GEM_HUMAN
ID GEM_HUMAN Reviewed; 296 AA.
AC P55040; B2RA31;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 204.
DE RecName: Full=GTP-binding protein GEM;
DE AltName: Full=GTP-binding mitogen-induced T-cell protein;
DE AltName: Full=RAS-like protein KIR;
GN Name=GEM; Synonyms=KIR;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Peripheral blood;
RX PubMed=7912851; DOI=10.1126/science.7912851;
RA Maguire J., Santoro T., Jensen P., Siebenlist U., Yewdell J., Kelly K.;
RT "Gem: an induced, immediate early protein belonging to the Ras family.";
RL Science 265:241-244(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7809057; DOI=10.1073/pnas.91.26.12448;
RA Cohen L., Mohr R., Chen Y.-Y., Huang M., Kato R., Dorin D., Tamanoi F.,
RA Goga A., Afar D., Rosenberg N., Witte O.;
RT "Transcriptional activation of a ras-like gene (kir) by oncogenic tyrosine
RT kinases.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:12448-12452(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Amygdala;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP INTERACTION WITH CALMODULIN.
RX PubMed=9115241; DOI=10.1074/jbc.272.18.11832;
RA Moyers J.S., Bilan P.J., Zhu J., Kahn C.R.;
RT "Rad and Rad-related GTPases interact with calmodulin and calmodulin-
RT dependent protein kinase II.";
RL J. Biol. Chem. 272:11832-11839(1997).
RN [7]
RP INTERACTION WITH ROCK1.
RX PubMed=11956230; DOI=10.1083/jcb.200111026;
RA Ward Y., Yap S.-F., Ravichandran V., Matsumura F., Ito M., Spinelli B.,
RA Kelly K.;
RT "The GTP binding proteins Gem and Rad are negative regulators of the Rho-
RT Rho kinase pathway.";
RL J. Cell Biol. 157:291-302(2002).
CC -!- FUNCTION: Could be a regulatory protein, possibly participating in
CC receptor-mediated signal transduction at the plasma membrane. Has
CC guanine nucleotide-binding activity but undetectable intrinsic GTPase
CC activity.
CC -!- SUBUNIT: Interacts with calmodulin in a Ca(2+)-dependent manner. Binds
CC ROCK1. {ECO:0000269|PubMed:11956230, ECO:0000269|PubMed:9115241}.
CC -!- INTERACTION:
CC P55040; O43865: AHCYL1; NbExp=8; IntAct=EBI-744104, EBI-2371423;
CC P55040; Q8N2N9-4: ANKRD36B; NbExp=3; IntAct=EBI-744104, EBI-12170453;
CC P55040; Q8WXK1: ASB15; NbExp=3; IntAct=EBI-744104, EBI-12809012;
CC P55040; Q9BUH8: BEGAIN; NbExp=5; IntAct=EBI-744104, EBI-742722;
CC P55040; Q6PI77: BHLHB9; NbExp=3; IntAct=EBI-744104, EBI-11519926;
CC P55040; Q6QNY1: BLOC1S2; NbExp=3; IntAct=EBI-744104, EBI-465872;
CC P55040; Q9H2G9: BLZF1; NbExp=6; IntAct=EBI-744104, EBI-2548012;
CC P55040; Q8NDD1-6: C1orf131; NbExp=3; IntAct=EBI-744104, EBI-17761821;
