ID   ALPS_LIMPO              Reviewed;         101 AA.
AC   P07086;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   25-MAY-2022, entry version 46.
DE   RecName: Full=Anti-lipopolysaccharide factor;
DE            Short=anti-LPS;
DE   AltName: Full=LALF;
OS   Limulus polyphemus (Atlantic horseshoe crab).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Merostomata;
OC   Xiphosura; Limulidae; Limulus.
OX   NCBI_TaxID=6850;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=3667549; DOI=10.1093/oxfordjournals.jbchem.a121999;
RA   Muta T., Miyata T., Tokunaga F., Nakamura T., Iwanaga S.;
RT   "Primary structure of anti-lipopolysaccharide factor from American
RT   horseshoe crab, Limulus polyphemus.";
RL   J. Biochem. 101:1321-1330(1987).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).
RX   PubMed=8253062; DOI=10.1002/j.1460-2075.1993.tb06008.x;
RA   Hoess A., Watson S., Siber G.R., Liddington R.;
RT   "Crystal structure of an endotoxin-neutralizing protein from the horseshoe
RT   crab, Limulus anti-LPS factor, at 1.5-A resolution.";
RL   EMBO J. 12:3351-3356(1993).
CC   -!- FUNCTION: Binds tightly to LPS and thus specifically inhibits the LPS-
CC       mediated activation of the hemolymph coagulation. It has a strong
CC       antibacterial effect especially on the growth of Gram-negative
CC       bacteria.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PIR; A27819; A27819.
DR   AlphaFoldDB; P07086; -.
DR   SMR; P07086; -.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.160.320; -; 1.
DR   InterPro; IPR024509; Anti-LPS_factor/Scygonadin.
DR   InterPro; IPR038539; Anti-LPS_factor/Scygonadin_sf.
DR   Pfam; PF11630; DUF3254; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond.
FT   CHAIN           1..101
FT                   /note="Anti-lipopolysaccharide factor"
FT                   /id="PRO_0000064572"
FT   DISULFID        31..52
FT   VARIANT         13
FT                   /note="K -> N"
SQ   SEQUENCE   101 AA;  11801 MW;  76D6449AAE18B769 CRC64;
     DGIWTQLIFT LVKNLATLWQ SGDFQFLDHE CHYRIKPTFR RLKWKYKGKF WCPSWTSITG
     RATKSSRSGA VEHSVRNFVG QAKSSGLITQ RQAEQFISQY N