GEFQ_DICDI
ID GEFQ_DICDI Reviewed; 1298 AA.
AC Q86G47; Q54H67;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 106.
DE RecName: Full=Ras guanine nucleotide exchange factor Q;
DE AltName: Full=RasGEF domain-containing protein Q;
GN Name=gefQ; Synonyms=rasGEFQ; ORFNames=DDB_G0289665;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AX4;
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION.
RX PubMed=16234315; DOI=10.1093/bioinformatics/bti726;
RA Booth E.O., Van Driessche N., Zhuchenko O., Kuspa A., Shaulsky G.;
RT "Microarray phenotyping in Dictyostelium reveals a regulon of chemotaxis
RT genes.";
RL Bioinformatics 21:4371-4377(2005).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP (By
CC similarity). Seems to play a role in chemotaxis. {ECO:0000250,
CC ECO:0000269|PubMed:16234315}.
CC -!- DEVELOPMENTAL STAGE: Expressed during development; with a peak between
CC 8-12 hours of development. {ECO:0000269|PubMed:16086850}.
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DR EMBL; AY254473; AAP13089.1; -; Genomic_DNA.
DR EMBL; AAFI02000148; EAL62544.1; -; Genomic_DNA.
DR RefSeq; XP_636051.1; XM_630959.1.
DR AlphaFoldDB; Q86G47; -.
DR SMR; Q86G47; -.
DR STRING; 44689.DDB0191356; -.
DR PaxDb; Q86G47; -.
DR PRIDE; Q86G47; -.
DR EnsemblProtists; EAL62544; EAL62544; DDB_G0289665.
DR GeneID; 8627260; -.
DR KEGG; ddi:DDB_G0289665; -.
DR dictyBase; DDB_G0289665; gefQ.
DR eggNOG; KOG3417; Eukaryota.
DR HOGENOM; CLU_261712_0_0_1; -.
DR InParanoid; Q86G47; -.
DR OMA; FLYTYHY; -.
DR Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR Reactome; R-DDI-171007; p38MAPK events.
DR Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR Reactome; R-DDI-354192; Integrin signaling.
DR Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR Reactome; R-DDI-392517; Rap1 signalling.
DR Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR PRO; PR:Q86G47; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005938; C:cell cortex; IDA:dictyBase.
DR GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR GO; GO:0005634; C:nucleus; IDA:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0051015; F:actin filament binding; IDA:dictyBase.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:dictyBase.
DR GO; GO:0045159; F:myosin II binding; IPI:dictyBase.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IMP:dictyBase.
DR GO; GO:0007265; P:Ras protein signal transduction; IDA:dictyBase.
DR GO; GO:0043520; P:regulation of myosin II filament assembly; IDA:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR000591; DEP_dom.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00610; DEP; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00049; DEP; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS50186; DEP; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Guanine-nucleotide releasing factor; Reference proteome.
FT CHAIN 1..1298
FT /note="Ras guanine nucleotide exchange factor Q"
FT /id="PRO_0000384475"
FT DOMAIN 550..689
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 723..801
FT /note="DEP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00066"
FT DOMAIN 964..1193
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 46..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 211..271
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 314..384
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 459..533
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 781..864
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 884..920
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1214..1267
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 352..389
FT /evidence="ECO:0000255"
SQ SEQUENCE 1298 AA; 143833 MW; 7BC6CF0E1E1BDB58 CRC64;
MDINNNIDNI TNSNNNIEKN NNINNFDNNN NNNNILYISS SESNNNNNIN SSNSSSNNTN
NSNNSSISNN NNNNNNNNNN NNNISIEGNT ANNSDIKVGT SLTNSIKSFK KLFNNQKSNN
NNNNLQVNHF QQSQSLPPSS NTSPTFSSTS PIIIVSDNTN IESLDLEINS NNSGSIGSNN
SGDNNNINMN YKSKSLSLNY SILRPLCDTD NISNNNNNNN NNSNNSNNNN NMSSSDNNNN
SNSNNTTNGG QLKRNNSSNN NNRYSIGGPL KMSNSSDFND YLLNNNILYN INQPNRKTPP
PNLMSVLKNS FTQYTPPSTT LTSPTSTRNP NRLSTNLSNV TNISSSTSSS SSSLNANNNT
NNNNQQLQQQ QQQQQQQQLQ QQQQLTKSYS VSNLMRSQSS SSSSQSPLIQ QSDQVTFDYD
YIYRRFLRSR SELTLPSWDN AQYEGDFDTE IQDTLTRALS NGNLPSDNKR QSLHLSTEST
TSNNNNNNNN NNNNNNNNNN NKSKTDDTTN SATTTTTTTT TTTTTTTTNS TTTTTQLNNG
KNLIGLVYND KDEVIAGERD KIIGLFTDIS KVTGTQFLEE FLFTYHYYMT SVDLLENLKG
RFNNPLMDQA AQSISILEEN HDKVKIEQLS THIRLRVINV IKKWIENHGY AFATDINLYQ
MLVDFIDGDV MSFSQRWGQY LKKAINDSGC LLRYIAVNVK EASSKQLREI ISLDNLDAIS
KLMSQSLQLK ERKKGLKVIK NSFTGNEAVE WLINKFNLDS KDEALETLSK MLQSNLIFHH
SKSGSSFSPS SSSSSTTTTT TTSSSSSSSN TTTANMASSS SATDNSSSGG VVTSSSSSSS
SSSSSPSNGN GTTAITSTSL PNSISSSNST SILTSLVVNS NSSGIANGSS TPSIPSSVTS
PLTNSKSTLN STPSSSSNSF TFKDKSTSIY YFIFENINFP EPIVPKTFFS SPNSTFSITF
LDIHPVEIAR QLTLIDYELF KKLSPTDFYH TAWSKPDAKE KVPNLINFIN RSNTVSYWVA
TEILSSSNIK HRVSVLKRFI TIAEILRKFH NWNTLTGILM GLNLGSIQRL KKTWESIDKK
QLDSLQNLIN LTSERLNYSN YRKEMSTPTY PCLPFMAVYL KDLYFIEENP DYLENGYINF
EKMKMISKVL IEIKRYQTEQ YWLKKIDAIE NILKSVVLTD KDLYKASHMI EQPQRTMTVG
SNLKAQSFLT TTTTTTTTNN LNNNNNNNNP NNNNNNNNNS ANNKSSPSPS PSSSPITSSP
ISSLTINNNN TISSNLSAST DSSINTKKKS SFLMFGKK
