GEFW_DICDI
ID GEFW_DICDI Reviewed; 1172 AA.
AC Q55GH9;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Ras guanine nucleotide exchange factor W;
DE AltName: Full=RasGEF domain-containing protein W;
GN Name=gefW; Synonyms=rasGEFW; ORFNames=DDB_G0267666;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP DEVELOPMENTAL STAGE.
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed during development; especially at 12
CC hours of development. {ECO:0000269|PubMed:16086850}.
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DR EMBL; AAFI02000003; EAL73286.1; -; Genomic_DNA.
DR RefSeq; XP_647205.1; XM_642113.1.
DR AlphaFoldDB; Q55GH9; -.
DR SMR; Q55GH9; -.
DR STRING; 44689.DDB0231999; -.
DR PaxDb; Q55GH9; -.
DR EnsemblProtists; EAL73286; EAL73286; DDB_G0267666.
DR GeneID; 8616009; -.
DR KEGG; ddi:DDB_G0267666; -.
DR dictyBase; DDB_G0267666; gefW.
DR eggNOG; KOG3417; Eukaryota.
DR HOGENOM; CLU_274004_0_0_1; -.
DR InParanoid; Q55GH9; -.
DR OMA; IVNDAHW; -.
DR Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR Reactome; R-DDI-171007; p38MAPK events.
DR Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR Reactome; R-DDI-354192; Integrin signaling.
DR Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR Reactome; R-DDI-392517; Rap1 signalling.
DR Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR PRO; PR:Q55GH9; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; -; 1.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR019804; Ras_G-nucl-exch_fac_CS.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; PTHR23113; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF48366; SSF48366; 1.
DR PROSITE; PS00720; RASGEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Guanine-nucleotide releasing factor; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..1172
FT /note="Ras guanine nucleotide exchange factor W"
FT /id="PRO_0000384479"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 432..452
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 545..565
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 765..903
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 938..1170
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT REGION 34..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 138..162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 186..246
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 666..702
FT /evidence="ECO:0000255"
SQ SEQUENCE 1172 AA; 133857 MW; 563C535CFAC101AC CRC64;
MAERVKILKP GLTNRSRSAS IDMIVMDEIA KNNPIYTNDN SNNNNNDETS SNISGSNSIQ
NLSLNNLNNL SGGGGGSNSN SVNNTISERN PDSPRFRSNT GNLQTMMMVS NGNIIGSIQS
PSPPISPRAQ MTAPNLSKFL DQHTPPPSPK PEHLSGHLRI QQQHHSGIYR NLSFSSVNSS
TSFGDFKRST NLFQPPQSQS QQQQQLQLQQ QQQQSMPNLS LGNNINSNNN NNNGSENNDI
SMEIKDNTSG IDTSVKSFSP RKKRKKENKI IRRIRRWMTW VISSNIWLTI MIISIIFVLF
IDDIIGASGS PSSNAIDYSI MAVKCSIIGF FSIDIILNLF CFQQEYFPGT IVFWLDLISL
ISIVTDIHHF QNYYANLVLS ITVNTRIIRI CGSFIRISLI STLYNRFLRK QTLPSEGLEV
EASKLGDKLI RLTTNKIVLL ALAVLFATQL LVYESDTPTQ YIQSTISSFE YLSSTYGLQS
PEFTGVFNNY QLNNEKLLFT QISNQKIFNL PDKINSLQER SILKYNYLNS IIWIDNSYYI
KYSCILHLCL TVFVILVLIT INLLIVNDAH WLVINPLENV LTIVKLLSKQ NSMIKQGGGG
SGIVGGFSNN TINSNSNLNS NSNLNSNSNS NLVRNRVESI GNKSDTADTT SSSGIDNEEP
DEADFLLGML NEIDDSLQAA KEKVEEESIQ NSILKKDIED LYVEKYILQV HLRSIVRKIN
FHDPIGHYLK NRQLLLMQSN ATFNTLEQDS EDDIRFKLND DGLPDLNVIQ YATIDKLIEK
LTMAEAHDLK FANVFLLTYR KFLSPVELME RLTIRFCVTP TMELPEKLLA SKEQVEQWRK
TKQEQIRTSV FNTIKLWIGI YNWDFYENPD LYELFNNLVN KIMPFCKMEK HATYIDSIHK
RKMATYIQDP PYIPIEPLTQ EEIAETMVLE DRLLFNFDIS DIAIQITLME FDLFKNIKSK
EFLNLAWTHK TEKTRLSPNI VRFIEHFNSV SFWLQTCIVK SGKIKERVAV LKKVIALADV
FVQLNNYYGA MEVLSSLESS AVSRLHKTWE QVPQSSIQSL QSLQKLLSPQ ENFKNYRERI
SKCGSCCIPY IGLYLSDLTF IHEGNPDYYQ QSQLINFSKL REVAITINTI KQFQNIFYYY
EKNQNIRSQM NFKSPGADII WKMSLSCEQR NK
