ID   GEMI_CAEEL              Reviewed;         180 AA.
AC   G5EEM1;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=Geminin homolog {ECO:0000305};
GN   Name=gmn-1 {ECO:0000303|PubMed:15811859, ECO:0000312|WormBase:Y75B8A.17};
GN   ORFNames=Y75B8A.17 {ECO:0000312|WormBase:Y75B8A.17};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|EMBL:BAD98298.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, INTERACTION WITH CDT-1;
RP   NOB-1 AND CEH-32, AND DISRUPTION PHENOTYPE.
RX   PubMed=15811859; DOI=10.1074/jbc.C500070200;
RA   Yanagi K., Mizuno T., Tsuyama T., Tada S., Iida Y., Sugimoto A., Eki T.,
RA   Enomoto T., Hanaoka F.;
RT   "Caenorhabditis elegans geminin homologue participates in cell cycle
RT   regulation and germ line development.";
RL   J. Biol. Chem. 280:19689-19694(2005).
RN   [2] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: Inhibits DNA replication by binding to the DNA replication
CC       licensing factor cdt-1 (PubMed:15811859). Its interaction with cdt-1
CC       prevents the cdt-1 loading of the mini-chromosome maintenance (MCM)
CC       complex onto DNA and therefore DNA replication licencing
CC       (PubMed:15811859). {ECO:0000269|PubMed:15811859}.
CC   -!- SUBUNIT: Homodimer (PubMed:15811859). Interacts with cdt-1; the
CC       interaction most likely inhibits the ability of cdt-1 to load the mini-
CC       chromosome maintenance (MCM) complex onto DNA and therefore reduces DNA
CC       replication licensing activity (PubMed:15811859). Interacts with nob-1
CC       and ceh-32 (PubMed:15811859). {ECO:0000269|PubMed:15811859}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:O75496}. Nucleus
CC       {ECO:0000250|UniProtKB:O75496}. Note=Mainly cytoplasmic, but can be re-
CC       localized to the nucleus. {ECO:0000250|UniProtKB:O75496}.
CC   -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in sterility in
CC       20% of animals (PubMed:15811859). Sterile animals have severe oogenesis
CC       defects and partially differentiated germ cells accumulate in the
CC       proximal arm of the gonad (PubMed:15811859). Furthermore, germ nuclei
CC       in the distal region of the gonad are enlarged, and their nucleoli are
CC       misshapen (PubMed:15811859). Sterile animals also have mitotic defects
CC       whereby pairs of chromosome bridge, which is indicative of a defect in
CC       coordination between endoreduplication and the nuclear division cycle
CC       (PubMed:15811859). {ECO:0000269|PubMed:15811859}.
CC   -!- SIMILARITY: Belongs to the geminin family. {ECO:0000305}.
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DR   EMBL; AB190260; BAD98298.1; -; mRNA.
DR   EMBL; BX284603; CAA22084.1; -; Genomic_DNA.
DR   PIR; T27384; T27384.
DR   RefSeq; NP_499589.1; NM_067188.1.
DR   SMR; G5EEM1; -.
DR   IntAct; G5EEM1; 2.
DR   STRING; 6239.Y75B8A.17; -.
DR   EnsemblMetazoa; Y75B8A.17.1; Y75B8A.17.1; WBGene00013552.
DR   WormBase; Y75B8A.17; CE23028; WBGene00013552; gmn-1.
DR   eggNOG; ENOG502TEGT; Eukaryota.
DR   HOGENOM; CLU_1429173_0_0_1; -.
DR   OMA; ADRLQMD; -.
DR   OrthoDB; 1561671at2759; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00013552; Expressed in embryo and 4 other tissues.
DR   GO; GO:0140297; F:DNA-binding transcription factor binding; IPI:UniProtKB.
DR   GO; GO:0045786; P:negative regulation of cell cycle; IMP:UniProtKB.
DR   GO; GO:0008156; P:negative regulation of DNA replication; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Cytoplasm; DNA replication inhibitor; Nucleus; Reference proteome.
FT   CHAIN           1..180
FT                   /note="Geminin homolog"
FT                   /id="PRO_0000456082"
SQ   SEQUENCE   180 AA;  20197 MW;  943EA1232517DC2A CRC64;
     MSRIGLQQLN NSARNSPFGS EKATGTKQIP EPLKLTAQLK KYQPITPSPL ASATTITPVL
     SPFDVFCDDD QEAKNVETQM FEYGTVSTQT IINVPHVQPK ITEADLTSEK PTVNYLRVMA
     DRLQMDLDDE MDRNQRLVAE LGDLDEKMRK IDDDTEILLE VLADIDEEQQ TDEEIGTAQL