GEFP_DICDI
ID GEFP_DICDI Reviewed; 1502 AA.
AC Q54TK8; Q8IS10;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Ras guanine nucleotide exchange factor P;
DE AltName: Full=RasGEF domain-containing protein P;
GN Name=gefP; Synonyms=rasGEFP; ORFNames=DDB_G0281573;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AX4;
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Faintly expressed during development.
CC {ECO:0000269|PubMed:16086850}.
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DR EMBL; AY160105; AAN46885.1; -; Genomic_DNA.
DR EMBL; AAFI02000042; EAL66548.1; -; Genomic_DNA.
DR RefSeq; XP_640583.1; XM_635491.1.
DR AlphaFoldDB; Q54TK8; -.
DR SMR; Q54TK8; -.
DR STRING; 44689.DDB0215009; -.
DR PaxDb; Q54TK8; -.
DR PRIDE; Q54TK8; -.
DR EnsemblProtists; EAL66548; EAL66548; DDB_G0281573.
DR GeneID; 8623191; -.
DR KEGG; ddi:DDB_G0281573; -.
DR dictyBase; DDB_G0281573; gefP.
DR eggNOG; KOG3417; Eukaryota.
DR HOGENOM; CLU_248639_0_0_1; -.
DR InParanoid; Q54TK8; -.
DR OMA; CNFLKRW; -.
DR Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR Reactome; R-DDI-171007; p38MAPK events.
DR Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR Reactome; R-DDI-354192; Integrin signaling.
DR Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR Reactome; R-DDI-392517; Rap1 signalling.
DR Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR PRO; PR:Q54TK8; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR CDD; cd00014; CH; 1.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.418.10; -; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; PTHR23113; 3.
DR Pfam; PF00307; CH; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00033; CH; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF47576; SSF47576; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS50021; CH; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Guanine-nucleotide releasing factor; Reference proteome.
FT CHAIN 1..1502
FT /note="Ras guanine nucleotide exchange factor P"
FT /id="PRO_0000384474"
FT DOMAIN 84..187
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT DOMAIN 1102..1249
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 1267..1498
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT REGION 216..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 325..436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 456..534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 589..935
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 287..328
FT /evidence="ECO:0000255"
FT COILED 451..515
FT /evidence="ECO:0000255"
FT COILED 1032..1076
FT /evidence="ECO:0000255"
FT COMPBIAS 325..349
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 353..367
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 387..436
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 456..510
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 589..738
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 750..773
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 783..798
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 810..935
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 109
FT /note="S -> P (in Ref. 1; AAN46885)"
FT /evidence="ECO:0000305"
FT CONFLICT 185
FT /note="F -> C (in Ref. 1; AAN46885)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1502 AA; 168949 MW; ABFBC89FDE90845C CRC64;
MNDSTQILDE SGDFSFLSLN ENKSRSRSTS TCSINSVEII DGGSCDSSGD DDKNNSYLDE
EKKRILRKTY LYFNANNKKT QLNFIIDNQV LDWIFNQTKT KISKPICESL KNGVALCKII
NLIKPNTIKK INLNSSIFSY RENLTNFTKG CESIGMTEDM SRFQELVFED KESAVLQLLY
SLMKFSEKIL PNTISTSPPS QLNICKSPVL LKKAAAQSSS SSPTTSTSAS TSTSTSPSSS
NESIAKPILS ALSSSSSTLF SAAPTPTSTN TVPYKFIGHS KSKLFGQQQQ QQQQQQQQQQ
QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQPP PQTSTCTTTQ PTTTTTEEHK LAPPPPLIVP
NTSSLPPPQP TTSSIIKKAA PAPLKKPSPA NTSSNSLLNH LPPPPSSSST ASSAINTPIS
TPSTSKSNSF QKPNNNFQKP IQIAHVEPII EENEIEDNTN NNNNNNNNNN NNNNNNNNNN
NNNNNNNNNN NTNDNINNNN KNNNNNQNEN EDEVKPVHSP PKVRPPLPSR VGRGQLLRSS
SEEIQFDIEN ASVSEAPSPV MTMNEDYSIV TDANSLTNHY NGANEYTTTT TAATTNLPNE
SSSRSNSASS NGDQNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNLQLP AKINSEPNNQ
YNTISTSSPS TTGTILESPN GSSIFSQPLN NNNNINNENN NSNNSNSLVT SSSPPLSLPA
YSANGSPNCP QGKKQRSGTK LRSFFGVKSK PESKSVSNFY GVGNSINQPP SNSSPKQSPV
PNNPPSVSQS FLNLSMNSKS SKKKKDNISP DINSNSNNNN NNNNNNNNNN NNNNNNNNNN
NNNNNNNNNN NNNLEEDENP LTMSNQSANS LKSSGNHFED DITNGNNNIS QNQNQNQNQN
QNQTQNQNQN QNQNHISHSN SISSGNLNNH VNQNNNVNEE SVDSELMKAL SKVEFLFKDR
KVLKERINFT YPEKYLKFFV NMDELIQIDN STTFLTESLL SKRPSQQLEL CHQEERRFLS
EIVTLQNVLN VVEENKNLIT RTEEMQKMID SLMKEKKELI NEKNTLASML AKTKQQQQQQ
SNDTVSQRKM VDDGIVYNYN HGKYEIKGGT TEKLIELLYN SEFIGSDYFE TFLLTYRSFA
SPKRVMDVLT KTYNENYSVE GNATDDVEKR LELDQFNKQR KDTHLKICNF LKRWVEKHFY
DFDQDLLQEF NTFIANCRVI NHQDFLQKTL NKKLIPLSSS ELKLVFSTPT PAPILPKTPI
TCFEDMDPAE IARQLTLIEF NLFKNIANKE FLSLSWQKQD KEKRSPNLLK MIYRFNEVSN
WVTSTIVKET TNIKKRASYL KRFIKLAEEL RKLNNFNGVF VIVSGLHSAS VNRLKNTWAE
ISKQQQKQFE EFVALTSPQS SFASYRLELR QSTGASIPYL GVHLSDLTFV EEGNQDKLEN
GYTNFSKCRL IAEQIKAIQE FQQEPYNLRE VEEMIPFITH KAVPESECFN LSLICEPRES
VN