CC P55040; P54284: CACNB3; NbExp=8; IntAct=EBI-744104, EBI-1184651;
CC P55040; Q9H257-2: CARD9; NbExp=3; IntAct=EBI-744104, EBI-11530605;
CC P55040; Q68D86: CCDC102B; NbExp=3; IntAct=EBI-744104, EBI-10171570;
CC P55040; Q86Z20: CCDC125; NbExp=3; IntAct=EBI-744104, EBI-11977221;
CC P55040; A6NC98: CCDC88B; NbExp=3; IntAct=EBI-744104, EBI-347573;
CC P55040; Q01850: CDR2; NbExp=3; IntAct=EBI-744104, EBI-1181367;
CC P55040; Q8NHQ1: CEP70; NbExp=3; IntAct=EBI-744104, EBI-739624;
CC P55040; Q9Y2V7: COG6; NbExp=3; IntAct=EBI-744104, EBI-3866319;
CC P55040; Q96MW5: COG8; NbExp=3; IntAct=EBI-744104, EBI-720875;
CC P55040; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-744104, EBI-3867333;
CC P55040; Q8NFT6-2: DBF4B; NbExp=3; IntAct=EBI-744104, EBI-12205861;
CC P55040; Q05D60: DEUP1; NbExp=6; IntAct=EBI-744104, EBI-748597;
CC P55040; Q9NRI5-2: DISC1; NbExp=3; IntAct=EBI-744104, EBI-11988027;
CC P55040; Q9BQ95: ECSIT; NbExp=3; IntAct=EBI-744104, EBI-712452;
CC P55040; Q5JST6: EFHC2; NbExp=3; IntAct=EBI-744104, EBI-2349927;
CC P55040; Q96B26: EXOSC8; NbExp=3; IntAct=EBI-744104, EBI-371922;
CC P55040; Q8TAE8: GADD45GIP1; NbExp=3; IntAct=EBI-744104, EBI-372506;
CC P55040; O95995: GAS8; NbExp=3; IntAct=EBI-744104, EBI-1052570;
CC P55040; Q08379: GOLGA2; NbExp=6; IntAct=EBI-744104, EBI-618309;
CC P55040; A6NEM1: GOLGA6L9; NbExp=3; IntAct=EBI-744104, EBI-5916454;
CC P55040; Q9HD26-2: GOPC; NbExp=3; IntAct=EBI-744104, EBI-11102276;
CC P55040; Q96ED9-2: HOOK2; NbExp=3; IntAct=EBI-744104, EBI-10961706;
CC P55040; O75031: HSF2BP; NbExp=3; IntAct=EBI-744104, EBI-7116203;
CC P55040; Q8IYA8: IHO1; NbExp=3; IntAct=EBI-744104, EBI-8638439;
CC P55040; Q9UKT9: IKZF3; NbExp=8; IntAct=EBI-744104, EBI-747204;
CC P55040; Q0VD86: INCA1; NbExp=3; IntAct=EBI-744104, EBI-6509505;
CC P55040; Q8NBZ0: INO80E; NbExp=6; IntAct=EBI-744104, EBI-769401;
CC P55040; Q1MX18: INSC; NbExp=3; IntAct=EBI-744104, EBI-12081118;
CC P55040; Q86U28: ISCA2; NbExp=3; IntAct=EBI-744104, EBI-10258659;
CC P55040; Q9H079: KATNBL1; NbExp=5; IntAct=EBI-744104, EBI-715394;
CC P55040; Q7L273: KCTD9; NbExp=6; IntAct=EBI-744104, EBI-4397613;
CC P55040; Q9BVG8-5: KIFC3; NbExp=3; IntAct=EBI-744104, EBI-14069005;
CC P55040; Q15323: KRT31; NbExp=6; IntAct=EBI-744104, EBI-948001;
CC P55040; O76011: KRT34; NbExp=3; IntAct=EBI-744104, EBI-1047093;
CC P55040; O76013-2: KRT36; NbExp=3; IntAct=EBI-744104, EBI-11958506;
CC P55040; Q6A162: KRT40; NbExp=3; IntAct=EBI-744104, EBI-10171697;
CC P55040; P02538: KRT6A; NbExp=3; IntAct=EBI-744104, EBI-702198;
CC P55040; P78385: KRT83; NbExp=3; IntAct=EBI-744104, EBI-10221390;
CC P55040; P60409: KRTAP10-7; NbExp=6; IntAct=EBI-744104, EBI-10172290;
