ID   GEFS_DICDI              Reviewed;        1043 AA.
AC   Q8SSW7; Q55AH0;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Ras guanine nucleotide exchange factor S;
DE   AltName: Full=RasGEF domain-containing protein S;
GN   Name=gefS; Synonyms=rasGEFS; ORFNames=DDB_G0271868;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC   STRAIN=AX4;
RX   PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA   Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA   Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT   "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT   roles.";
RL   Genome Biol. 6:R68.1-R68.12(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during development; especially between
CC       12-14 hours of development. {ECO:0000269|PubMed:16086850}.
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DR   EMBL; AY160107; AAN46887.1; -; Genomic_DNA.
DR   EMBL; AAFI02000007; EAL71513.1; -; Genomic_DNA.
DR   RefSeq; XP_645439.1; XM_640347.1.
DR   AlphaFoldDB; Q8SSW7; -.
DR   SMR; Q8SSW7; -.
DR   STRING; 44689.DDB0191324; -.
DR   PaxDb; Q8SSW7; -.
DR   EnsemblProtists; EAL71513; EAL71513; DDB_G0271868.
DR   GeneID; 8618179; -.
DR   KEGG; ddi:DDB_G0271868; -.
DR   dictyBase; DDB_G0271868; gefS.
DR   eggNOG; KOG3417; Eukaryota.
DR   HOGENOM; CLU_292303_0_0_1; -.
DR   InParanoid; Q8SSW7; -.
DR   OMA; VVLKYWI; -.
DR   Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR   Reactome; R-DDI-171007; p38MAPK events.
DR   Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR   Reactome; R-DDI-354192; Integrin signaling.
DR   Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR   Reactome; R-DDI-392517; Rap1 signalling.
DR   Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR   Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR   Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR   PRO; PR:Q8SSW7; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR   GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR   CDD; cd00155; RasGEF; 1.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   Gene3D; 2.30.29.30; -; 1.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   PANTHER; PTHR23113; PTHR23113; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   Pfam; PF00618; RasGEF_N; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SMART; SM00229; RasGEFN; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   PROSITE; PS00720; RASGEF; 1.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Guanine-nucleotide releasing factor; Reference proteome.
FT   CHAIN           1..1043
FT                   /note="Ras guanine nucleotide exchange factor S"
FT                   /id="PRO_0000384477"
FT   DOMAIN          645..768
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT   DOMAIN          803..1043
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT   REGION          135..160
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          245..316
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          109..142
FT                   /evidence="ECO:0000255"
FT   COILED          404..434
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        250..316
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1043 AA;  116900 MW;  9508E6248C3E7F0E CRC64;
     MGEVSLIGIT AILCQKTDGF WKNIDDSLCR LEVLKSNIDS KFRLVAISTD NRCIFNKWVR
     TNSEITKCSD LFLEIKQVSS QSSNVEYFGV NFAYKPEVDK FYACFSKCIE TLQTQRRQST
     LNIQALQINN ELQQQLQQQQ QLPPIDQLPP PPTSTSTSTI SNANISISNS ISYDSLHQMA
     SGITLEDLPP PPPSASSLIL NNNTPLDLTS LPPIDFSNLP PPPTGLMTSR SSLTEANGLE
     IKNKLSPLLN GGASGLSSSP PPTTESKNQL NSSSGSNNSS SAPHLTDLFL PTNNNNSSGN
     QSASNSSPTS QYHQSQPNLQ QYGTAPLTAS SKLLQQQQQQ QQQQQQNIIM TTTTTTTTTI
     NIKTGSSSDS FLKTASGRRS IRVRHSTNQA ADQQKKTMGR KDGLAVSLQN VEGLQNIAEN
     LEDETLNLLD LVNEQVVTPE ISNNAHLNQS LMKIFEHLQV LFILTGQAST HPGGKLITQV
     VNKLGRGPQA SEFFSGKSVD GGYQWYNLDE VKDSVIGIHN LLALKKNLIT AIAHLSTCVR
     VLGLQASLEI DWMSRNKSSE TEKIVVQLAC LTRELISSMS RLLAATVTYC YVCSSIAFIR
     NQQHSASADH NQASIQNRIR SPSTIDTINI WDELKTIKTI PTVPKDGSIL KVTLNQLVLM
     LTSETSYDSK FLKTFITTYQ SFASPGVLFT KLIERFYVPE WYSTVPTKIS TIQQKVIVVL
     KYWIENQSSD FDQDVIDQIY FFINNLANSN EGYSELSRLL RGLLDKMIQD REVKFELLFQ
     MPPRISFEED SILSPIELFS EWSAQSIAQQ LTLIDFSIFK DLEARELLNQ NFNKPKLKYK
     SPTIMRMISK STQFSFWVAY VILMEPKKEK RIKIFEKFCE VGKYLLKMNN FNSLMGLNAG
     LNLTCVHRLK KTKKKLSSSA ISILTELERI FSSKKSFKNY RDHLSTVQLP CIPYLGFNLT
     DITFIEEGNT DNISSTDPAA ANIGPLINFK KRELLYQAWA DLSRFQETPY TFQPEEPLNT
     FLLNFPILDD KELYDLSIAL EPK