING 157
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 160
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 179
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
FT BINDING 184
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00024"
SQ SEQUENCE 944 AA; 106970 MW; 4B4EB64370497128 CRC64;
MTTNSKSLNL LQSLTCGICQ NLFKDPNTLI PCGHAFCLDC LTTNASIKNC IQCKVEYTTY
IPNHPLKQMI DCLDQSSNNN NSNNNSNGEN TNNNNNIINN ERSSFNGSNS NSIIGFIEGL
SDSTSNGRRF SQQDGLRYSS GLLDGSNSGG SNNIRYCMEH YEHYYAFCND CQAPVCPSCL
LTTHNRHGMI PLTKDSIATK MKEYRDIVQS FKTKMSQYKD NITLYQKEIE LLDSTFLQCK
QSIQLMISNL HKVLKSRETY LLKEISSIHY ASHMELTDRS STLENEISEM EKLIGNGTDK
FKDATEILNN QNLKFEFLEQ FHHSRTQSKK NQNQDGLKPL FKTDLLFYKA NNERVTEMIN
GTLGNISLLT FPLDDIGEVN IWDEPKENIC IEKVRTNSNG IEEFEVKYGS LNKLIERLCL
PNCYDDNYVN IFLLTYHSFC SSKKLLKKLI ERFTIPEDLE AHGLTLASIH EIHMKIRSVL
VKWINEYSPK FDQDTIHLFQ NFNCRMQSEY TSIQEIENLL LNSNENSPSI TSSQSLSSQS
LYSQTNNNNN NNNNNSNNLQ PTIQTNQTLS SNINNITISN LNNSNLTNNG TCKIQNSPPK
NYQQSNYSSI FNGPSSSSSS SPSSPILSLN SLALINGNDN LVFESPLNSP RNNNNNNNNN
SPRSSSFGAS SALKFNSNNN SLNINSNMGG PYFPSPSSPT TNNIPNINHF SSRILSSSKN
LEFNDIDEFE IAKQLTLIEF ENFGRIKPID LLTCVDLKHK TPHITNIMER FHNISTWVST
TIVRGENLKN RVKIVNKFIK IAEHLKNLNN FNSLTAILVA IQRSTVTKKD LVKQSVKIIT
DLEKLMSSDD SYSTYRTRLA QCSPPCVPYI SIYLQDIMDL EKKNPSNIIV QTSSNKTQEF
INFTRRSLIS KVILDLASYQ RFGYSTILPI SNIQEYLNVH IDEL