CC P55040; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-744104, EBI-10171774;
CC P55040; P48059-3: LIMS1; NbExp=3; IntAct=EBI-744104, EBI-12864460;
CC P55040; Q9P2M1: LRP2BP; NbExp=3; IntAct=EBI-744104, EBI-18273118;
CC P55040; Q9BRK4: LZTS2; NbExp=6; IntAct=EBI-744104, EBI-741037;
CC P55040; Q96EZ8: MCRS1; NbExp=3; IntAct=EBI-744104, EBI-348259;
CC P55040; Q14696: MESD; NbExp=3; IntAct=EBI-744104, EBI-6165891;
CC P55040; A0A0C4DFN3: MGLL; NbExp=3; IntAct=EBI-744104, EBI-12866138;
CC P55040; Q9UJV3-2: MID2; NbExp=4; IntAct=EBI-744104, EBI-10172526;
CC P55040; Q8TD10: MIPOL1; NbExp=3; IntAct=EBI-744104, EBI-2548751;
CC P55040; Q13064: MKRN3; NbExp=3; IntAct=EBI-744104, EBI-2340269;
CC P55040; Q6PF18: MORN3; NbExp=3; IntAct=EBI-744104, EBI-9675802;
CC P55040; Q4VC12: MSS51; NbExp=3; IntAct=EBI-744104, EBI-11599933;
CC P55040; Q5JR59-3: MTUS2; NbExp=6; IntAct=EBI-744104, EBI-11522433;
CC P55040; P15173: MYOG; NbExp=3; IntAct=EBI-744104, EBI-3906629;
CC P55040; P17568: NDUFB7; NbExp=3; IntAct=EBI-744104, EBI-1246238;
CC P55040; Q7Z3S9: NOTCH2NLA; NbExp=3; IntAct=EBI-744104, EBI-945833;
CC P55040; O43482: OIP5; NbExp=3; IntAct=EBI-744104, EBI-536879;
CC P55040; Q9BYU1: PBX4; NbExp=3; IntAct=EBI-744104, EBI-10302990;
CC P55040; Q9NR12: PDLIM7; NbExp=5; IntAct=EBI-744104, EBI-350517;
CC P55040; Q9NWS0: PIH1D1; NbExp=3; IntAct=EBI-744104, EBI-357318;
CC P55040; Q9UPG8: PLAGL2; NbExp=3; IntAct=EBI-744104, EBI-2876622;
CC P55040; Q9H8W4: PLEKHF2; NbExp=3; IntAct=EBI-744104, EBI-742388;
CC P55040; Q8ND90: PNMA1; NbExp=3; IntAct=EBI-744104, EBI-302345;
CC P55040; Q8WVV4-1: POF1B; NbExp=3; IntAct=EBI-744104, EBI-11986735;
CC P55040; Q9GZV8: PRDM14; NbExp=3; IntAct=EBI-744104, EBI-3957793;
CC P55040; Q9NQX0: PRDM6; NbExp=3; IntAct=EBI-744104, EBI-11320284;
CC P55040; Q59EK9: RUNDC3A; NbExp=3; IntAct=EBI-744104, EBI-747225;
CC P55040; O60641-3: SNAP91; NbExp=3; IntAct=EBI-744104, EBI-12854506;
CC P55040; O60504: SORBS3; NbExp=3; IntAct=EBI-744104, EBI-741237;
CC P55040; Q96R06: SPAG5; NbExp=3; IntAct=EBI-744104, EBI-413317;
CC P55040; Q9NWH7-2: SPATA6; NbExp=3; IntAct=EBI-744104, EBI-17860101;
CC P55040; Q9Y2D8: SSX2IP; NbExp=3; IntAct=EBI-744104, EBI-2212028;
CC P55040; Q6DHY5: TBC1D3G; NbExp=3; IntAct=EBI-744104, EBI-13092532;
CC P55040; P15884: TCF4; NbExp=3; IntAct=EBI-744104, EBI-533224;
CC P55040; Q01664: TFAP4; NbExp=2; IntAct=EBI-744104, EBI-2514218;
CC P55040; Q9UBB9: TFIP11; NbExp=7; IntAct=EBI-744104, EBI-1105213;
CC P55040; Q96PF1: TGM7; NbExp=3; IntAct=EBI-744104, EBI-12029034;
CC P55040; Q08117-2: TLE5; NbExp=3; IntAct=EBI-744104, EBI-11741437;
