ID   GEMI8_BOVIN             Reviewed;         234 AA.
AC   Q1LZ79;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Gem-associated protein 8;
DE            Short=Gemin-8;
GN   Name=GEMIN8;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal pons;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The SMN complex catalyzes the assembly of small nuclear
CC       ribonucleoproteins (snRNPs), the building blocks of the spliceosome,
CC       and thereby plays an important role in the splicing of cellular pre-
CC       mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins
CC       SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in
CC       a heptameric protein ring on the Sm site of the small nuclear RNA to
CC       form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1,
CC       SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm
CC       complex by the chaperone CLNS1A that controls the assembly of the core
CC       snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm
CC       proteins from CLNS1A forming an intermediate. Binding of snRNA inside
CC       5Sm triggers eviction of the SMN complex, thereby allowing binding of
CC       SNRPD3 and SNRPB to complete assembly of the core snRNP (By
CC       similarity). {ECO:0000250|UniProtKB:Q9NWZ8}.
CC   -!- SUBUNIT: Part of the core SMN complex that contains SMN1, GEMIN2/SIP1,
CC       DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP
CC       (By similarity). Part of the SMN-Sm complex that contains SMN1,
CC       GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8,
CC       STRAP/UNRIP and the Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE,
CC       SNRPF and SNRPG (By similarity). Interacts with GEMIN6; the interaction
CC       is direct (By similarity). Interacts with GEMIN7; the interaction is
CC       direct (By similarity). Interacts with SMN1; the interaction is direct
CC       (By similarity). Interacts with GEMIN4; the interaction is direct (By
CC       similarity). {ECO:0000250|UniProtKB:Q9NWZ8}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, gem {ECO:0000250|UniProtKB:Q9NWZ8}.
CC       Cytoplasm {ECO:0000250|UniProtKB:Q9NWZ8}. Note=Found in nuclear bodies
CC       called gems (Gemini of Cajal bodies) that are often in proximity to
CC       Cajal (coiled) bodies. Also found in the cytoplasm (By similarity).
CC       {ECO:0000250}.
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DR   EMBL; BC116157; AAI16158.1; -; mRNA.
DR   RefSeq; NP_001069199.1; NM_001075731.2.
DR   AlphaFoldDB; Q1LZ79; -.
DR   SMR; Q1LZ79; -.
DR   STRING; 9913.ENSBTAP00000013582; -.
DR   PaxDb; Q1LZ79; -.
DR   PRIDE; Q1LZ79; -.
DR   Ensembl; ENSBTAT00000013582; ENSBTAP00000013582; ENSBTAG00000010289.
DR   GeneID; 515968; -.
DR   KEGG; bta:515968; -.
DR   CTD; 54960; -.
DR   VEuPathDB; HostDB:ENSBTAG00000010289; -.
DR   VGNC; VGNC:29321; GEMIN8.
DR   eggNOG; ENOG502S7KA; Eukaryota.
DR   GeneTree; ENSGT00390000013608; -.
DR   HOGENOM; CLU_073610_0_0_1; -.
DR   InParanoid; Q1LZ79; -.
DR   OMA; RAYARYW; -.
DR   OrthoDB; 1593621at2759; -.
DR   TreeFam; TF328591; -.
DR   Reactome; R-BTA-191859; snRNP Assembly.
DR   Proteomes; UP000009136; Chromosome X.
DR   Bgee; ENSBTAG00000010289; Expressed in choroid plexus and 105 other tissues.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0097504; C:Gemini of coiled bodies; IEA:UniProtKB-SubCell.
DR   GO; GO:0032797; C:SMN complex; ISS:UniProtKB.
DR   GO; GO:0034719; C:SMN-Sm protein complex; ISS:UniProtKB.
DR   GO; GO:0000387; P:spliceosomal snRNP assembly; ISS:UniProtKB.
DR   InterPro; IPR034754; GEMIN8.
DR   PANTHER; PTHR16238; PTHR16238; 1.
DR   Pfam; PF15348; GEMIN8; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Cytoplasm; mRNA processing; mRNA splicing; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..234
FT                   /note="Gem-associated protein 8"
FT                   /id="PRO_0000271400"
FT   REGION          60..116
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          130..153
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        64..81
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        82..114
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   234 AA;  26744 MW;  10EC6382C00E13E4 CRC64;
     MEAKSESTQA SESWSSHPVY ARYWQHYHQA MAWMRSHQSA YQKAVESVVT SPWYYPATDL
     AQSPAAKGGT SPKSRSKSPS ASGDACRRRS RPGKPGPQRR STEKPARFAE DNDSESDDCG
     IVCDLSNMVI TDELRQYFAE TEQHREELRR QHQLDAARLK DYVNADHGLY CDKRGRSTLP
     PTERPGERRQ AEMKRLYGAS AAKIQAMETA MQLSFDKHCD RKQPKYWPVI PLKF