GEMC1_MOUSE
ID GEMC1_MOUSE Reviewed; 333 AA.
AC Q3URY2; Q3TUK3; Q5RJG4;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Geminin coiled-coil domain-containing protein 1;
GN Name=Gmnc; Synonyms=Gemc1, Gm606;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Fetal head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Head;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Regulator of DNA replication. Promotes initiation of
CC chromosomal DNA replication by mediating TOPBP1- and CDK2-dependent
CC recruitment of CDC45L onto replication origins (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Note=Associates with
CC chromatin during pre-replication complex (pre-RC) formation.
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q3URY2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3URY2-2; Sequence=VSP_034993;
CC Name=3;
CC IsoId=Q3URY2-3; Sequence=VSP_034992;
CC -!- PTM: Highly phosphorylated by CDK2; stimulates initiation of DNA
CC replication. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the GEMC1 family. {ECO:0000305}.
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DR EMBL; AK141059; BAE24555.1; -; mRNA.
DR EMBL; AK160719; BAE35968.1; -; mRNA.
DR EMBL; BC086669; AAH86669.1; -; mRNA.
DR CCDS; CCDS37304.1; -. [Q3URY2-2]
DR CCDS; CCDS88906.1; -. [Q3URY2-1]
DR CCDS; CCDS88907.1; -. [Q3URY2-3]
DR RefSeq; NP_001013783.1; NM_001013761.2. [Q3URY2-2]
DR RefSeq; NP_001272845.1; NM_001285916.1. [Q3URY2-1]
DR RefSeq; NP_001272847.1; NM_001285918.1. [Q3URY2-3]
DR AlphaFoldDB; Q3URY2; -.
DR SMR; Q3URY2; -.
DR STRING; 10090.ENSMUSP00000087269; -.
DR iPTMnet; Q3URY2; -.
DR PhosphoSitePlus; Q3URY2; -.
DR PaxDb; Q3URY2; -.
DR PRIDE; Q3URY2; -.
DR ProteomicsDB; 266790; -. [Q3URY2-1]
DR ProteomicsDB; 266791; -. [Q3URY2-2]
DR ProteomicsDB; 266792; -. [Q3URY2-3]
DR Antibodypedia; 49140; 65 antibodies from 18 providers.
DR Ensembl; ENSMUST00000089832; ENSMUSP00000087269; ENSMUSG00000068428. [Q3URY2-2]
DR Ensembl; ENSMUST00000231299; ENSMUSP00000156264; ENSMUSG00000068428. [Q3URY2-3]
DR Ensembl; ENSMUST00000231969; ENSMUSP00000156070; ENSMUSG00000068428. [Q3URY2-1]
DR GeneID; 239789; -.
DR KEGG; mmu:239789; -.
DR UCSC; uc007yvi.1; mouse. [Q3URY2-1]
DR UCSC; uc007yvj.2; mouse. [Q3URY2-2]
DR UCSC; uc007yvk.2; mouse. [Q3URY2-3]
DR CTD; 647309; -.
DR MGI; MGI:2685452; Gmnc.
DR VEuPathDB; HostDB:ENSMUSG00000068428; -.
DR eggNOG; ENOG502RE7T; Eukaryota.
DR GeneTree; ENSGT00940000153270; -.
DR HOGENOM; CLU_071972_0_0_1; -.
DR InParanoid; Q3URY2; -.
DR OMA; QEANCEN; -.
DR OrthoDB; 966768at2759; -.
DR PhylomeDB; Q3URY2; -.
DR TreeFam; TF344118; -.
DR BioGRID-ORCS; 239789; 0 hits in 70 CRISPR screens.
DR ChiTaRS; Gmnc; mouse.
DR PRO; PR:Q3URY2; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q3URY2; protein.
DR Bgee; ENSMUSG00000068428; Expressed in vascular system and 26 other tissues.
DR ExpressionAtlas; Q3URY2; baseline and differential.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0060271; P:cilium assembly; IMP:MGI.
DR GO; GO:0006260; P:DNA replication; IMP:UniProtKB.
DR GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR GO; GO:0045786; P:negative regulation of cell cycle; IBA:GO_Central.
DR GO; GO:0008156; P:negative regulation of DNA replication; IBA:GO_Central.
DR InterPro; IPR029700; GEMC1.
DR PANTHER; PTHR13372:SF2; PTHR13372:SF2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell cycle; Coiled coil; DNA replication; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..333
FT /note="Geminin coiled-coil domain-containing protein 1"
FT /id="PRO_0000346426"
FT REGION 145..165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 83..118
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..59
FT /note="MNSILPCQDQYFVGGQSYNCPYSTTTSESGVDVSKETWVSFWAAGLLDNTEL
FT QQAPQVL -> MRITDWATRGYGRCCVP (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_034992"
FT VAR_SEQ 1
FT /note="M -> MEDAVFQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_034993"
SQ SEQUENCE 333 AA; 37758 MW; F8CD1B325167E82D CRC64;
MNSILPCQDQ YFVGGQSYNC PYSTTTSESG VDVSKETWVS FWAAGLLDNT ELQQAPQVLE
SPSELSFPVL DSCSWEEAQL SSQLYRNKQL QDTLLQKEEE LARLHEENNH LRQYLNSTLV
KRLEEKAKKL LSSDEFSKVF GKLRKEKRKP KEHRHSPAEI PQFKTAKRNL STEFSNCEEQ
PGPHVDPWVL QTLGLKDLNT IDDTLPANYS ATTSHPRAVP SISSLFVHDA NGYANVPRDL
HLDYVGQGTH GSHTTSIHQE DCHSFPRLSN PPVRVQTLPY HTADVSPNKT EMAFSTSLSP
HCNVKTHSFH QGQAFVRRDE EGGWKFTWVP KQT