CC P55040; Q05BL1: TP53BP2; NbExp=3; IntAct=EBI-744104, EBI-11952721;
CC P55040; Q13077: TRAF1; NbExp=6; IntAct=EBI-744104, EBI-359224;
CC P55040; Q12933: TRAF2; NbExp=3; IntAct=EBI-744104, EBI-355744;
CC P55040; Q9UDY6-2: TRIM10; NbExp=3; IntAct=EBI-744104, EBI-11981577;
CC P55040; P36406: TRIM23; NbExp=7; IntAct=EBI-744104, EBI-740098;
CC P55040; P14373: TRIM27; NbExp=6; IntAct=EBI-744104, EBI-719493;
CC P55040; Q13049: TRIM32; NbExp=3; IntAct=EBI-744104, EBI-742790;
CC P55040; Q9BYV2: TRIM54; NbExp=3; IntAct=EBI-744104, EBI-2130429;
CC P55040; Q86WT6-2: TRIM69; NbExp=3; IntAct=EBI-744104, EBI-11525489;
CC P55040; Q495M9: USH1G; NbExp=3; IntAct=EBI-744104, EBI-8601749;
CC P55040; Q8N6Y0: USHBP1; NbExp=3; IntAct=EBI-744104, EBI-739895;
CC P55040; P08670: VIM; NbExp=3; IntAct=EBI-744104, EBI-353844;
CC P55040; Q9UK41-2: VPS28; NbExp=3; IntAct=EBI-744104, EBI-12146727;
CC P55040; Q8N1B4: VPS52; NbExp=3; IntAct=EBI-744104, EBI-2799833;
CC P55040; Q9NZC7-5: WWOX; NbExp=3; IntAct=EBI-744104, EBI-12040603;
CC P55040; P31946: YWHAB; NbExp=4; IntAct=EBI-744104, EBI-359815;
CC P55040; B2RXF5: ZBTB42; NbExp=3; IntAct=EBI-744104, EBI-12287587;
CC P55040; Q9NQZ6: ZC4H2; NbExp=3; IntAct=EBI-744104, EBI-747993;
CC P55040; Q9UDW3: ZMAT5; NbExp=3; IntAct=EBI-744104, EBI-7850213;
CC P55040; P17024: ZNF20; NbExp=3; IntAct=EBI-744104, EBI-717634;
CC P55040; Q9H707: ZNF552; NbExp=3; IntAct=EBI-744104, EBI-2555731;
CC P55040; Q8N720: ZNF655; NbExp=3; IntAct=EBI-744104, EBI-625509;
CC P55040; P0C7X2: ZNF688; NbExp=3; IntAct=EBI-744104, EBI-4395732;
CC P55040; Q6NX45: ZNF774; NbExp=3; IntAct=EBI-744104, EBI-10251462;
CC P55040; Q9UGI0: ZRANB1; NbExp=3; IntAct=EBI-744104, EBI-527853;
CC P55040; O15535: ZSCAN9; NbExp=3; IntAct=EBI-744104, EBI-751531;
CC P55040; P27884: CACNA1A; Xeno; NbExp=2; IntAct=EBI-744104, EBI-15685548;
CC P55040; P54287: Cacnb3; Xeno; NbExp=2; IntAct=EBI-744104, EBI-8028596;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein;
CC Cytoplasmic side.
CC -!- TISSUE SPECIFICITY: Most abundant in thymus, spleen, kidney, lung, and
CC testis. Less abundant in heart, brain, liver and skeletal muscle.
CC -!- INDUCTION: By mitogens.
CC -!- PTM: Phosphorylated on tyrosine residues.
CC -!- SIMILARITY: Belongs to the small GTPase superfamily. RGK family.
CC {ECO:0000305}.
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DR EMBL; U10550; AAA64911.1; -; mRNA.
DR EMBL; U13052; AAC50067.1; -; mRNA.
DR EMBL; AK314017; BAG36728.1; -; mRNA.
DR EMBL; CH471060; EAW91709.1; -; Genomic_DNA.
DR EMBL; BC022010; AAH22010.1; -; mRNA.
DR CCDS; CCDS6261.1; -.
DR PIR; A54575; A54575.
