ID   GEFY_DICDI              Reviewed;        1508 AA.
AC   Q552M5; Q86JC7;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 2.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Ras guanine nucleotide exchange factor Y;
DE   AltName: Full=RasGEF domain-containing protein Y;
GN   Name=gefY; Synonyms=rasGEFY; ORFNames=DDB_G0276019;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA   Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA   Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT   "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT   roles.";
RL   Genome Biol. 6:R68.1-R68.12(2005).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=16512674; DOI=10.1021/pr050350q;
RA   Reinders Y., Schulz I., Graef R., Sickmann A.;
RT   "Identification of novel centrosomal proteins in Dictyostelium discoideum
RT   by comparative proteomic approaches.";
RL   J. Proteome Res. 5:589-598(2006).
CC   -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during development; with a peak between
CC       8-12 hours of development. {ECO:0000269|PubMed:16086850}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAL69466.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AAFI02000013; EAL69466.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_643387.1; XM_638295.1.
DR   AlphaFoldDB; Q552M5; -.
DR   SMR; Q552M5; -.
DR   IntAct; Q552M5; 1.
DR   STRING; 44689.DDB0238867; -.
DR   PaxDb; Q552M5; -.
DR   EnsemblProtists; EAL69466; EAL69466; DDB_G0276019.
DR   GeneID; 8620272; -.
DR   KEGG; ddi:DDB_G0276019; -.
DR   dictyBase; DDB_G0276019; gefY.
DR   eggNOG; KOG3417; Eukaryota.
DR   InParanoid; Q552M5; -.
DR   Reactome; R-DDI-1169092; Activation of RAS in B cells.
DR   Reactome; R-DDI-171007; p38MAPK events.
DR   Reactome; R-DDI-193648; NRAGE signals death through JNK.
DR   Reactome; R-DDI-354192; Integrin signaling.
DR   Reactome; R-DDI-381676; Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
DR   Reactome; R-DDI-392517; Rap1 signalling.
DR   Reactome; R-DDI-5673001; RAF/MAP kinase cascade.
DR   Reactome; R-DDI-9013148; CDC42 GTPase cycle.
DR   Reactome; R-DDI-9013149; RAC1 GTPase cycle.
DR   PRO; PR:Q552M5; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR   GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR   CDD; cd00155; RasGEF; 1.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   PANTHER; PTHR23113; PTHR23113; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   Pfam; PF00618; RasGEF_N; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SMART; SM00229; RasGEFN; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Guanine-nucleotide releasing factor; Reference proteome.
FT   CHAIN           1..1508
FT                   /note="Ras guanine nucleotide exchange factor Y"
FT                   /id="PRO_0000384480"
FT   DOMAIN          1074..1234
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT   DOMAIN          1278..1508
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT   REGION          1..73
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          128..182
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          197..272
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          396..517
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          565..593
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          606..727
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          831..1021
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1153..1172
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          659..686
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        7..24
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        32..73
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        128..155
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        164..182
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        197..225
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        232..247
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        257..272
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        409..462
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        488..508
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        567..593
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        606..694
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        695..726
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        831..887
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        945..981
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        982..996
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        997..1021
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1508 AA;  166169 MW;  F2DD24E2C3A00128 CRC64;
     MIIIGVKRNI NNSNNSNSNN NSNKKKDKIM SHNNNNTITG KNTNEIMNNN NNSNNNNNNN
     NNNNNNNNNN NNEEVKTEPI QQILKRLNSK NKIEVGATPN DIPTSIPSVK NLGKKYDSKA
     ETHLLNQKVL SSPSKEVNSL KKSTNGTNTI PSSSIPSSPP SSPPSSSSLS SDRTSQDIPK
     SVNGLTASIV AKFSKNIDNN TTNNNSNNNN NSSLSTPIQS PRDSLETNPI KDEESEESEE
     SEESKEEEEE LKMGIKTTKT TSETIESSYS TSQLLKNDLI QEKGDDNNNI QEPQQPKYPT
     QTSFITDKSK RLSTPPINGT SISIGNNNNN NNNYINGNSG INTIPRNFQS HRVAGDWKDR
     KLNTLFTQGT VGGNGQNGTV RKNLIGTREL STSSGILQCK DDSSSKDQDF EFNNSAGSSG
     NSSASNSNRN SIAFSSSNHF SSESSQSAPT LPIYNPSQYT SPSTPQSPSP SPSPPLISSK
     SIYINKPPPP HFNQQTNFSV SPTKSPSNEK QQQEDEDVKH LVRAFTNRKL NVHHAERKSK
     ISVQDAEWIV KKFFHDTDRQ SKFVLSENRT KDLNQPSSQF SPSTSPSTNS IPTNLLLSNK
     RLSTSLPNIN TNINNSSNNT SSSSSSSSTT SSPSPLTTSS STSVLPPPLS SSSSSQLTES
     LKTRIEENNN NNNNKNINNN NNNNNNNGRN SIHDSQEMTE LEDPHSSPSY RHLEEVGKKK
     YVSAEPSRRP GIKTPLFLNV PTLAMPTQFH QIPPERVSSN LFSVSVEENS YPDNPLLFNQ
     QIINRVANDK DLSYLFFPVL KSSTKITSST LNNIIPINKL NNQIHNNNNN NVIISNNTGN
     NNNNNNSSKN NNTVKGNKKQ QNKSSSSSQN NSPPSDFSLS VSPSPCSSLT PSPSPSPSSP
     LIESSMFTKD LSPPPPAAAA IVLQQPVATT NNKSTDKDSR KSLWSSMKSS KPPISTSQPS
     TIQDTISTSP PSTSTSPTSN SPPTSISPPP PSITTGSSPP STAGTSPPND NGNNNNNSNK
     ELTISIPITN VNVHESNIVV ASTPRSGRDL ATIVAEEFKD RTDIVFCTQH LSMNRIKVRS
     ASLDAMIDLL TNHRLSQPDL VESFLLTYKT FTSPLAVLTK LIERYEETDN INNNNNNNND
     DLIISTRTTA TITNEDEDNS NSNSNSNKTN KNSSRTIKLG VLSIIKCWVD RHHYDFERNK
     ALLSAIVAFL EGPVIDDGME KVSNIILKII DRKANEAEAR RTGTALVMHT STSHCKFPPS
     IPPSLSKPDQ IPTLQNFDDL EIARQLTLIE HEAYSMVKPN ECINLAFSKS DKEIRAPNII
     NIIKRSNLLP LWVATEIVQE ERLTKRANII KKFISIADQC KNLNNFNAVM EILSGLNLTP
     VFRLKKTWET LPRKYLATFR HLNSLMAPKF NFKVYRDVLH TKNLPCLPFL GVYLTDLTFL
     EEGSFDQAEN GLINIVKRTQ IANIIQEIQQ YQQLSYSFAP VPIIKDFLLQ IGGLQERALY
     KQSKIIEP