ID   GEMA_BPD10              Reviewed;         183 AA.
AC   P24796; C9DGM3;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   11-JUN-2014, sequence version 2.
DT   23-FEB-2022, entry version 55.
DE   RecName: Full=GemA protein;
DE   AltName: Full=Gene product 16;
DE            Short=gp16;
GN   Name=gemA;
OS   Escherichia phage D108 (Bacteriophage D108).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Muvirus; unclassified Muvirus.
OX   NCBI_TaxID=665033;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Kropinski A.M., Villegas A., Lingohr E.J.;
RL   Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-26.
RX   PubMed=2147059; DOI=10.1093/nar/18.21.6458;
RA   Pato M.L., Banerjee M., Wagonner B.T.;
RT   "Sequence of gene E15 of bacteriophage D108 and comparison with phage Mu.";
RL   Nucleic Acids Res. 18:6458-6458(1990).
CC   -!- FUNCTION: Early protein responsible for decreasing host DNA gyrase
CC       activity. Promotes DNA relaxation of bacterial host genome. Modulates
CC       the expression of various host genes probably controlled by
CC       supercoiling of their promoters. Host genes affected include DNA
CC       replication and cell division determinants (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: This protein is constitively expressed from the Pgem
CC       promoter unlike most other early proteins which are expressed under the
CC       control of the Pe promoter. Its expression seems to escape repression
CC       by repressor protein c and thus occurs throughout latency (Probable).
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the mulikevirus gemA protein family.
CC       {ECO:0000305}.
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DR   EMBL; X54298; CAA38198.1; -; Genomic_DNA.
DR   EMBL; GQ357916; ACV50274.1; -; Genomic_DNA.
DR   PIR; S12146; S12146.
DR   RefSeq; YP_003335763.1; NC_013594.1.
DR   GeneID; 8658826; -.
DR   KEGG; vg:8658826; -.
DR   Proteomes; UP000000320; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0060153; P:modulation by virus of host cell cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR009363; Phage_Mu_Gp16.
DR   Pfam; PF06252; DUF1018; 1.
PE   3: Inferred from homology;
KW   Early protein; Host cytoplasm; Host-virus interaction;
KW   Modulation of host cell cycle by virus; Reference proteome.
FT   CHAIN           1..183
FT                   /note="GemA protein"
FT                   /id="PRO_0000077788"
SQ   SEQUENCE   183 AA;  21218 MW;  6F971FE19BA7FAC8 CRC64;
     MSRTSLIKLI HVARRELQLD DDTYRAFLMQ KTGKISCREL TVTQLEQVLD AMKERGFKKL
     NKYPRRRFKE HVTPREKVYK IWQQMAEDGF ITDGGDVALD KYVQRLTAKR NGGQGVSTLA
     WCHGESLQVV LETLKQWHIR CIREAFSRHG LPLPVSPSGR ELRGYDAMTA AYAHARKTRR
     MAQ