DR PIR; I38745; I38745.
DR RefSeq; NP_005252.1; NM_005261.3.
DR RefSeq; NP_859053.1; NM_181702.2.
DR PDB; 2CJW; X-ray; 2.10 A; A/B=74-261.
DR PDB; 2G3Y; X-ray; 2.40 A; A=62-249.
DR PDB; 2HT6; X-ray; 2.40 A; A/B=71-243.
DR PDBsum; 2CJW; -.
DR PDBsum; 2G3Y; -.
DR PDBsum; 2HT6; -.
DR AlphaFoldDB; P55040; -.
DR SMR; P55040; -.
DR BioGRID; 108937; 148.
DR DIP; DIP-41587N; -.
DR IntAct; P55040; 148.
DR MINT; P55040; -.
DR STRING; 9606.ENSP00000297596; -.
DR iPTMnet; P55040; -.
DR PhosphoSitePlus; P55040; -.
DR BioMuta; GEM; -.
DR DMDM; 1707896; -.
DR EPD; P55040; -.
DR jPOST; P55040; -.
DR MassIVE; P55040; -.
DR MaxQB; P55040; -.
DR PaxDb; P55040; -.
DR PeptideAtlas; P55040; -.
DR PRIDE; P55040; -.
DR ProteomicsDB; 56765; -.
DR Antibodypedia; 12852; 178 antibodies from 34 providers.
DR DNASU; 2669; -.
DR Ensembl; ENST00000297596.3; ENSP00000297596.2; ENSG00000164949.8.
DR Ensembl; ENST00000396194.6; ENSP00000379497.2; ENSG00000164949.8.
DR GeneID; 2669; -.
DR KEGG; hsa:2669; -.
DR MANE-Select; ENST00000297596.3; ENSP00000297596.2; NM_005261.4; NP_005252.1.
DR UCSC; uc003ygi.4; human.
DR CTD; 2669; -.
DR DisGeNET; 2669; -.
DR GeneCards; GEM; -.
DR HGNC; HGNC:4234; GEM.
DR HPA; ENSG00000164949; Tissue enhanced (gallbladder).
DR MIM; 600164; gene.
DR neXtProt; NX_P55040; -.
DR OpenTargets; ENSG00000164949; -.
DR PharmGKB; PA28645; -.
DR VEuPathDB; HostDB:ENSG00000164949; -.
DR eggNOG; KOG0395; Eukaryota.
DR GeneTree; ENSGT00940000157830; -.
DR HOGENOM; CLU_041217_3_2_1; -.
DR InParanoid; P55040; -.
DR OMA; SNEYNPQ; -.
DR OrthoDB; 679855at2759; -.
DR PhylomeDB; P55040; -.
DR TreeFam; TF314379; -.
DR PathwayCommons; P55040; -.
DR SignaLink; P55040; -.
DR SIGNOR; P55040; -.
DR BioGRID-ORCS; 2669; 12 hits in 1080 CRISPR screens.
DR ChiTaRS; GEM; human.
DR EvolutionaryTrace; P55040; -.
DR GeneWiki; GEM_(gene); -.
DR GenomeRNAi; 2669; -.
DR Pharos; P55040; Tbio.
DR PRO; PR:P55040; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; P55040; protein.
DR Bgee; ENSG00000164949; Expressed in pigmented layer of retina and 187 other tissues.
DR ExpressionAtlas; P55040; baseline and differential.
DR Genevisible; P55040; HS.
DR GO; GO:0009898; C:cytoplasmic side of plasma membrane; IDA:BHF-UCL.
DR GO; GO:0030496; C:midbody; IDA:UniProtKB.
DR GO; GO:0072686; C:mitotic spindle; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0051233; C:spindle midzone; IDA:UniProtKB.
DR GO; GO:0005246; F:calcium channel regulator activity; IBA:GO_Central.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0019003; F:GDP binding; IDA:BHF-UCL.
DR GO; GO:0005525; F:GTP binding; IDA:BHF-UCL.
DR GO; GO:0003924; F:GTPase activity; IDA:BHF-UCL.
