GEM_ARATH
ID GEM_ARATH Reviewed; 299 AA.
AC Q8S8F8; Q3EBW7; Q8GWY9; Q8LBI8;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=GLABRA2 expression modulator;
GN Name=GEM; OrderedLocusNames=At2g22475; ORFNames=F14M13.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBUNIT, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=17450124; DOI=10.1038/nature05763;
RA Caro E., Castellano M.M., Gutierrez C.;
RT "A chromatin link that couples cell division to root epidermis patterning
RT in Arabidopsis.";
RL Nature 447:213-217(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in the spatial control of cell division, patterning
CC and differentiation of Arabidopsis root epidermal cells. Could be part
CC of a complex that negatively modulates GLABRA2 and CAPRICE expression
CC via the maintenance of a repressor histone H3 epigenetics status of the
CC GL2 and CPC promoters. {ECO:0000269|PubMed:17450124}.
CC -!- SUBUNIT: Interacts with CDT1, a DNA replication protein and TTG1
CC (TRANSPARENT TESTA GLABRA1), a WD40-repeat protein involved in GLABRA2-
CC dependent cell fate decision. {ECO:0000269|PubMed:17450124}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8S8F8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8S8F8-2; Sequence=VSP_029568, VSP_029569;
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:17450124}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the GEM family. {ECO:0000305}.
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DR EMBL; EF490993; ABP35534.1; -; mRNA.
DR EMBL; AC006592; AAM15301.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07310.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07311.1; -; Genomic_DNA.
DR EMBL; AK118552; BAC43153.1; -; mRNA.
DR EMBL; AY087182; AAM64738.1; -; mRNA.
DR RefSeq; NP_565538.1; NM_127813.3. [Q8S8F8-1]
DR RefSeq; NP_973510.1; NM_201781.1. [Q8S8F8-2]
DR AlphaFoldDB; Q8S8F8; -.
DR BioGRID; 2133; 4.
DR STRING; 3702.AT2G22475.1; -.
DR iPTMnet; Q8S8F8; -.
DR PaxDb; Q8S8F8; -.
DR PRIDE; Q8S8F8; -.
DR ProteomicsDB; 220730; -. [Q8S8F8-1]
DR EnsemblPlants; AT2G22475.1; AT2G22475.1; AT2G22475. [Q8S8F8-1]
DR EnsemblPlants; AT2G22475.2; AT2G22475.2; AT2G22475. [Q8S8F8-2]
DR GeneID; 816780; -.
DR Gramene; AT2G22475.1; AT2G22475.1; AT2G22475. [Q8S8F8-1]
DR Gramene; AT2G22475.2; AT2G22475.2; AT2G22475. [Q8S8F8-2]
DR KEGG; ath:AT2G22475; -.
DR Araport; AT2G22475; -.
DR TAIR; locus:505006267; AT2G22475.
DR eggNOG; ENOG502QUT3; Eukaryota.
DR HOGENOM; CLU_063785_1_0_1; -.
DR InParanoid; Q8S8F8; -.
DR OMA; HCAIMST; -.
DR OrthoDB; 1201777at2759; -.
DR PhylomeDB; Q8S8F8; -.
DR PRO; PR:Q8S8F8; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8S8F8; baseline and differential.
DR Genevisible; Q8S8F8; AT.
DR GO; GO:0005773; C:vacuole; HDA:TAIR.
DR GO; GO:0051567; P:histone H3-K9 methylation; IDA:TAIR.
DR GO; GO:0010482; P:regulation of epidermal cell division; IMP:TAIR.
DR GO; GO:0048765; P:root hair cell differentiation; IMP:TAIR.
DR GO; GO:0010026; P:trichome differentiation; IMP:TAIR.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR037848; GEM-like.
DR InterPro; IPR004182; GRAM.
DR InterPro; IPR011993; PH-like_dom_sf.
DR PANTHER; PTHR31969; PTHR31969; 1.
DR Pfam; PF02893; GRAM; 1.
DR SMART; SM00568; GRAM; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Reference proteome.
FT CHAIN 1..299
FT /note="GLABRA2 expression modulator"
FT /id="PRO_0000311664"
FT DOMAIN 175..252
FT /note="GRAM"
FT REGION 1..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 53..112
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 239..248
FT /note="VIPLHQLKAV -> MCPTPFISEL (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_029568"
FT VAR_SEQ 249..299
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_029569"
FT CONFLICT 47
FT /note="E -> V (in Ref. 5; AAM64738)"
FT /evidence="ECO:0000305"
FT CONFLICT 146
FT /note="H -> Q (in Ref. 5; AAM64738)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="P -> L (in Ref. 4; BAC43153)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 299 AA; 32215 MW; FE56DBED9C40DA23 CRC64;
MEPPKGDTVV KTEVPVKDPS LSVVDSKTKG VEDANTEIAL SDEVEIETKG SDSTPVKAPS
RTSSGSKKSV HWSPELVSGS QEPDQKAASS SSAGSNPYIA RSPAETSDAS LKDTMETVKG
VLGRWGKRVA EAAKKTESLA GNTWQHLRTA PSFADAAMGR IAQSTKVFAE GGYEKIFRQT
FETDPEEQLL NSFACYLSTS AGPVMGVLYI SSAKLAYCSD NPLSYKNGDQ TEWSYYKVVI
PLHQLKAVNP SASIVNPAEK YIQVISVDNH EFWFMGFLNY DGAVTSLQDS LQAGALRSV
