GEFV_DICDI
ID GEFV_DICDI Reviewed; 1982 AA.
AC Q55FD8;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Ras guanine nucleotide exchange factor V;
DE AltName: Full=RasGEF domain-containing protein V;
GN Name=gefV; Synonyms=rasGEFV; ORFNames=DDB_G0268578;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP DEVELOPMENTAL STAGE.
RC STRAIN=AX4;
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed during development; with a peak at 12
CC hours of development. {ECO:0000269|PubMed:16086850}.
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DR EMBL; AAFI02000003; EAL73742.1; -; Genomic_DNA.
DR RefSeq; XP_647595.1; XM_642503.1.
DR AlphaFoldDB; Q55FD8; -.
DR SMR; Q55FD8; -.
DR STRING; 44689.DDB0231998; -.
DR PaxDb; Q55FD8; -.
DR EnsemblProtists; EAL73742; EAL73742; DDB_G0268578.
DR GeneID; 8616407; -.
DR KEGG; ddi:DDB_G0268578; -.
DR dictyBase; DDB_G0268578; gefV.
DR eggNOG; KOG0619; Eukaryota.
DR eggNOG; KOG3417; Eukaryota.
DR HOGENOM; CLU_229636_0_0_1; -.
DR InParanoid; Q55FD8; -.
DR OMA; HLKINIE; -.
DR Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR Reactome; R-DDI-171007; p38MAPK events.
DR Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR Reactome; R-DDI-354192; Integrin signaling.
DR Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR Reactome; R-DDI-392517; Rap1 signalling.
DR Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR PRO; PR:Q55FD8; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0003779; F:actin binding; IEA:InterPro.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR GO; GO:0030036; P:actin cytoskeleton organization; IEA:InterPro.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR GO; GO:0030587; P:sorocarp development; HMP:dictyBase.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR Gene3D; 3.80.10.10; -; 3.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR014768; GBD/FH3_dom.
DR InterPro; IPR010473; GTPase-bd.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF06371; Drf_GBD; 1.
DR Pfam; PF13855; LRR_8; 2.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM01140; Drf_GBD; 1.
DR SMART; SM00369; LRR_TYP; 12.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS51232; GBD_FH3; 1.
DR PROSITE; PS51450; LRR; 13.
DR PROSITE; PS00720; RASGEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Guanine-nucleotide releasing factor; Leucine-rich repeat;
KW Membrane; Reference proteome; Repeat; Transmembrane; Transmembrane helix.
FT CHAIN 1..1982
FT /note="Ras guanine nucleotide exchange factor V"
FT /id="PRO_0000384478"
FT TOPO_DOM 1..1831
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1832..1848
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1849..1982
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 1..26
FT /note="LRR 1"
FT REPEAT 47..68
FT /note="LRR 2"
FT REPEAT 69..94
FT /note="LRR 3"
FT REPEAT 105..128
FT /note="LRR 4"
FT REPEAT 130..151
FT /note="LRR 5"
FT REPEAT 164..187
FT /note="LRR 6"
FT REPEAT 188..210
FT /note="LRR 7"
FT REPEAT 212..234
FT /note="LRR 8"
FT REPEAT 236..257
FT /note="LRR 9"
FT REPEAT 261..284
FT /note="LRR 10"
FT REPEAT 286..307
FT /note="LRR 11"
FT REPEAT 308..330
FT /note="LRR 12"
FT REPEAT 331..352
FT /note="LRR 13"
FT REPEAT 354..376
FT /note="LRR 14"
FT REPEAT 378..399
FT /note="LRR 15"
FT REPEAT 443..466