DR GO; GO:0000287; F:magnesium ion binding; IDA:BHF-UCL.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0051276; P:chromosome organization; IMP:UniProtKB.
DR GO; GO:0006955; P:immune response; TAS:ProtInc.
DR GO; GO:0051310; P:metaphase plate congression; IMP:UniProtKB.
DR GO; GO:0000278; P:mitotic cell cycle; IMP:UniProtKB.
DR GO; GO:1901842; P:negative regulation of high voltage-gated calcium channel activity; IEA:InterPro.
DR GO; GO:0007165; P:signal transduction; TAS:ProtInc.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR017358; RGK.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR001806; Small_GTPase.
DR Pfam; PF00071; Ras; 1.
DR PIRSF; PIRSF038017; GTP-binding_GEM; 1.
DR SMART; SM00174; RHO; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00231; small_GTP; 1.
DR PROSITE; PS51421; RAS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Calmodulin-binding; Cell membrane; GTP-binding; Membrane;
KW Nucleotide-binding; Phosphoprotein; Reference proteome.
FT CHAIN 1..296
FT /note="GTP-binding protein GEM"
FT /id="PRO_0000122475"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 37..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 266..285
FT /note="Calmodulin-binding"
FT /evidence="ECO:0000250"
FT COMPBIAS 37..59
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 82..89
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 191..194
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT VARIANT 43
FT /note="R -> G (in dbSNP:rs2170363)"
FT /id="VAR_020097"
FT CONFLICT 159
FT /note="I -> V (in Ref. 2; AAC50067)"
FT /evidence="ECO:0000305"
FT CONFLICT 174..175
FT /note="QL -> HV (in Ref. 2; AAC50067)"
FT /evidence="ECO:0000305"
FT STRAND 75..81
FT /evidence="ECO:0007829|PDB:2CJW"
FT HELIX 88..98
FT /evidence="ECO:0007829|PDB:2CJW"
FT HELIX 107..109
FT /evidence="ECO:0007829|PDB:2CJW"
FT STRAND 113..120
FT /evidence="ECO:0007829|PDB:2CJW"
FT STRAND 123..130
FT /evidence="ECO:0007829|PDB:2CJW"
FT TURN 133..136
FT /evidence="ECO:0007829|PDB:2G3Y"
FT HELIX 143..145
FT /evidence="ECO:0007829|PDB:2CJW"
FT HELIX 146..149
FT /evidence="ECO:0007829|PDB:2CJW"
FT STRAND 151..158
FT /evidence="ECO:0007829|PDB:2CJW"
FT HELIX 162..178
FT /evidence="ECO:0007829|PDB:2CJW"
FT TURN 179..181
FT /evidence="ECO:0007829|PDB:2CJW"
FT STRAND 186..191
FT /evidence="ECO:0007829|PDB:2CJW"
FT HELIX 196..198
FT /evidence="ECO:0007829|PDB:2CJW"
FT HELIX 203..212
FT /evidence="ECO:0007829|PDB:2CJW"
FT STRAND 216..219
FT /evidence="ECO:0007829|PDB:2CJW"
FT TURN 222..225
FT /evidence="ECO:0007829|PDB:2CJW"
FT HELIX 228..243
FT /evidence="ECO:0007829|PDB:2CJW"
FT HELIX 246..257
FT /evidence="ECO:0007829|PDB:2CJW"
SQ SEQUENCE 296 AA; 33949 MW; B7BB3F2CA96D338F CRC64;
MTLNNVTMRQ GTVGMQPQQQ RWSIPADGRH LMVQKEPHQY SHRNRHSATP EDHCRRSWSS
DSTDSVISSE SGNTYYRVVL IGEQGVGKST LANIFAGVHD SMDSDCEVLG EDTYERTLMV
DGESATIILL DMWENKGENE WLHDHCMQVG DAYLIVYSIT DRASFEKASE LRIQLRRARQ
TEDIPIILVG NKSDLVRCRE VSVSEGRACA VVFDCKFIET SAAVQHNVKE LFEGIVRQVR
LRRDSKEKNE RRLAYQKRKE SMPRKARRFW GKIVAKNNKN MAFKLKSKSC HDLSVL
