GENK_CORGL
ID GENK_CORGL Reviewed; 444 AA.
AC Q8NLB7; Q6M1I5;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 2.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Gentisate transporter;
GN Name=genK; OrderedLocusNames=Cgl3025, cg3353;
OS Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 /
OS JCM 1318 / LMG 3730 / NCIMB 10025).
OC Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC Corynebacterium.
OX NCBI_TaxID=196627;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=12743753; DOI=10.1007/s00253-003-1328-1;
RA Ikeda M., Nakagawa S.;
RT "The Corynebacterium glutamicum genome: features and impacts on
RT biotechnological processes.";
RL Appl. Microbiol. Biotechnol. 62:99-109(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=12948626; DOI=10.1016/s0168-1656(03)00154-8;
RA Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A.,
RA Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A.,
RA Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F.,
RA Moeckel B., Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O.,
RA Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.;
RT "The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its
RT impact on the production of L-aspartate-derived amino acids and vitamins.";
RL J. Biotechnol. 104:5-25(2003).
RN [3]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, DISRUPTION PHENOTYPE, AND
RP MUTAGENESIS OF ASP-54; ASP-57; ARG-103; TRP-309; ASP-312; ARG-313; ILE-317
RP AND ARG-386.
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=22808015; DOI=10.1371/journal.pone.0038701;
RA Xu Y., Wang S.H., Chao H.J., Liu S.J., Zhou N.Y.;
RT "Biochemical and molecular characterization of the gentisate transporter
RT GenK in Corynebacterium glutamicum.";
RL PLoS ONE 7:E38701-E38701(2012).
CC -!- FUNCTION: Transport of gentisate (2,5-dihydroxybenzoate) into the cell.
CC Does not transport 3-hydroxybenzoate or benzoate.
CC {ECO:0000269|PubMed:22808015}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=10.71 uM for gentisate {ECO:0000269|PubMed:22808015};
CC Vmax=3.06 nmol/min/mg enzyme {ECO:0000269|PubMed:22808015};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutant retains 85% of its transport
CC activity at pH 6.5, but it loses 79% and 88% activity at pH 7.5 and
CC 8.0, respectively. {ECO:0000269|PubMed:22808015}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Aromatic
CC acid:H(+) symporter (AAHS) (TC 2.A.1.15) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC00419.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAF18965.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; BA000036; BAC00419.1; ALT_INIT; Genomic_DNA.
DR EMBL; BX927157; CAF18965.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_602219.1; NC_003450.3.
DR RefSeq; WP_011015577.1; NC_003450.3.
DR AlphaFoldDB; Q8NLB7; -.
DR SMR; Q8NLB7; -.
DR STRING; 196627.cg3353; -.
DR TCDB; 2.A.1.15.10; the major facilitator superfamily (mfs).
DR KEGG; cgb:cg3353; -.
DR KEGG; cgl:Cgl3025; -.
DR PATRIC; fig|196627.13.peg.2960; -.
DR eggNOG; COG2814; Bacteria.
DR HOGENOM; CLU_001265_46_4_11; -.
DR BioCyc; MetaCyc:G18NG-12646-MON; -.
DR Proteomes; UP000000582; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..444
FT /note="Gentisate transporter"
FT /id="PRO_0000428627"
FT TRANSMEM 42..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 318..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..366
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 410..428
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 54
FT /note="D->A: Loss of transport activity."
FT /evidence="ECO:0000269|PubMed:22808015"
FT MUTAGEN 54
FT /note="D->E: Retains 50% of its transport activity."
FT /evidence="ECO:0000269|PubMed:22808015"
FT MUTAGEN 57
FT /note="D->A: Loss of transport activity."
FT /evidence="ECO:0000269|PubMed:22808015"
FT MUTAGEN 57
FT /note="D->E: Retains 50% of its transport activity."
FT /evidence="ECO:0000269|PubMed:22808015"
FT MUTAGEN 103
FT /note="R->A: Loss of transport activity."
FT /evidence="ECO:0000269|PubMed:22808015"
FT MUTAGEN 309
FT /note="W->V: Loss of transport activity."
FT /evidence="ECO:0000269|PubMed:22808015"
FT MUTAGEN 312
FT /note="D->A: Loss of transport activity."
FT /evidence="ECO:0000269|PubMed:22808015"
FT MUTAGEN 313
FT /note="R->A: Loss of transport activity."
FT /evidence="ECO:0000269|PubMed:22808015"
FT MUTAGEN 317
FT /note="I->H,Y: Loss of transport activity."
FT /evidence="ECO:0000269|PubMed:22808015"
FT MUTAGEN 386
FT /note="R->A: Loss of transport activity."
FT /evidence="ECO:0000269|PubMed:22808015"
SQ SEQUENCE 444 AA; 46806 MW; ABFD4C5D5F73608E CRC64;
MTSHAPESGG LVTESTLGAS NSSQTIENKG LTILGISGRR LAAVLIGWFF VIFDGYDLIV
YGTVQSALAK EWNLSSATLG TIGSTAFFGM AIGAVFIGRL SDRVGRKAAV IGSVLILSVF
TMLCAFAPNP WVFGAFRFIA GLGLGGLVPS VNAMTSDLVP RKTMSAWATV MMSGVPIGGS
IAAVLALVVV PSSEEWGWRF MFLIALIPLV VGLPIAMKVI PSDKAIKADH DIREGHDEPA
GFKDLLVDRY RWISIWFALA TFVTLLAWYG LGTWLPRLME TAGYEFGHAL MFTLALNLGA
VIGSVVTAWA GDRFGPIRSG VIAAGIAGIA LLLLLTYPPV TAVYVILILA GVGTHGTQIL
IIAAVANFYP SNLRGTALGW ALGVGRIGAV VAPQLAGLLL AWNLGVNSNF IMFGTAALLS
ALALSVLLRL QKTYSVTHKV EIQG