FT /note="LRR 16"
FT REPEAT 592..615
FT /note="LRR 17"
FT REPEAT 657..684
FT /note="LRR 18"
FT REPEAT 773..796
FT /note="LRR 19"
FT DOMAIN 832..1236
FT /note="GBD/FH3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00579"
FT REPEAT 979..1003
FT /note="LRR 20"
FT REPEAT 1075..1100
FT /note="LRR 21"
FT REPEAT 1239..1263
FT /note="LRR 22"
FT DOMAIN 1595..1717
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT REPEAT 1689..1712
FT /note="LRR 23"
FT DOMAIN 1747..1974
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT REPEAT 1865..1888
FT /note="LRR 24"
FT REPEAT 1917..1941
FT /note="LRR 25"
FT REGION 414..436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 457..532
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 615..654
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 756..778
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 807..829
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 515..567
FT /evidence="ECO:0000255"
FT COMPBIAS 457..502
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1982 AA; 221935 MW; 8157A79FB9501DA5 CRC64;
MGNINSICLN NNGKYRIDSI SCSLEGCKLN SKELVENMVA LVDKYPLTQL LIIRECKLKQ
IPVNITNLSN LSELILSDNK LSQLPWSLPP PFKPNTLLSM ASITNENLVR LDLSNNRFTE
FPSSVFVLPN LKQLILCNNQ LTNMNVTLCG GTSNNNGAHQ PHIACQLEEL KLSNNNFTIF
PSIIGDQLTT LKSLDLSGNT ITSLPNSFSN LVSLTSLNLK SNKFTCFPPS LCTLDKLVHL
NLSCNQILVS PSDHTLGVSL LPSLEKLELQ HNRFAHFPMD ILEIVSLRVL KLQDNDIDKI
PDKIGNLLNL NELFLSENKI TQLPSTIGEL INLRKLYLEY NKIGSLPQEF SKLSKLNILI
LHNNDLKFVP DQLHSLSQLL RLSLDENQLS SSDQKLIKSE GSLALIKRKN KLNYGSTMNG
TGTTSSSGSA STSTHGSINK TTDILLSSVT LNNSILNQIN NNNNNNNNNN NNNNNNNNSS
NNNSGTNSLS STPNGTGKSK KKLTHLTISR SLFRGNSSNL ESEKEDFINK KQQQQQQQQQ
QQQQQQQQQQ QQQQQQQQQQ QQQQQLGSTA SPSTASPITS HITVNSTVLD FEDDIQKMQL
GLEALSNLET SIGSNNSGGG DSMNGSGGNI NNSGGSGSGC GTISGSTTKQ RRGSVFLPPT
NAFKLSPNVV SSSYNTLPAS VMSGLLQQQN LENGGVGSGS GSGSPIISAN SICNSNSSSS
NSLLKPPIIN TTNLSTSPNL LSQSSTSFLN LPPLNQSSTN SFLPPQHQHH HHHSNLSQSL
SINNLAHRLP TSLSSSSLSS NNTYDSLQFQ QQQQHHHHNH NNHQNSNQPL ATIVPSFSKF
KNSFDQLLEE QDFSKKKRET LQKLSKEQKW NLLLQYRNST LNMLNMKEHS NNSNNNQSIK
NSTIIASTTT TTTTNGESLD GSSIQQQITK NSNFQSSRFY IDNLTSKTDI SLVKEVHDFL
KESGPEVSIF IGMGGIESLL GILQFNLDNA QLQFDEEKVG YCLLCLKILV ECTAKSVITT
NNSLNLVTMF LTSASKYIID IIIDIYDELL RLQVIGVSVV LDSLINFQKV KGLSSPYVPL
VALLRNPIIE MPTKTTVFKI INFLIYSFSV LEERFSVICS LLKVGILEVI GMFRALPHTS
YSLRFELDLF EEVLDSDESE LVKKIGRKEV LKRMSNEDND LSKQSSKQSM DFVRVVMVSA
GYGDLKIDLD ERTSYANVID FIQSNYQIKY SIDRYGLFVP AIGSLVLDGS ILQSSGSQSR
WVDPSKSLID NGLSNEIVEF KMKPMQIQVL QSVVVVVATN HQKEDIIKEF TIDPQLKCFE
IVQYLRTRLD SSSPAFQRKI SVLTEDEDED YESDIYGVYC FSSLESYDQG SWYPLNSRFL
DFNPTNTPTI IDVKLMERPL RVLFSGTEKL MKFNPYVPVS ELIKEIKNEI HLKINIEDYG
ISYVPNVSSS NLSSLIIEET WLDPTKTLLD HNDMKCSVVQ FKFKPRTVSV MVLVESGIEI
PTTDTPYQFV NTNSISEIIE KICTPHQLNP LSHSLYISDC QGQKIKLLSR QNCLRDQQVN
NGDHLIITSK QKDLPSDFNV WDEPNDSSET ITYSKDRRVS SVTLNKLIEK LTSPEYTQDS
EMKTIFLSSY RSIITPSVLL SKLMERHTIP TLVDKERGKL IQRKTRLLIK SWLELETTKN
REEVITLLVQ NMPAQSIEDD GLKELTELFV DLSTKSYSSI LPIVIGELPK CKIPKIIDSY
VTFADMDEIE IARQLSLLTF PLYSRIETSE LLNQRWSKPG GGPNIMSVVG LFNKISNWVS
FTIVNQPKLR DRAVVYGKMV KIANAFYELR NYHLLMAIIS GLNASPVLRL KYTKGKLSKN
LKDNLDTLEE LMSTQSSMKN YRADLAAASP PAIPFMGFHL SDLVFIDEGN QQLCDSRINF
KKLEMYKKTI ATLQNFSLFP YQFTPVPIIQ NYFLDYKIVA DKPIYEMSLS AEPRNAERYD
IQ
